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OPENSEQ.org
FLGK - Flagellar hook-associated protein 1
UniProt: P33235 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11967
Length: 547 (547)
Sequences: 953
Seq/Len: 1.74
FLGK
Paralog alert: 0.06 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
11_G 529_N 4.223 1.00
24_N 197_N 3.98 1.00
195_P 199_L 3.809 1.00
162_I 280_L 3.599 1.00
187_T 199_L 3.516 1.00
206_V 225_Y 3.217 1.00
65_R 203_D 3.165 1.00
14_A 58_V 3.039 1.00
41_M 58_V 3.018 1.00
169_I 280_L 3.003 1.00
169_I 209_L 2.86 1.00
313_H 327_F 2.858 1.00
314_K 326_D 2.855 1.00
201_Q 204_Q 2.762 1.00
159_N 287_R 2.628 1.00
429_D 432_K 2.622 1.00
206_V 227_I 2.607 1.00
120_L 133_L 2.586 1.00
42_A 61_S 2.429 1.00
513_D 526_Y 2.325 1.00
26_I 512_L 2.296 1.00
518_N 521_R 2.262 1.00
309_F 452_L 2.256 1.00
21_T 39_T 2.227 0.99
95_I 151_L 2.216 0.99
22_A 518_N 2.2 0.99
117_L 309_F 2.148 0.99
172_Y 205_L 2.122 0.99
168_Q 208_E 2.092 0.99
148_D 295_R 2.066 0.99
176_I 206_V 2.058 0.99
117_L 452_L 2.05 0.99
18_A 41_M 2.039 0.99
318_D 322_D 2.034 0.99
120_L 435_M 1.973 0.98
84_G 282_G 1.967 0.98
204_Q 208_E 1.962 0.98
117_L 133_L 1.954 0.98
8_A 533_L 1.937 0.98
173_A 229_M 1.935 0.98
113_F 137_S 1.921 0.98
169_I 173_A 1.908 0.98
435_M 449_G 1.882 0.98
19_L 519_L 1.876 0.98
217_V 225_Y 1.869 0.98
155_D 295_R 1.857 0.97
537_N 541_D 1.815 0.97
24_N 37_Q 1.809 0.97
173_A 236_V 1.809 0.97
299_G 302_A 1.795 0.97
38_T 66_E 1.762 0.96
177_A 241_A 1.761 0.96
87_A 282_G 1.752 0.96
14_A 41_M 1.747 0.96
206_V 215_V 1.732 0.96
173_A 241_A 1.729 0.96
75_L 497_V 1.707 0.95
177_A 236_V 1.692 0.95
144_F 301_L 1.68 0.95
217_V 227_I 1.673 0.94
208_E 211_Q 1.65 0.94
116_S 136_K 1.647 0.94
512_L 516_Y 1.644 0.94
88_R 290_D 1.638 0.94
22_A 519_L 1.633 0.93
166_V 170_N 1.62 0.93
141_V 145_K 1.619 0.93
176_I 209_L 1.618 0.93
218_S 228_T 1.592 0.92
179_L 183_I 1.581 0.92
187_T 195_P 1.58 0.92
375_R 378_S 1.559 0.91
309_F 327_F 1.554 0.91
37_Q 197_N 1.554 0.91
327_F 433_I 1.551 0.91
15_A 18_A 1.536 0.90
501_S 505_Q 1.531 0.90
85_L 486_L 1.526 0.90
436_A 445_D 1.525 0.90
40_I 62_G 1.523 0.90
89_Y 483_T 1.521 0.90
244_L 258_V 1.521 0.90
434_A 448_N 1.516 0.90
72_T 504_Q 1.496 0.89
89_Y 487_K 1.494 0.89
313_H 427_I 1.485 0.88
82_S 86_T 1.484 0.88
215_V 227_I 1.482 0.88
212_I 279_S 1.481 0.88
535_T 539_I 1.473 0.88
144_F 302_A 1.471 0.88
112_D 136_K 1.461 0.87
316_G 448_N 1.454 0.87
308_A 311_T 1.45 0.87
531_Q 546_I 1.441 0.86
91_Q 286_F 1.435 0.86
75_L 500_L 1.429 0.86
44_A 56_N 1.422 0.85
99_L 484_A 1.42 0.85
162_I 287_R 1.417 0.85
175_Q 205_L 1.417 0.85
22_A 26_I 1.416 0.85
449_G 453_L 1.408 0.84
312_Q 448_N 1.406 0.84
529_N 532_V 1.406 0.84
541_D 545_N 1.405 0.84
165_S 280_L 1.404 0.84
176_I 179_L 1.4 0.84
166_V 244_L 1.393 0.83
113_F 140_L 1.386 0.83
526_Y 530_A 1.385 0.83
301_L 305_F 1.384 0.83
67_Y 72_T 1.382 0.83
4_L 536_A 1.38 0.83
367_D 370_Q 1.379 0.83
15_A 41_M 1.378 0.83
497_V 501_S 1.374 0.82
37_Q 200_D 1.374 0.82
166_V 258_V 1.373 0.82
173_A 235_L 1.37 0.82
364_I 373_V 1.357 0.81
534_Q 546_I 1.353 0.81
130_R 319_A 1.343 0.80
364_I 413_L 1.341 0.80
174_K 243_Q 1.34 0.80
106_L 147_T 1.318 0.79
165_S 279_S 1.311 0.78
82_S 497_V 1.307 0.78
493_Q 497_V 1.303 0.77
494_G 498_T 1.3 0.77
286_F 291_L 1.3 0.77
169_I 472_Y 1.296 0.77
17_A 20_N 1.295 0.77
285_T 289_Q 1.28 0.76
360_T 377_A 1.273 0.75
76_R 207_S 1.272 0.75
76_R 206_V 1.268 0.75
199_L 219_V 1.262 0.74
94_K 157_Q 1.257 0.74
318_D 323_A 1.256 0.74
498_T 502_N 1.255 0.74
82_S 490_S 1.242 0.73
173_A 213_V 1.238 0.72
33_G 204_Q 1.23 0.71
69_A 73_N 1.226 0.71
304_A 308_A 1.226 0.71
22_A 522_F 1.223 0.71
176_I 205_L 1.223 0.71
166_V 280_L 1.222 0.71
65_R 199_L 1.216 0.70
282_G 286_F 1.213 0.70
169_I 244_L 1.21 0.70
468_F 531_Q 1.202 0.69
313_H 452_L 1.199 0.69
72_T 207_S 1.197 0.68
72_T 544_I 1.197 0.68
312_Q 315_A 1.197 0.68
24_N 46_S 1.195 0.68
127_P 319_A 1.195 0.68
8_A 23_S 1.189 0.68
294_T 482_K 1.182 0.67
135_G 138_E 1.176 0.67
176_I 225_Y 1.174 0.66
123_N 126_D 1.173 0.66
38_T 64_Q 1.167 0.66
209_L 227_I 1.166 0.66
426_L 433_I 1.161 0.65
162_I 284_L 1.158 0.65
446_N 450_Q 1.156 0.65
4_L 23_S 1.155 0.64
525_Y 529_N 1.154 0.64
14_A 60_V 1.152 0.64
450_Q 454_D 1.144 0.63
15_A 525_Y 1.14 0.63
116_S 133_L 1.136 0.63
19_L 522_F 1.135 0.62
28_S 31_V 1.135 0.62
6_N 10_S 1.133 0.62
318_D 321_G 1.128 0.62
169_I 212_I 1.128 0.62
110_M 301_L 1.128 0.62
173_A 230_A 1.127 0.62
87_A 279_S 1.123 0.61
149_Q 153_D 1.115 0.60
430_E 434_A 1.11 0.60
158_V 286_F 1.109 0.60
418_D 421_V 1.109 0.60
78_A 493_Q 1.108 0.60
119_T 122_S 1.106 0.60
377_A 395_D 1.105 0.59
106_L 302_A 1.095 0.58
321_G 434_A 1.095 0.58
379_N 468_F 1.093 0.58
70_F 74_Q 1.092 0.58
79_Q 211_Q 1.085 0.57
255_R 292_D 1.084 0.57
39_T 60_V 1.084 0.57
175_Q 201_Q 1.08 0.57
168_Q 172_Y 1.075 0.56
11_G 76_R 1.073 0.56
159_N 288_S 1.07 0.56
505_Q 509_G 1.069 0.56
531_Q 545_N 1.067 0.55
4_L 519_L 1.064 0.55
511_N 514_E 1.062 0.55
536_A 539_I 1.061 0.55
71_I 507_I 1.061 0.55
531_Q 534_Q 1.059 0.55
35_T 65_R 1.058 0.54
36_R 515_E 1.058 0.54
327_F 453_L 1.057 0.54
47_T 56_N 1.051 0.54
476_V 479_I 1.051 0.54
11_G 15_A 1.049 0.53
26_I 210_N 1.048 0.53
33_G 55_G 1.041 0.53
36_R 514_E 1.04 0.53
163_G 167_D 1.037 0.52
363_K 410_S 1.034 0.52
144_F 512_L 1.031 0.52
37_Q 196_N 1.031 0.52
99_L 153_D 1.03 0.51
376_L 379_N 1.029 0.51
360_T 378_S 1.029 0.51
87_A 91_Q 1.029 0.51
40_I 64_Q 1.028 0.51
130_R 313_H 1.027 0.51
353_D 356_A 1.025 0.51
517_G 520_Q 1.024 0.51
172_Y 209_L 1.024 0.51
161_A 283_I 1.02 0.50
524_Q 527_L 1.019 0.50
297_T 538_A 1.017 0.50
35_T 200_D 1.015 0.50
121_V 449_G 1.013 0.50
342_K 406_A 1.011 0.49
130_R 316_G 1.005 0.49
109_Q 143_Q 1.003 0.49
313_H 325_E 1.002 0.48
151_L 295_R 1.001 0.48
527_L 539_I 1 0.48
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2d4yA20.83361000.274Contact Map0.777
3a69A10.64999.70.861Contact Map0.428
1oryB10.0987550.971Contact Map0.362
2l7aA10.201113.10.979Contact Map0.425
3r84A120.155412.80.979Contact Map0.716
1ykhB10.219412.60.979Contact Map0.187
3zccA20.202912.40.979Contact Map0.414
1zvaA10.1408120.98Contact Map0.362
2c5kT10.171811.70.98Contact Map0.096
1sj7A30.239510.30.98Contact Map0.327
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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