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OPENSEQ.org
RLMA - 23S rRNA (guanine(745)-N(1))-methyltransferase
UniProt: P36999 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12207
Length: 269 (268)
Sequences: 593
Seq/Len: 2.21
RLMA
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
60_A 229_A 3.893 1.00
186_E 257_Q 3.867 1.00
150_D 169_K 2.781 1.00
73_A 214_A 2.471 1.00
90_L 149_M 2.325 1.00
142_L 167_V 2.292 1.00
175_I 262_I 2.271 1.00
122_I 125_A 2.237 1.00
92_I 164_L 2.162 1.00
5_C 8_C 2.088 0.99
19_Y 35_V 2.082 0.99
70_L 260_F 1.867 0.99
182_R 217_C 1.809 0.98
163_E 166_R 1.77 0.98
219_P 255_D 1.719 0.97
69_P 218_Y 1.651 0.96
186_E 256_C 1.65 0.96
103_F 153_I 1.645 0.96
215_E 259_D 1.598 0.95
94_C 114_G 1.593 0.95
175_I 264_L 1.565 0.94
74_I 175_I 1.563 0.94
223_R 226_E 1.552 0.94
7_L 39_P 1.543 0.94
158_P 179_P 1.537 0.94
140_H 160_K 1.535 0.94
54_M 118_S 1.53 0.93
74_I 177_A 1.505 0.93
99_Y 183_H 1.493 0.92
236_F 260_F 1.475 0.92
68_Q 72_D 1.467 0.91
90_L 144_F 1.452 0.91
92_I 139_S 1.452 0.91
154_R 164_L 1.437 0.90
18_S 26_Q 1.428 0.90
158_P 163_E 1.407 0.89
221_R 255_D 1.384 0.88
78_L 151_A 1.376 0.87
142_L 163_E 1.375 0.87
180_G 259_D 1.366 0.87
173_W 264_L 1.365 0.87
62_L 98_Y 1.353 0.86
66_H 226_E 1.347 0.86
4_S 145_S 1.332 0.85
182_R 257_Q 1.323 0.84
54_M 156_Y 1.321 0.84
32_E 123_K 1.305 0.83
70_L 262_I 1.281 0.82
64_A 226_E 1.27 0.81
67_Y 99_Y 1.265 0.81
27_F 216_L 1.258 0.80
45_S 48_P 1.255 0.80
55_M 156_Y 1.25 0.80
170_P 181_P 1.25 0.80
73_A 76_A 1.242 0.79
20_I 174_V 1.239 0.79
193_N 196_H 1.218 0.77
29_M 35_V 1.204 0.76
182_R 186_E 1.2 0.76
218_Y 258_T 1.191 0.75
121_A 222_L 1.185 0.74
71_R 99_Y 1.183 0.74
11_P 38_L 1.18 0.74
5_C 21_C 1.169 0.73
6_P 12_L 1.166 0.73
216_L 262_I 1.163 0.72
62_L 99_Y 1.158 0.72
62_L 183_H 1.157 0.72
168_V 172_G 1.145 0.71
256_C 259_D 1.142 0.70
37_L 49_G 1.14 0.70
211_Q 266_Q 1.138 0.70
18_S 29_M 1.137 0.70
178_T 258_T 1.134 0.70
50_D 55_M 1.131 0.69
100_T 153_I 1.129 0.69
185_M 257_Q 1.123 0.69
165_A 208_F 1.12 0.68
45_S 49_G 1.12 0.68
149_M 167_V 1.11 0.67
66_H 222_L 1.109 0.67
100_T 159_C 1.108 0.67
154_R 157_A 1.107 0.67
155_I 158_P 1.1 0.66
100_T 112_T 1.1 0.66
156_Y 183_H 1.1 0.66
10_Q 13_S 1.097 0.66
4_S 186_E 1.088 0.65
89_V 169_K 1.086 0.65
48_P 117_V 1.084 0.65
114_G 192_Y 1.084 0.65
25_H 28_D 1.083 0.64
3_F 135_C 1.082 0.64
75_V 110_I 1.081 0.64
90_L 197_L 1.066 0.63
71_R 102_A 1.064 0.62
62_L 118_S 1.06 0.62
94_C 134_F 1.055 0.61
247_T 251_K 1.051 0.61
91_D 96_E 1.05 0.61
184_L 230_L 1.046 0.60
61_F 67_Y 1.045 0.60
202_A 206_E 1.043 0.60
135_C 144_F 1.039 0.60
21_C 25_H 1.039 0.60
157_A 178_T 1.035 0.59
62_L 65_G 1.035 0.59
40_V 48_P 1.032 0.59
43_K 48_P 1.029 0.59
152_I 267_R 1.028 0.58
209_T 266_Q 1.028 0.58
182_R 185_M 1.026 0.58
180_G 261_N 1.024 0.58
47_D 118_S 1.023 0.58
19_Y 29_M 1.022 0.58
91_D 94_C 1.022 0.58
10_Q 22_P 1.02 0.58
61_F 66_H 1.02 0.58
62_L 156_Y 1.016 0.57
49_G 195_V 1.015 0.57
7_L 27_F 1.013 0.57
54_M 121_A 1.005 0.56
55_M 144_F 1.003 0.56
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1p91A211000.443Contact Map0.618
2yqzA20.780799.90.731Contact Map0.545
4e2xA10.929499.90.734Contact Map0.345
4kriA30.977799.90.748Contact Map0.403
3ccfA20.869999.90.75Contact Map0.59
2p35A20.780799.90.751Contact Map0.506
4hg2A20.799399.90.751Contact Map0.545
3ujcA10.869999.80.769Contact Map0.526
3l8dA10.765899.80.77Contact Map0.677
3b5iA20.795599.80.773Contact Map0.545
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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