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OPENSEQ.org
CSIE - Stationary phase-inducible protein CsiE
UniProt: P54901 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13418
Length: 426 (413)
Sequences: 1117
Seq/Len: 2.70
CSIE
Paralog alert: 0.61 [within 20: 0.04] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
16_R 50_D 3.759 1.00
42_V 47_A 3.647 1.00
289_A 316_D 3.647 1.00
64_L 78_G 3.499 1.00
285_P 320_G 3.222 1.00
256_E 332_Q 3.078 1.00
80_V 164_H 2.965 1.00
23_T 39_L 2.682 1.00
42_V 50_D 2.651 1.00
299_T 323_A 2.547 1.00
55_G 68_T 2.514 1.00
289_A 296_V 2.462 1.00
253_F 326_F 2.441 1.00
246_R 252_R 2.427 1.00
42_V 46_I 2.414 1.00
84_R 207_E 2.398 1.00
345_T 348_D 2.348 1.00
256_E 329_W 2.231 1.00
356_E 360_R 2.13 1.00
340_Q 370_K 2.078 1.00
62_H 82_N 2.072 1.00
64_L 76_V 2.065 1.00
157_Q 214_L 2.048 1.00
67_T 77_E 2.032 1.00
139_R 190_E 1.956 0.99
80_V 84_R 1.941 0.99
44_D 73_S 1.865 0.99
17_R 54_T 1.861 0.99
245_F 326_F 1.826 0.99
32_T 73_S 1.806 0.99
300_R 316_D 1.78 0.99
303_L 322_V 1.778 0.99
259_L 263_L 1.765 0.98
348_D 351_R 1.731 0.98
353_A 356_E 1.705 0.98
57_E 86_C 1.692 0.98
405_I 415_Q 1.636 0.97
245_F 299_T 1.628 0.97
355_I 392_P 1.623 0.97
153_C 157_Q 1.607 0.97
259_L 329_W 1.593 0.97
267_L 270_A 1.582 0.96
32_T 48_S 1.573 0.96
282_N 320_G 1.559 0.96
273_R 318_E 1.559 0.96
345_T 350_E 1.556 0.96
283_T 317_E 1.555 0.96
343_L 359_L 1.548 0.96
345_T 352_E 1.543 0.96
295_L 299_T 1.542 0.96
408_D 415_Q 1.533 0.95
396_P 406_Y 1.53 0.95
390_I 405_I 1.527 0.95
180_E 205_P 1.518 0.95
390_I 407_T 1.503 0.95
239_K 260_C 1.494 0.95
249_G 298_T 1.488 0.94
79_T 82_N 1.47 0.94
240_R 309_E 1.465 0.94
88_F 157_Q 1.449 0.93
78_G 83_Q 1.444 0.93
266_H 325_I 1.436 0.93
196_Q 202_P 1.414 0.92
419_I 423_L 1.412 0.92
263_L 322_V 1.412 0.92
270_A 273_R 1.405 0.92
256_E 331_M 1.403 0.92
241_L 305_G 1.393 0.91
148_D 151_F 1.386 0.91
33_M 44_D 1.385 0.91
61_Y 89_H 1.385 0.91
245_F 298_T 1.381 0.91
204_P 207_E 1.38 0.91
21_L 54_T 1.343 0.89
69_G 72_G 1.342 0.89
241_L 299_T 1.336 0.89
329_W 332_Q 1.331 0.89
242_V 299_T 1.327 0.89
391_A 406_Y 1.327 0.89
347_N 351_R 1.318 0.88
54_T 66_L 1.312 0.88
415_Q 418_Q 1.305 0.87
282_N 317_E 1.304 0.87
259_L 332_Q 1.29 0.87
325_I 332_Q 1.277 0.86
358_Q 410_T 1.275 0.86
188_A 215_F 1.272 0.86
348_D 354_Q 1.27 0.85
296_V 300_R 1.27 0.85
345_T 351_R 1.269 0.85
137_S 144_F 1.268 0.85
269_Q 273_R 1.265 0.85
253_F 329_W 1.264 0.85
37_S 47_A 1.263 0.85
32_T 44_D 1.261 0.85
391_A 397_L 1.257 0.85
246_R 256_E 1.256 0.85
294_R 299_T 1.246 0.84
30_T 75_R 1.239 0.84
345_T 349_S 1.239 0.84
287_E 291_L 1.233 0.83
339_K 423_L 1.228 0.83
248_L 301_A 1.225 0.83
411_L 419_I 1.221 0.82
158_Y 211_M 1.22 0.82
341_I 390_I 1.213 0.82
282_N 321_L 1.209 0.81
270_A 274_S 1.204 0.81
238_I 267_L 1.203 0.81
307_E 313_H 1.202 0.81
325_I 328_A 1.2 0.81
241_L 326_F 1.2 0.81
19_Q 40_N 1.195 0.80
84_R 164_H 1.194 0.80
341_I 388_L 1.194 0.80
44_D 48_S 1.192 0.80
64_L 83_Q 1.191 0.80
164_H 168_T 1.186 0.80
273_R 280_I 1.182 0.79
274_S 312_V 1.179 0.79
328_A 332_Q 1.177 0.79
355_I 407_T 1.176 0.79
244_H 305_G 1.173 0.79
295_L 327_G 1.168 0.78
16_R 42_V 1.157 0.77
129_L 159_C 1.15 0.77
153_C 214_L 1.14 0.76
299_T 302_A 1.138 0.76
299_T 303_L 1.135 0.75
161_L 204_P 1.133 0.75
391_A 404_L 1.131 0.75
249_G 253_F 1.13 0.75
196_Q 199_A 1.126 0.75
185_F 208_P 1.119 0.74
242_V 259_L 1.118 0.74
185_F 212_A 1.102 0.72
44_D 350_E 1.102 0.72
46_I 50_D 1.094 0.72
73_S 348_D 1.092 0.71
182_C 216_S 1.091 0.71
282_N 289_A 1.09 0.71
359_L 365_L 1.088 0.71
210_F 348_D 1.086 0.71
285_P 358_Q 1.086 0.71
78_G 84_R 1.085 0.71
241_L 323_A 1.083 0.70
19_Q 39_L 1.081 0.70
204_P 214_L 1.077 0.70
234_L 238_I 1.076 0.70
136_C 191_I 1.069 0.69
244_H 247_E 1.068 0.69
242_V 326_F 1.065 0.69
16_R 46_I 1.065 0.69
21_L 58_I 1.062 0.68
185_F 188_A 1.058 0.68
347_N 350_E 1.054 0.67
165_A 203_V 1.05 0.67
67_T 75_R 1.049 0.67
32_T 70_Y 1.049 0.67
129_L 132_L 1.045 0.66
82_N 85_L 1.043 0.66
32_T 35_T 1.042 0.66
365_L 371_H 1.042 0.66
358_Q 362_L 1.041 0.66
132_L 194_H 1.038 0.66
411_L 416_Q 1.036 0.65
214_L 269_Q 1.032 0.65
80_V 207_E 1.032 0.65
281_D 317_E 1.031 0.65
13_A 53_E 1.031 0.65
331_M 358_Q 1.031 0.65
17_R 53_E 1.029 0.65
407_T 411_L 1.027 0.64
243_N 254_Y 1.024 0.64
145_E 148_D 1.024 0.64
373_S 376_A 1.023 0.64
24_L 58_I 1.02 0.64
268_A 343_L 1.019 0.64
266_H 318_E 1.018 0.63
296_V 316_D 1.013 0.63
347_N 352_E 1.011 0.63
17_R 40_N 1.009 0.62
295_L 323_A 1.008 0.62
159_C 327_G 1.007 0.62
36_F 47_A 1.006 0.62
284_L 317_E 1.004 0.62
346_G 350_E 1.002 0.62
185_F 189_Q 1 0.61
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3sqnA20.91551000.365Contact Map0.547
1h99A10.51411000.627Contact Map0.494
3ufeA20.255999.70.79Contact Map0.612
3rioA10.420299.70.79Contact Map0.604
3czcA10.22399.50.813Contact Map0.391
1vkrA10.220799.20.842Contact Map0.417
1tvmA10.22399.10.849Contact Map0.274
2l2qA10.199598.50.88Contact Map0.291
1e2bA10.194898.30.885Contact Map0.401
4mgeA20.187898.10.892Contact Map0.333
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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