OPENSEQ.org - GSPG - ECOLI GREMLIN

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GSPG - Putative type II secretion system protein G

UniProt:	P41442	Sequence MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12886
Length:	145 (136)
Sequences:	673
Seq/Len:	4.95

GSPG
Paralog alert: 0.29 [within 20: 0.01] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
57_D	96_K	3.28	1.00
45_A	102_P	2.673	1.00
59_Y	71_Q	2.611	1.00
126_D	134_D	2.439	1.00
74_E	78_E	2.409	1.00
53_E	137_N	2.22	1.00
125_P	131_T	2.216	1.00
100_A	106_D	2.16	1.00
108_V	121_L	1.994	1.00
123_A	131_T	1.699	1.00
16_M	19_I	1.663	0.99
112_P	119_D	1.653	0.99
36_N	39_K	1.635	0.99
26_A	30_V	1.535	0.99
59_Y	65_H	1.51	0.99
69_T	111_N	1.498	0.98
108_V	123_A	1.449	0.98
121_L	136_T	1.445	0.98
65_H	71_Q	1.4	0.97
119_D	138_W	1.388	0.97
62_D	94_Y	1.316	0.96
45_A	107_Y	1.297	0.95
56_L	59_Y	1.295	0.95
49_I	120_L	1.281	0.95
50_V	53_E	1.274	0.95
45_A	49_I	1.273	0.95
53_E	120_L	1.256	0.94
102_P	107_Y	1.249	0.94
115_H	138_W	1.223	0.93
49_I	137_N	1.203	0.92
19_I	37_K	1.194	0.92
115_H	137_N	1.179	0.91
79_A	86_A	1.176	0.91
112_P	116_G	1.169	0.91
11_T	14_E	1.148	0.90
8_R	129_M	1.148	0.90
15_I	25_L	1.144	0.89
41_D	47_S	1.144	0.89
11_T	17_V	1.144	0.89
44_K	48_D	1.142	0.89
113_G	116_G	1.128	0.89
113_G	119_D	1.124	0.88
27_S	43_Q	1.12	0.88
110_V	114_E	1.116	0.88
37_K	41_D	1.114	0.88
72_G	75_S	1.11	0.87
24_V	47_S	1.099	0.87
34_M	37_K	1.097	0.87
56_L	120_L	1.095	0.86
9_G	12_L	1.068	0.85
52_L	95_I	1.06	0.84
105_N	127_G	1.052	0.83
113_G	138_W	1.05	0.83
55_A	62_D	1.043	0.83
115_H	119_D	1.039	0.82
16_M	30_V	1.034	0.82
20_V	23_G	1.011	0.80
27_S	31_P	1.009	0.80
15_I	139_G	1.008	0.80
89_Y	93_G	1.008	0.80
76_L	101_D	1.005	0.79

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
3g20A	2	0.8483	100	0.38	Contact Map	0.666
3gn9A	3	0.7724	99.9	0.44	Contact Map	0.707
2kepA	1	0.7379	99.9	0.449	Contact Map	0.525
1t92A	2	0.7724	99.9	0.449	Contact Map	0.466
2hi2A	1	0.8552	99.8	0.542	Contact Map	0.287
3sokA	2	0.8828	99.8	0.565	Contact Map	0.316
2m7gA	1	0.4207	99.6	0.645	Contact Map	0.295
1oqwA	2	0.8276	99.3	0.69	Contact Map	0.425
2m3kA	1	0.7034	99.2	0.708	Contact Map	0.232
3jyzA	1	0.731	65.3	0.91	Contact Map	0.273

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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