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OPENSEQ.org
RTCB - RNA-splicing ligase RtcB
UniProt: P46850 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12939
Length: 408 (401)
Sequences: 921
Seq/Len: 2.30
RTCB
Paralog alert: 0.31 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
347_K 350_K 3.433 1.00
347_K 351_L 3.222 1.00
169_F 313_P 3.169 1.00
186_S 250_M 3.11 1.00
288_Q 293_E 3.074 1.00
64_K 292_E 3.053 1.00
23_E 26_A 2.856 1.00
82_A 322_I 2.634 1.00
324_R 395_E 2.567 1.00
39_I 43_I 2.54 1.00
26_A 49_V 2.455 1.00
346_T 350_K 2.386 1.00
193_N 197_T 2.356 1.00
182_I 254_V 2.286 1.00
45_V 49_V 2.194 1.00
30_L 45_V 2.174 1.00
107_V 254_V 2.169 1.00
190_G 193_N 2.007 0.99
315_S 318_A 1.99 0.99
343_M 348_A 1.986 0.99
89_A 174_L 1.929 0.99
103_I 170_I 1.917 0.99
174_L 403_V 1.883 0.99
180_V 403_V 1.876 0.99
383_I 387_M 1.873 0.99
227_E 231_D 1.862 0.99
354_V 373_I 1.861 0.99
303_S 308_Q 1.849 0.99
319_K 399_T 1.771 0.98
326_L 395_E 1.736 0.98
309_Y 324_R 1.729 0.97
81_N 278_N 1.682 0.97
30_L 43_I 1.673 0.97
83_L 184_L 1.673 0.97
67_I 233_L 1.669 0.97
102_A 260_S 1.668 0.97
195_I 239_A 1.651 0.96
361_T 405_C 1.649 0.96
210_T 223_M 1.634 0.96
206_E 228_Y 1.627 0.96
57_I 72_V 1.606 0.96
106_A 257_A 1.596 0.95
79_G 247_R 1.591 0.95
335_C 394_V 1.575 0.95
286_K 293_E 1.557 0.95
318_A 321_F 1.551 0.94
77_G 244_S 1.549 0.94
15_M 22_V 1.538 0.94
174_L 401_R 1.537 0.94
83_L 251_M 1.529 0.94
13_V 30_L 1.521 0.94
324_R 397_I 1.515 0.93
163_L 170_I 1.495 0.93
352_F 376_I 1.487 0.93
82_A 181_W 1.467 0.92
304_A 327_G 1.461 0.92
173_C 183_M 1.459 0.92
382_D 385_A 1.453 0.91
359_R 362_A 1.447 0.91
25_D 347_K 1.446 0.91
18_K 385_A 1.443 0.91
19_G 22_V 1.44 0.91
89_A 93_P 1.418 0.90
56_T 59_S 1.417 0.90
89_A 178_D 1.402 0.89
224_E 295_Y 1.396 0.89
100_R 404_V 1.392 0.89
225_G 230_D 1.391 0.89
236_V 284_V 1.374 0.88
32_N 216_S 1.37 0.88
321_F 383_I 1.37 0.88
226_T 229_F 1.356 0.87
54_G 219_L 1.356 0.87
84_R 179_Q 1.35 0.87
84_R 275_E 1.324 0.85
203_A 222_F 1.32 0.85
209_E 228_Y 1.302 0.84
42_H 290_F 1.3 0.84
40_F 294_I 1.298 0.84
287_E 296_V 1.287 0.83
314_G 320_S 1.279 0.83
311_I 322_I 1.276 0.82
92_L 266_V 1.274 0.82
356_D 360_A 1.266 0.82
88_T 179_Q 1.264 0.82
30_L 49_V 1.259 0.81
33_T 71_A 1.254 0.81
90_E 178_D 1.253 0.81
59_S 297_T 1.251 0.81
108_P 163_L 1.243 0.80
77_G 283_Y 1.236 0.80
182_I 258_L 1.235 0.79
56_T 297_T 1.229 0.79
317_G 320_S 1.226 0.79
352_F 378_M 1.216 0.78
170_I 254_V 1.212 0.78
33_T 43_I 1.211 0.78
206_E 210_T 1.209 0.77
33_T 55_S 1.209 0.77
103_I 254_V 1.205 0.77
280_H 283_Y 1.202 0.77
52_G 73_G 1.199 0.77
310_G 323_V 1.199 0.77
62_P 296_V 1.198 0.76
285_Q 303_S 1.197 0.76
314_G 317_G 1.194 0.76
40_F 292_E 1.192 0.76
305_R 333_C 1.179 0.75
305_R 308_Q 1.168 0.74
255_V 258_L 1.166 0.74
284_V 405_C 1.165 0.74
85_T 180_V 1.16 0.73
38_F 68_I 1.156 0.73
142_Q 145_T 1.155 0.73
107_V 250_M 1.149 0.72
229_F 302_V 1.148 0.72
256_T 388_A 1.146 0.72
38_F 65_G 1.136 0.71
100_R 104_E 1.136 0.71
50_H 73_G 1.135 0.71
250_M 254_V 1.133 0.71
206_E 209_E 1.127 0.70
341_R 345_R 1.121 0.69
184_L 233_L 1.119 0.69
155_N 194_A 1.117 0.69
336_S 390_Q 1.113 0.69
13_V 43_I 1.111 0.68
109_H 159_H 1.111 0.68
99_L 172_I 1.107 0.68
77_G 280_H 1.104 0.68
339_A 377_P 1.103 0.68
55_S 71_A 1.102 0.68
357_Q 376_I 1.096 0.67
61_I 71_A 1.092 0.67
224_E 233_L 1.091 0.66
202_L 228_Y 1.088 0.66
371_E 376_I 1.087 0.66
174_L 178_D 1.085 0.66
25_D 51_L 1.085 0.66
35_K 227_E 1.079 0.65
233_L 295_Y 1.076 0.65
353_S 356_D 1.074 0.65
201_D 205_K 1.074 0.65
107_V 364_V 1.073 0.65
24_A 28_Q 1.071 0.64
384_D 387_M 1.067 0.64
321_F 387_M 1.063 0.64
314_G 383_I 1.057 0.63
202_L 231_D 1.056 0.63
244_S 248_D 1.054 0.63
375_E 380_Y 1.054 0.63
209_E 212_E 1.048 0.62
121_G 130_V 1.047 0.62
55_S 59_S 1.046 0.62
360_A 378_M 1.031 0.60
385_A 388_A 1.029 0.60
89_A 403_V 1.029 0.60
82_A 183_M 1.029 0.60
387_M 394_V 1.027 0.60
186_S 315_S 1.027 0.60
99_L 258_L 1.027 0.60
212_E 221_Y 1.026 0.59
65_G 235_A 1.026 0.59
175_D 398_Y 1.023 0.59
26_A 30_L 1.021 0.59
133_K 136_E 1.021 0.59
286_K 295_Y 1.02 0.59
33_T 53_K 1.02 0.59
302_V 310_G 1.017 0.58
345_R 375_E 1.017 0.58
233_L 237_A 1.017 0.58
107_V 257_A 1.016 0.58
139_A 142_Q 1.015 0.58
58_G 73_G 1.013 0.58
184_L 247_R 1.01 0.58
113_T 162_T 1.008 0.57
315_S 371_E 1.006 0.57
33_T 39_I 1.005 0.57
69_P 169_F 1.003 0.57
77_G 186_S 1.003 0.57
344_S 348_A 1.001 0.57
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2epgA20.98771000.079Contact Map0.728
4dwrB30.98771000.082Contact Map0.718
1pbyA10.178919.90.978Contact Map0.229
2cosA10.115214.60.979Contact Map0.598
1w2lA10.198511.60.98Contact Map0.047
2b78A10.2997.20.982Contact Map0.281
3vseA40.30644.60.984Contact Map0.131
2vycA100.13974.60.984Contact Map0.317
1i8oA10.19854.10.984Contact Map0.114
1c75A10.159340.984Contact Map0.034
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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