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OPENSEQ.org
OSMF - Putative osmoprotectant uptake system substrate-binding protein OsmF
UniProt: P33362 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12012
Length: 305 (284)
Sequences: 997
Seq/Len: 3.51
OSMF
Paralog alert: 0.33 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
282_S 288_L 4.563 1.00
148_A 236_P 4.476 1.00
272_D 275_T 4.185 1.00
136_Q 229_G 3.758 1.00
134_V 144_L 3.752 1.00
145_T 231_Q 3.401 1.00
295_A 299_K 3.105 1.00
138_V 142_N 3.012 1.00
275_T 279_L 2.83 1.00
136_Q 231_Q 2.797 1.00
282_S 296_D 2.758 1.00
119_I 251_E 2.715 1.00
163_A 213_V 2.657 1.00
136_Q 140_E 2.411 1.00
133_A 218_A 2.39 1.00
118_L 248_V 2.356 1.00
42_N 46_Q 2.143 1.00
213_V 216_A 2.118 1.00
251_E 255_R 2.069 1.00
275_T 301_K 2.023 1.00
270_S 301_K 2 1.00
31_S 41_G 1.974 1.00
117_K 251_E 1.91 1.00
47_V 263_W 1.834 0.99
283_I 293_V 1.815 0.99
30_G 76_L 1.812 0.99
102_Q 106_E 1.801 0.99
179_E 185_K 1.757 0.99
122_T 265_Q 1.751 0.99
226_A 278_Q 1.74 0.99
177_A 239_V 1.714 0.99
45_L 57_N 1.709 0.99
69_G 72_T 1.695 0.99
39_L 283_I 1.682 0.99
274_K 277_Q 1.65 0.99
131_T 232_T 1.62 0.98
47_V 267_V 1.618 0.98
27_V 53_V 1.616 0.98
160_F 215_A 1.598 0.98
26_P 54_P 1.583 0.98
146_S 234_S 1.583 0.98
134_V 138_V 1.57 0.98
282_S 286_E 1.564 0.98
46_Q 295_A 1.562 0.98
271_L 279_L 1.507 0.97
120_W 246_A 1.486 0.97
296_D 300_Q 1.472 0.96
68_R 116_N 1.46 0.96
145_T 234_S 1.459 0.96
51_H 266_P 1.443 0.96
28_K 56_V 1.443 0.96
165_S 219_Y 1.44 0.96
44_I 80_P 1.387 0.94
49_E 57_N 1.38 0.94
132_I 215_A 1.375 0.94
113_S 251_E 1.367 0.94
42_N 57_N 1.353 0.93
105_Y 109_K 1.347 0.93
113_S 119_I 1.345 0.93
126_A 276_L 1.344 0.93
126_A 245_P 1.341 0.93
129_T 220_G 1.328 0.93
26_P 56_V 1.323 0.92
68_R 112_D 1.306 0.92
32_K 79_Y 1.296 0.91
146_S 236_P 1.292 0.91
86_G 108_V 1.285 0.91
291_K 295_A 1.282 0.91
127_N 273_A 1.281 0.91
142_N 153_Y 1.28 0.91
47_V 303_W 1.273 0.90
144_L 153_Y 1.272 0.90
43_I 267_V 1.255 0.89
43_I 297_Y 1.232 0.88
100_N 103_Q 1.221 0.88
278_Q 282_S 1.22 0.88
106_E 110_K 1.219 0.88
110_K 114_E 1.198 0.86
288_L 296_D 1.186 0.86
288_L 292_K 1.184 0.85
254_L 260_M 1.174 0.85
296_D 299_K 1.165 0.84
253_V 257_Y 1.152 0.83
33_I 60_Q 1.148 0.83
31_S 57_N 1.148 0.83
140_E 234_S 1.144 0.83
51_H 263_W 1.144 0.83
289_D 292_K 1.137 0.82
134_V 142_N 1.132 0.82
224_P 284_A 1.132 0.82
44_I 247_P 1.131 0.82
265_Q 268_F 1.124 0.81
46_Q 291_K 1.123 0.81
135_R 214_N 1.121 0.81
43_I 47_V 1.12 0.81
68_R 72_T 1.114 0.80
270_S 297_Y 1.108 0.80
33_I 219_Y 1.101 0.79
218_A 232_T 1.099 0.79
35_T 284_A 1.094 0.79
119_I 261_A 1.089 0.78
36_E 221_T 1.082 0.78
135_R 211_S 1.08 0.77
191_L 194_L 1.077 0.77
131_T 147_L 1.077 0.77
290_A 294_A 1.074 0.77
43_I 304_T 1.068 0.76
250_R 253_V 1.067 0.76
86_G 120_W 1.065 0.76
77_D 250_R 1.065 0.76
138_V 141_K 1.064 0.76
165_S 204_K 1.062 0.76
44_I 264_L 1.061 0.76
140_E 159_T 1.057 0.75
34_D 167_E 1.055 0.75
131_T 220_G 1.054 0.75
218_A 225_V 1.052 0.75
141_K 272_D 1.051 0.75
206_A 210_T 1.041 0.74
225_V 232_T 1.035 0.73
34_D 82_Y 1.03 0.73
237_Q 305_K 1.027 0.72
38_A 60_Q 1.024 0.72
130_W 165_S 1.023 0.72
71_I 250_R 1.017 0.71
167_E 171_R 1.015 0.71
226_A 229_G 1.008 0.70
117_K 143_K 1.005 0.70
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4ne4A10.93771000.41Contact Map0.752
3o66A20.90821000.421Contact Map0.76
3r6uA10.9181000.469Contact Map0.717
1sw5A40.90161000.507Contact Map0.8
1r9lA10.85571000.608Contact Map0.542
2rinA20.82951000.682Contact Map0.589
3l6gA10.80661000.725Contact Map0.614
3tmgA40.84921000.729Contact Map0.573
2b4lA10.491899.90.746Contact Map0.399
3un6A10.865699.30.864Contact Map0.451
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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