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OPENSEQ.org
MDH - Malate dehydrogenase
UniProt: P61889 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10576
Length: 312 (301)
Sequences: 2652
Seq/Len: 8.81
MDH
Paralog alert: 0.37 [within 20: 0.03] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
4_A 70_A 4.022 1.00
72_V 235_G 2.968 1.00
289_Q 293_E 2.949 1.00
74_L 235_G 2.707 1.00
204_K 208_N 2.594 1.00
117_I 231_A 2.483 1.00
67_L 109_C 2.459 1.00
297_D 301_K 2.457 1.00
95_A 127_I 2.443 1.00
269_L 277_E 2.399 1.00
205_R 212_E 2.327 1.00
79_V 93_V 2.296 1.00
4_A 67_L 2.211 1.00
104_Q 107_K 2.09 1.00
246_Q 271_G 2.069 1.00
304_A 308_E 2.017 1.00
99_K 103_Q 2.007 1.00
286_A 289_Q 1.997 1.00
264_F 292_L 1.988 1.00
36_A 39_T 1.947 1.00
290_N 294_G 1.935 1.00
61_E 65_P 1.932 1.00
246_Q 276_E 1.911 1.00
293_E 297_D 1.887 1.00
23_Q 236_L 1.854 1.00
29_E 54_K 1.824 1.00
264_F 296_L 1.816 1.00
271_G 276_E 1.804 1.00
140_K 255_E 1.794 1.00
106_A 136_G 1.79 1.00
106_A 137_V 1.787 1.00
286_A 290_N 1.745 1.00
159_A 169_V 1.729 1.00
64_T 107_K 1.721 1.00
174_I 185_L 1.689 1.00
201_D 205_R 1.651 1.00
47_S 160_E 1.65 1.00
64_T 108_T 1.648 1.00
65_P 68_E 1.646 1.00
254_V 266_Q 1.627 1.00
81_R 210_G 1.616 1.00
100_N 104_Q 1.614 1.00
259_Q 292_L 1.597 1.00
253_Y 263_F 1.586 1.00
102_V 135_A 1.585 1.00
103_Q 107_K 1.585 1.00
137_V 142_K 1.571 1.00
2_K 31_S 1.571 1.00
100_N 103_Q 1.56 1.00
236_L 243_Q 1.545 1.00
113_C 238_L 1.527 1.00
273_N 276_E 1.515 1.00
103_Q 134_K 1.509 1.00
3_V 20_L 1.503 1.00
291_A 294_G 1.496 1.00
85_M 93_V 1.493 1.00
252_A 268_L 1.486 1.00
91_F 302_D 1.485 1.00
167_G 245_E 1.482 1.00
161_L 198_E 1.482 1.00
73_V 105_V 1.481 1.00
183_L 288_E 1.474 1.00
5_V 17_A 1.472 1.00
259_Q 293_E 1.467 1.00
2_K 29_E 1.465 1.00
146_V 231_A 1.444 0.99
54_K 160_E 1.442 0.99
113_C 142_K 1.438 0.99
111_K 139_D 1.43 0.99
69_G 111_K 1.419 0.99
132_L 263_F 1.417 0.99
268_L 275_V 1.412 0.99
200_A 204_K 1.402 0.99
165_Q 168_E 1.388 0.99
147_T 265_S 1.372 0.99
254_V 257_D 1.371 0.99
47_S 156_T 1.369 0.99
67_L 70_A 1.354 0.99
141_N 273_N 1.354 0.99
181_T 298_T 1.353 0.99
196_E 200_A 1.331 0.99
49_I 229_Q 1.324 0.99
204_K 207_Q 1.299 0.99
8_A 32_L 1.294 0.99
164_K 169_V 1.284 0.98
16_L 231_A 1.283 0.98
184_P 203_T 1.28 0.98
126_A 303_I 1.274 0.98
125_V 263_F 1.274 0.98
292_L 296_L 1.271 0.98
93_V 96_G 1.268 0.98
98_V 131_V 1.261 0.98
300_K 304_A 1.26 0.98
173_V 184_P 1.247 0.98
248_V 269_L 1.236 0.98
90_L 94_N 1.232 0.98
187_S 288_E 1.231 0.98
20_L 30_L 1.228 0.98
208_N 211_T 1.225 0.98
102_V 128_A 1.222 0.98
86_D 89_D 1.216 0.98
294_G 297_D 1.215 0.98
18_L 228_G 1.214 0.98
109_C 112_A 1.201 0.97
121_V 145_G 1.194 0.97
148_T 230_A 1.192 0.97
252_A 275_V 1.188 0.97
119_N 177_H 1.185 0.97
244_G 272_K 1.171 0.97
105_V 137_V 1.166 0.97
48_H 209_A 1.158 0.96
208_N 215_E 1.158 0.96
102_V 114_I 1.157 0.96
105_V 114_I 1.156 0.96
81_R 85_M 1.155 0.96
11_G 224_T 1.154 0.96
255_E 260_Y 1.154 0.96
287_F 290_N 1.15 0.96
210_G 214_V 1.15 0.96
290_N 293_E 1.146 0.96
187_S 285_S 1.145 0.96
102_V 131_V 1.144 0.96
89_D 92_N 1.14 0.96
189_V 194_F 1.136 0.96
251_C 268_L 1.131 0.96
96_G 99_K 1.123 0.96
79_V 82_K 1.122 0.96
14_Q 42_V 1.122 0.96
301_K 304_A 1.12 0.96
72_V 238_L 1.118 0.95
301_K 305_L 1.113 0.95
115_G 235_G 1.106 0.95
148_T 249_V 1.1 0.95
18_L 22_T 1.097 0.95
186_L 206_I 1.087 0.94
212_E 216_A 1.086 0.94
266_Q 278_R 1.08 0.94
126_A 133_K 1.076 0.94
269_L 279_K 1.074 0.94
41_G 216_A 1.067 0.94
195_T 198_E 1.066 0.94
155_N 170_E 1.062 0.94
79_V 97_I 1.05 0.93
140_K 253_Y 1.046 0.93
212_E 215_E 1.043 0.93
5_V 20_L 1.042 0.93
70_A 73_V 1.041 0.93
68_E 108_T 1.039 0.93
81_R 214_V 1.037 0.92
74_L 115_G 1.034 0.92
125_V 145_G 1.032 0.92
143_L 263_F 1.03 0.92
81_R 211_T 1.027 0.92
168_E 190_P 1.027 0.92
87_R 119_N 1.026 0.92
26_S 243_Q 1.026 0.92
171_V 189_V 1.025 0.92
102_V 106_A 1.023 0.92
280_S 283_T 1.022 0.92
98_V 102_V 1.021 0.92
129_A 143_L 1.02 0.92
3_V 27_G 1.02 0.92
83_P 215_E 1.019 0.92
161_L 201_D 1.018 0.92
87_R 177_H 1.011 0.91
60_G 66_A 1.011 0.91
196_E 287_F 1.011 0.91
85_M 89_D 1.009 0.91
97_I 118_T 1.006 0.91
259_Q 296_L 1 0.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3hhpA411000.194Contact Map0.666
3nepX10.94871000.251Contact Map0.675
2d4aB40.93591000.258Contact Map0.773
1guzA40.94871000.26Contact Map0.833
4bgtA20.93911000.263Contact Map0.731
3vpgA40.92951000.265Contact Map0.751
7mdhA40.95511000.267Contact Map0.62
1t2dA10.94231000.269Contact Map0.7
1a5zA10.93911000.271Contact Map0.717
1mldA40.98721000.271Contact Map0.705
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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