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OPENSEQ.org
GSPJ - Putative type II secretion system protein J
UniProt: P45761 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12893
Length: 195 (177)
Sequences: 303
Seq/Len: 1.71
GSPJ
Paralog alert: 0.18 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
143_E 157_T 4.827 1.00
107_W 133_M 2.529 1.00
94_A 99_A 2.23 0.99
68_R 123_D 2.18 0.99
54_T 134_L 2.166 0.99
92_P 99_A 2.056 0.99
58_L 158_L 1.893 0.98
20_F 48_F 1.609 0.93
26_L 92_P 1.536 0.90
7_G 10_L 1.498 0.89
52_Q 95_P 1.497 0.88
9_T 12_E 1.489 0.88
7_G 12_E 1.468 0.87
47_Q 164_Q 1.464 0.87
157_T 169_Q 1.446 0.86
108_Y 117_A 1.441 0.86
20_F 52_Q 1.414 0.84
58_L 114_L 1.405 0.84
54_T 114_L 1.392 0.83
8_F 12_E 1.38 0.82
32_S 36_E 1.373 0.82
113_T 132_A 1.315 0.78
104_T 119_R 1.289 0.76
16_A 31_F 1.288 0.76
28_F 32_S 1.271 0.74
50_Q 135_E 1.269 0.74
27_A 160_L 1.242 0.72
65_L 156_V 1.237 0.72
132_A 135_E 1.228 0.71
124_G 172_F 1.219 0.70
94_A 98_E 1.218 0.70
99_A 155_S 1.217 0.70
23_L 165_Y 1.216 0.70
64_Q 123_D 1.215 0.70
17_L 69_R 1.21 0.69
162_T 166_G 1.209 0.69
137_V 163_Q 1.205 0.69
29_M 155_S 1.195 0.68
14_M 102_L 1.193 0.68
58_L 133_M 1.192 0.68
106_H 119_R 1.186 0.67
14_M 21_S 1.184 0.67
59_D 172_F 1.174 0.66
80_G 144_S 1.17 0.65
58_L 62_L 1.156 0.64
65_L 155_S 1.155 0.64
138_E 161_Q 1.151 0.64
107_W 168_L 1.15 0.63
6_Q 51_L 1.135 0.62
105_V 116_R 1.134 0.62
19_I 32_S 1.125 0.61
13_V 112_H 1.124 0.61
136_H 150_Q 1.12 0.60
94_A 103_L 1.108 0.59
71_R 127_D 1.104 0.59
25_V 167_G 1.101 0.58
53_R 143_E 1.094 0.58
13_V 53_R 1.086 0.57
79_L 156_V 1.08 0.56
17_L 105_V 1.071 0.55
45_I 161_Q 1.07 0.55
156_V 167_G 1.069 0.55
15_A 67_A 1.069 0.55
23_L 47_Q 1.068 0.55
159_H 167_G 1.068 0.55
103_L 118_V 1.064 0.55
23_L 30_I 1.062 0.54
38_H 59_D 1.061 0.54
55_I 170_R 1.059 0.54
121_S 127_D 1.058 0.54
95_P 99_A 1.057 0.54
46_Q 55_I 1.056 0.54
48_F 65_L 1.054 0.53
21_S 98_E 1.048 0.53
61_D 116_R 1.046 0.53
17_L 24_S 1.045 0.53
55_I 172_F 1.045 0.53
108_Y 158_L 1.043 0.52
36_E 156_V 1.038 0.52
7_G 11_L 1.038 0.52
6_Q 113_T 1.037 0.52
136_H 163_Q 1.036 0.52
42_Q 171_R 1.032 0.51
86_T 89_S 1.031 0.51
59_D 170_R 1.027 0.51
18_A 22_M 1.026 0.51
6_Q 149_S 1.02 0.50
22_M 58_L 1.017 0.50
139_S 161_Q 1.016 0.49
77_M 83_A 1.01 0.49
13_V 97_S 1.007 0.48
68_R 144_S 1.007 0.48
104_T 155_S 1.006 0.48
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3njeA20.86151000.43Contact Map0.543
3ci0J10.72311000.479Contact Map0.554
2retB40.77951000.487Contact Map0.6
2m7gA10.312897.70.858Contact Map0.089
3sokA20.671895.40.893Contact Map0.208
2hi2A10.728295.20.894Contact Map0.17
1oqwA20.6051950.895Contact Map0.221
3pjsK40.343610.80.955Contact Map0.316
3effK40.34368.70.957Contact Map0.322
4h33A10.35388.10.957Contact Map0.355
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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