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OPENSEQ.org

NRFF - Formate-dependent nitrite reductase complex subunit NrfF
UniProt: P32711 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11949
Length: 127 (120)
Sequences: 619
Seq/Len: 5.16

NRFF
Paralog alert: 0.50 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
34_Q 77_E 3.029 1.00
90_D 97_P 2.876 1.00
45_P 59_V 2.389 1.00
98_L 107_A 2.354 1.00
82_G 86_E 2.192 1.00
31_Q 71_V 2.102 1.00
50_Q 56_N 2.074 1.00
90_D 99_T 2.073 1.00
70_M 75_K 1.998 1.00
78_V 82_G 1.979 1.00
46_Q 59_V 1.9 1.00
97_P 102_T 1.87 1.00
75_K 79_E 1.844 1.00
63_M 84_M 1.721 1.00
35_A 39_A 1.692 1.00
49_N 91_F 1.606 0.99
100_G 103_L 1.589 0.99
48_Q 91_F 1.56 0.99
123_V 126_K 1.537 0.99
66_Q 70_M 1.479 0.98
37_N 77_E 1.475 0.98
98_L 102_T 1.467 0.98
83_W 88_Y 1.388 0.97
39_A 52_L 1.349 0.97
8_L 11_L 1.337 0.97
22_D 26_F 1.335 0.96
66_Q 83_W 1.321 0.96
98_L 103_L 1.289 0.95
11_L 14_C 1.272 0.95
40_S 51_N 1.261 0.95
117_A 121_W 1.256 0.95
41_Q 95_N 1.244 0.94
23_T 27_A 1.241 0.94
67_V 92_V 1.232 0.94
60_A 88_Y 1.224 0.94
50_Q 54_E 1.219 0.93
66_Q 84_M 1.194 0.92
69_S 73_E 1.162 0.91
27_A 72_A 1.133 0.90
36_L 53_L 1.109 0.88
35_A 68_Y 1.108 0.88
8_L 14_C 1.083 0.86
70_M 73_E 1.083 0.86
35_A 53_L 1.082 0.86
37_N 41_Q 1.078 0.86
53_L 94_Y 1.071 0.86
21_V 26_F 1.07 0.86
118_L 121_W 1.065 0.85
67_V 80_I 1.06 0.85
32_Q 68_Y 1.059 0.85
83_W 93_R 1.053 0.84
26_F 68_Y 1.048 0.84
49_N 63_M 1.044 0.84
8_L 13_T 1.021 0.82
9_L 14_C 1.021 0.82
66_Q 69_S 1.012 0.81
20_V 23_T 1.005 0.80
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2hl7A10.66141000.306Contact Map0.424
2kw0A10.70871000.309Contact Map0.48
1w5cT20.732337.80.931Contact Map0.086
1odfA10.968527.90.935Contact Map0.121
1c2nA10.850424.10.937Contact Map0.135
2blfB10.5748180.941Contact Map0.25
3o0rC10.921317.20.941Contact Map0.1
2vxgA20.480311.70.945Contact Map0.38
4fasA30.503990.948Contact Map0.212
4aybP10.34657.50.95Contact Map0

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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