OPENSEQ.org - SPRT - ECOLI GREMLIN

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SPRT - Protein SprT

UniProt:	P39902	Sequence MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRGTSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWMMENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGEQLVAK	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12122
Length:	165 (159)
Sequences:	180
Seq/Len:	1.13

SPRT
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
18_L	80_L	1.919	0.95
66_E	121_S	1.853	0.94
26_N	33_Y	1.82	0.93
139_T	148_R	1.816	0.93
55_Y	87_K	1.805	0.93
75_V	117_F	1.799	0.93
46_S	49_T	1.774	0.92
24_Q	27_L	1.724	0.90
104_E	109_V	1.683	0.89
42_Q	49_T	1.653	0.88
29_L	33_Y	1.631	0.87
132_K	160_E	1.597	0.85
17_R	139_T	1.586	0.85
85_V	136_H	1.572	0.84
49_T	114_T	1.559	0.83
58_R	61_P	1.544	0.82
114_T	154_R	1.541	0.82
119_L	151_A	1.511	0.81
31_R	88_H	1.462	0.77
104_E	111_A	1.418	0.74
23_A	68_S	1.413	0.74
140_V	144_N	1.408	0.74
67_N	71_F	1.391	0.72
18_L	57_I	1.388	0.72
22_L	80_L	1.379	0.71
38_L	76_V	1.364	0.70
69_E	73_E	1.363	0.70
45_T	113_R	1.343	0.68
41_T	118_E	1.333	0.68
100_K	104_E	1.328	0.67
53_E	125_N	1.305	0.65
40_Y	80_L	1.301	0.65
63_L	67_N	1.297	0.65
14_V	76_V	1.294	0.64
22_L	40_Y	1.293	0.64
81_A	136_H	1.293	0.64
49_T	145_R	1.29	0.64
81_A	102_M	1.275	0.63
51_W	145_R	1.263	0.62
22_L	57_I	1.252	0.61
89_F	101_W	1.241	0.59
36_P	50_A	1.221	0.58
84_L	102_M	1.22	0.58
140_V	145_R	1.207	0.56
50_A	71_F	1.201	0.56
64_L	80_L	1.175	0.53
138_L	144_N	1.172	0.53
17_R	76_V	1.166	0.52
74_E	112_R	1.154	0.51
22_L	64_L	1.14	0.50
128_P	140_V	1.137	0.50
26_N	35_E	1.13	0.49
85_V	93_A	1.128	0.49
58_R	106_V	1.108	0.47
127_F	144_N	1.106	0.47
10_I	14_V	1.104	0.46
134_Q	157_H	1.104	0.46
16_R	20_E	1.097	0.46
67_N	83_L	1.095	0.46
51_W	87_K	1.094	0.46
34_P	147_V	1.085	0.45
51_W	57_I	1.084	0.45
68_S	135_E	1.084	0.45
146_V	161_Q	1.069	0.43
26_N	31_R	1.067	0.43
113_R	124_R	1.065	0.43
18_L	59_L	1.065	0.43
55_Y	83_L	1.056	0.42
84_L	114_T	1.053	0.42
26_N	32_N	1.052	0.42
50_A	67_N	1.042	0.41
40_Y	57_I	1.04	0.40
88_H	134_Q	1.034	0.40
18_L	40_Y	1.033	0.40
36_P	67_N	1.031	0.40
81_A	134_Q	1.026	0.39
66_E	83_L	1.025	0.39
16_R	40_Y	1.023	0.39
38_L	147_V	1.023	0.39
41_T	161_Q	1.02	0.39
64_L	84_L	1.018	0.38
97_K	111_A	1.016	0.38
87_K	100_K	1.011	0.38
8_I	13_A	1.01	0.38
91_R	98_E	1.003	0.37
46_S	64_L	1.002	0.37
144_N	148_R	1.002	0.37
46_S	114_T	1.001	0.37

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
4jixA	2	0.6788	99.7	0.764	Contact Map	0.34
4jiuA	1	0.6364	98.5	0.864	Contact Map	0.306
1bboA	1	0.2303	81.7	0.939	Contact Map	0.14
2cshA	1	0.2848	79.1	0.94	Contact Map	0.116
3cqbA	2	0.5333	77.5	0.941	Contact Map	0.372
2lv2A	1	0.3939	77.1	0.941	Contact Map	0.135
2lceA	1	0.3273	74.7	0.943	Contact Map	0.026
2kmkA	1	0.3939	74.6	0.943	Contact Map	0.128
1f2iG	6	0.3394	73.7	0.943	Contact Map	0.244
2adrA	1	0.2364	73.5	0.943	Contact Map	0.243

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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