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OPENSEQ.org

RL4 - 50S ribosomal protein L4
UniProt: P60723 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10867
Length: 201 (192)
Sequences: 1779
Seq/Len: 9.27

RL4
Paralog alert: 0.06 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
45_A 88_R 3.556 1.00
60_W 78_W 3.301 1.00
70_S 77_I 3.184 1.00
45_A 51_E 3.156 1.00
28_V 108_I 2.88 1.00
134_L 161_A 2.547 1.00
190_A 194_K 2.467 1.00
55_S 59_P 2.178 1.00
29_H 33_V 2.061 1.00
51_E 88_R 2.029 1.00
46_Q 86_A 1.956 1.00
31_V 96_V 1.923 1.00
60_W 65_T 1.88 1.00
26_A 30_Q 1.872 1.00
31_V 104_A 1.865 1.00
133_L 136_Q 1.832 1.00
34_A 94_Q 1.827 1.00
70_S 75_S 1.79 1.00
52_V 82_G 1.777 1.00
45_A 89_P 1.767 1.00
55_S 73_I 1.668 1.00
117_R 185_K 1.663 1.00
48_T 51_E 1.661 1.00
159_L 162_R 1.643 1.00
136_Q 140_D 1.643 1.00
30_Q 100_M 1.609 1.00
36_A 178_V 1.608 1.00
27_L 103_G 1.591 1.00
97_N 100_M 1.585 1.00
128_A 133_L 1.551 1.00
96_V 100_M 1.546 1.00
54_G 74_K 1.543 1.00
135_A 139_K 1.532 1.00
27_L 100_M 1.515 1.00
188_M 196_V 1.497 1.00
28_V 107_S 1.474 1.00
132_K 136_Q 1.472 1.00
60_W 67_R 1.47 1.00
34_A 96_V 1.465 1.00
32_V 181_I 1.458 1.00
129_P 156_N 1.453 1.00
35_Y 96_V 1.431 0.99
56_G 74_K 1.428 0.99
30_Q 94_Q 1.424 0.99
70_S 78_W 1.42 0.99
44_R 92_H 1.417 0.99
151_G 191_D 1.408 0.99
77_I 84_T 1.379 0.99
119_I 185_K 1.374 0.99
35_Y 39_A 1.374 0.99
24_N 27_L 1.367 0.99
35_Y 179_S 1.359 0.99
60_W 70_S 1.332 0.99
28_V 181_I 1.292 0.99
55_S 74_K 1.292 0.99
59_P 78_W 1.286 0.99
125_S 154_D 1.283 0.99
175_I 196_V 1.283 0.99
35_Y 176_D 1.269 0.98
134_L 148_I 1.246 0.98
59_P 70_S 1.243 0.98
15_S 197_E 1.228 0.98
122_E 190_A 1.226 0.98
134_L 137_K 1.217 0.98
126_V 137_K 1.198 0.98
129_P 160_A 1.183 0.97
136_Q 139_K 1.173 0.97
191_D 194_K 1.167 0.97
40_R 92_H 1.163 0.97
56_G 59_P 1.16 0.97
135_A 164_L 1.153 0.97
101_Y 177_P 1.152 0.97
75_S 78_W 1.148 0.97
16_E 20_G 1.133 0.96
113_V 118_L 1.132 0.96
194_K 197_E 1.131 0.96
33_V 36_A 1.129 0.96
173_T 195_Q 1.114 0.96
129_P 159_L 1.112 0.96
36_A 39_A 1.109 0.96
188_M 193_V 1.108 0.96
172_A 195_Q 1.09 0.95
60_W 77_I 1.086 0.95
194_K 198_E 1.078 0.95
156_N 159_L 1.071 0.94
37_A 93_S 1.07 0.94
110_S 114_R 1.068 0.94
121_V 124_F 1.068 0.94
21_R 103_G 1.052 0.94
55_S 72_S 1.051 0.94
161_A 169_V 1.047 0.94
38_G 93_S 1.045 0.93
168_D 183_F 1.04 0.93
59_P 72_S 1.035 0.93
55_S 78_W 1.023 0.92
54_G 73_I 1.007 0.92
44_R 86_A 1.006 0.92
56_G 72_S 1.005 0.92
35_Y 177_P 1.004 0.92
57_K 73_I 1.002 0.91
34_A 38_G 1.001 0.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4kixE10.9951000.05Contact Map0.598
3j3wE111000.059Contact Map0.448
1dmgA111000.069Contact Map0.737
3bboG10.991000.095Contact Map0.364
3v2dF10.96521000.104Contact Map0.629
2zjrC10.96021000.11Contact Map0.435
2ftcD10.84581000.154Contact Map0.014
3j39C10.991000.18Contact Map0.436
4bynC10.98511000.189Contact Map0.429
4a17C10.98011000.203Contact Map0.429

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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