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OPENSEQ.org
DSBD - Thiol:disulfide interchange protein DsbD
UniProt: P36655 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12178
Length: 565 (538)
Sequences: 693
Seq/Len: 1.29
DSBD
Paralog alert: 0.05 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
536_D 540_Q 3.402 1.00
34_D 134_K 2.854 1.00
75_A 105_T 2.663 1.00
172_L 176_G 2.516 1.00
214_V 337_G 2.348 0.99
86_E 92_K 2.317 0.99
374_V 389_L 2.206 0.99
62_R 82_G 2.199 0.99
171_L 338_L 2.196 0.99
180_T 183_V 2.194 0.99
30_F 127_F 2.152 0.98
216_G 291_F 2.097 0.98
215_Q 330_Y 2.095 0.98
48_L 115_L 2.059 0.98
120_Q 129_Y 2.05 0.98
490_T 550_F 2.046 0.98
472_M 534_F 2.014 0.97
526_V 531_T 1.986 0.97
222_T 333_A 1.982 0.97
229_A 317_A 1.961 0.97
452_Q 507_Q 1.96 0.97
541_E 546_R 1.945 0.97
422_A 433_D 1.926 0.96
469_K 504_V 1.914 0.96
86_E 91_G 1.889 0.96
552_D 555_T 1.869 0.95
59_Y 85_H 1.867 0.95
30_F 125_A 1.838 0.95
62_R 94_E 1.835 0.95
257_M 262_L 1.829 0.95
82_G 94_E 1.828 0.95
207_L 345_V 1.809 0.94
83_V 97_R 1.792 0.94
460_N 523_H 1.775 0.94
97_R 124_D 1.762 0.93
177_I 332_Y 1.737 0.93
266_Q 272_Q 1.734 0.93
456_V 465_E 1.705 0.92
254_L 263_F 1.693 0.91
236_Q 379_R 1.69 0.91
267_L 271_L 1.682 0.91
485_E 550_F 1.68 0.91
184_L 333_A 1.605 0.88
203_T 206_A 1.601 0.88
499_A 561_R 1.601 0.88
256_A 260_F 1.596 0.88
426_V 429_R 1.587 0.87
458_E 462_A 1.543 0.85
426_V 433_D 1.5 0.83
256_A 263_F 1.493 0.83
493_D 496_V 1.491 0.82
54_I 99_R 1.488 0.82
510_V 517_D 1.478 0.82
521_L 526_V 1.463 0.81
429_R 433_D 1.447 0.80
246_I 250_I 1.447 0.80
456_V 523_H 1.446 0.80
371_A 374_V 1.441 0.79
87_D 90_Y 1.427 0.78
84_W 92_K 1.422 0.78
206_A 345_V 1.414 0.78
59_Y 87_D 1.399 0.77
243_Y 246_I 1.396 0.76
39_F 136_V 1.384 0.75
179_F 226_L 1.379 0.75
228_V 302_S 1.377 0.75
306_T 317_A 1.376 0.75
120_Q 126_G 1.37 0.74
170_A 312_I 1.358 0.74
251_V 271_L 1.348 0.73
32_P 35_Q 1.345 0.72
462_A 506_L 1.341 0.72
307_A 311_A 1.339 0.72
471_V 504_V 1.338 0.72
126_G 129_Y 1.336 0.72
549_G 556_F 1.326 0.71
473_L 506_L 1.323 0.71
256_A 370_L 1.323 0.71
485_E 556_F 1.321 0.71
31_V 36_A 1.306 0.69
87_D 130_P 1.297 0.69
74_I 464_V 1.293 0.68
229_A 322_M 1.29 0.68
411_G 414_R 1.287 0.68
554_E 558_A 1.285 0.67
485_E 490_T 1.281 0.67
393_L 402_F 1.277 0.67
451_T 462_A 1.277 0.67
75_A 106_I 1.276 0.67
367_F 395_V 1.275 0.67
418_I 424_A 1.273 0.66
165_F 170_A 1.272 0.66
235_F 313_L 1.271 0.66
194_V 367_F 1.267 0.66
454_K 514_D 1.265 0.66
400_W 403_I 1.265 0.66
542_H 545_A 1.265 0.66
269_S 273_T 1.262 0.65
342_L 438_A 1.253 0.65
48_L 104_V 1.251 0.64
333_A 348_N 1.25 0.64
210_T 344_T 1.248 0.64
209_L 213_Y 1.239 0.63
39_F 52_W 1.235 0.63
85_H 97_R 1.235 0.63
30_F 33_A 1.233 0.63
65_I 258_S 1.233 0.63
192_G 251_V 1.232 0.63
189_L 297_A 1.227 0.62
265_L 269_S 1.219 0.61
429_R 434_W 1.216 0.61
508_A 520_L 1.209 0.61
305_T 529_L 1.204 0.60
221_Y 298_G 1.203 0.60
287_P 418_I 1.202 0.60
302_S 306_T 1.202 0.60
181_P 184_L 1.201 0.60
121_G 129_Y 1.199 0.60
176_G 361_V 1.196 0.59
367_F 397_F 1.191 0.59
85_H 95_I 1.191 0.59
54_I 418_I 1.19 0.59
396_A 402_F 1.186 0.58
378_E 559_H 1.185 0.58
328_T 475_L 1.183 0.58
82_G 87_D 1.173 0.57
510_V 520_L 1.171 0.57
34_D 119_Y 1.165 0.56
310_S 373_P 1.164 0.56
216_G 234_Q 1.164 0.56
194_V 344_T 1.163 0.56
190_I 210_T 1.162 0.56
39_F 50_L 1.161 0.56
302_S 376_L 1.158 0.56
426_V 434_W 1.153 0.55
229_A 316_I 1.149 0.55
394_G 397_F 1.146 0.54
524_L 535_F 1.142 0.54
263_F 346_F 1.141 0.54
261_G 264_T 1.141 0.54
187_Y 224_L 1.14 0.54
50_L 136_V 1.14 0.54
60_L 342_L 1.138 0.54
123_A 126_G 1.138 0.54
422_A 426_V 1.137 0.54
56_D 198_K 1.136 0.53
300_I 316_I 1.132 0.53
207_L 211_F 1.128 0.53
359_E 363_T 1.126 0.52
454_K 517_D 1.126 0.52
456_V 460_N 1.126 0.52
493_D 536_D 1.118 0.52
354_S 372_L 1.115 0.51
240_Q 479_W 1.11 0.51
115_L 461_Q 1.109 0.51
231_A 234_Q 1.108 0.51
316_I 321_N 1.106 0.50
184_L 187_Y 1.106 0.50
177_I 190_I 1.104 0.50
221_Y 485_E 1.102 0.50
466_A 500_L 1.101 0.50
499_A 557_S 1.099 0.50
235_F 252_F 1.099 0.50
230_A 317_A 1.099 0.50
358_M 505_L 1.098 0.50
123_A 129_Y 1.096 0.49
301_C 529_L 1.094 0.49
31_V 58_Y 1.093 0.49
117_V 353_K 1.091 0.49
482_A 486_F 1.09 0.49
173_I 176_G 1.088 0.49
263_F 347_G 1.085 0.48
422_A 434_W 1.085 0.48
427_S 434_W 1.085 0.48
282_Q 286_S 1.083 0.48
396_A 432_Q 1.082 0.48
284_G 468_G 1.08 0.48
255_L 365_F 1.078 0.48
181_P 307_A 1.075 0.47
551_M 555_T 1.072 0.47
301_C 305_T 1.072 0.47
291_F 333_A 1.071 0.47
487_E 507_Q 1.069 0.47
450_F 453_I 1.068 0.47
31_V 124_D 1.067 0.47
276_T 300_I 1.067 0.47
50_L 104_V 1.067 0.47
450_F 505_L 1.064 0.46
168_L 265_L 1.064 0.46
154_P 558_A 1.063 0.46
220_T 295_A 1.061 0.46
217_M 336_M 1.059 0.46
149_Q 444_T 1.059 0.46
337_G 370_L 1.058 0.46
485_E 489_Y 1.057 0.46
44_N 47_D 1.056 0.46
52_W 60_L 1.056 0.46
248_L 307_A 1.055 0.45
205_R 274_R 1.055 0.45
491_F 556_F 1.055 0.45
95_I 100_L 1.055 0.45
60_L 117_V 1.054 0.45
183_V 305_T 1.052 0.45
125_A 378_E 1.051 0.45
255_L 263_F 1.051 0.45
227_V 246_I 1.048 0.45
195_L 521_L 1.045 0.44
170_A 297_A 1.044 0.44
290_V 293_M 1.044 0.44
35_Q 40_D 1.043 0.44
555_T 558_A 1.042 0.44
61_Y 87_D 1.037 0.44
167_A 171_L 1.037 0.44
224_L 314_L 1.036 0.44
119_Y 134_K 1.035 0.43
231_A 306_T 1.033 0.43
315_Y 321_N 1.033 0.43
168_L 172_L 1.033 0.43
274_R 371_A 1.032 0.43
30_F 123_A 1.032 0.43
425_L 433_D 1.031 0.43
495_Q 554_E 1.029 0.43
489_Y 553_A 1.028 0.43
298_G 302_S 1.027 0.43
58_Y 307_A 1.026 0.43
346_F 368_V 1.026 0.43
213_Y 290_V 1.026 0.43
399_G 403_I 1.026 0.43
270_S 273_T 1.026 0.43
466_A 470_P 1.025 0.42
330_Y 334_L 1.025 0.42
498_K 501_A 1.024 0.42
379_R 386_G 1.024 0.42
482_A 529_L 1.023 0.42
251_V 254_L 1.022 0.42
472_M 532_I 1.022 0.42
226_L 316_I 1.02 0.42
176_G 291_F 1.02 0.42
472_M 505_L 1.019 0.42
490_T 553_A 1.019 0.42
33_A 302_S 1.018 0.42
218_A 315_Y 1.015 0.42
178_A 369_I 1.014 0.41
179_F 330_Y 1.014 0.41
298_G 312_I 1.012 0.41
495_Q 557_S 1.011 0.41
476_Y 507_Q 1.01 0.41
253_T 370_L 1.01 0.41
344_T 348_N 1.01 0.41
189_L 344_T 1.009 0.41
239_L 520_L 1.009 0.41
547_V 551_M 1.008 0.41
268_P 366_G 1.008 0.41
74_I 353_K 1.007 0.41
503_T 547_V 1.006 0.41
246_I 377_L 1.004 0.40
497_Q 500_L 1.004 0.40
298_G 529_L 1.004 0.40
309_L 329_L 1.004 0.40
288_G 353_K 1.002 0.40
52_W 123_A 1.002 0.40
233_L 317_A 1.001 0.40
258_S 263_F 1 0.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3pfuA10.2461000.814Contact Map0.66
1l6pA10.22121000.815Contact Map0.636
1jpeA10.24961000.819Contact Map0.643
2k0rA10.22121000.82Contact Map0.401
2ec4A10.261999.90.871Contact Map0.38
2fwhA10.226599.80.885Contact Map0.665
2dlxA10.224899.80.89Contact Map0.46
3f9uA20.244299.70.9Contact Map0.564
3fk8A10.210699.70.902Contact Map0.547
2kucA10.208899.70.903Contact Map0.588
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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