OPENSEQ.org - ARFA - ECOLI GREMLIN

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ARFA - Alternative ribosome-rescue factor A

UniProt:	P36675	Sequence MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGKHGNRGNWEASGKKVNHFFTTGLLLSGAC	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG11968
Length:	72 (69)
Sequences:	74
Seq/Len:	1.07

ARFA
Paralog alert: 0.00 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
62_F	65_G	2.13	0.98
21_H	27_Q	1.778	0.91
2_S	10_Q	1.758	0.91
16_I	43_G	1.714	0.89
12_K	33_K	1.702	0.89
47_N	51_W	1.575	0.83
43_G	50_N	1.49	0.78
14_N	33_K	1.483	0.77
5_Q	66_L	1.385	0.70
10_Q	48_R	1.378	0.70
44_K	52_E	1.345	0.67
40_M	43_G	1.287	0.62
32_N	55_G	1.286	0.62
4_Y	52_E	1.279	0.61
4_Y	22_D	1.262	0.60
12_K	61_F	1.237	0.57
17_E	20_L	1.233	0.57
16_I	49_G	1.226	0.56
16_I	48_R	1.192	0.53
6_H	19_L	1.169	0.51
31_K	40_M	1.159	0.50
22_D	52_E	1.149	0.49
29_V	46_G	1.124	0.47
23_P	44_K	1.112	0.46
8_K	31_K	1.09	0.44
64_T	67_L	1.09	0.44
2_S	33_K	1.077	0.42
8_K	46_G	1.072	0.42
2_S	29_V	1.067	0.42
55_G	60_H	1.062	0.41
3_R	10_Q	1.06	0.41
6_H	52_E	1.048	0.40
46_G	61_F	1.041	0.39
14_N	42_K	1.04	0.39
11_I	23_P	1.026	0.38
1_M	54_S	1.012	0.37
11_I	45_H	1.009	0.36
4_Y	44_K	1.001	0.36
26_R	31_K	1	0.36

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results:

PDB	Chains	Cov	P(%)	HHΔ		Acc
2q8kA	1	0.75	14.8	0.919	Contact Map	0.212
4ipaA	4	0.7361	8.1	0.928	Contact Map	0.209
3jtnA	2	0.3333	5.6	0.934	Contact Map	0.384
1ekrA	1	0.5139	5.2	0.934	Contact Map	0.01
2qguA	1	0.5	3.8	0.938	Contact Map	0.259
1baxA	1	0.3333	3.6	0.94	Contact Map	0.011
4kisA	4	0.8056	3.3	0.941	Contact Map	0
2wmfA	1	0.8194	3.2	0.941	Contact Map	0.267
2yx6A	4	0.4444	2.7	0.943	Contact Map	0.155
4bqqA	2	0.7361	2.7	0.943	Contact Map	0

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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