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OPENSEQ.org
HCHA - Molecular chaperone Hsp31 and glyoxalase 3
UniProt: P31658 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11755
Length: 283 (223)
Sequences: 901
Seq/Len: 4.04
HCHA
Paralog alert: 0.19 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
249_G 268_A 4.21 1.00
210_P 242_I 4.11 1.00
234_E 237_K 3.959 1.00
258_L 280_A 2.999 1.00
243_I 250_R 2.792 1.00
93_E 146_Y 2.56 1.00
276_E 279_A 2.485 1.00
211_D 233_E 2.374 1.00
199_N 238_M 2.326 1.00
205_S 241_N 2.219 1.00
151_V 167_V 2.216 1.00
50_K 91_E 2.215 1.00
205_S 243_I 2.208 1.00
176_K 197_G 2.192 1.00
234_E 238_M 2.093 1.00
232_G 236_K 2.054 1.00
253_K 280_A 2.037 1.00
142_A 145_E 2.024 1.00
174_A 179_R 2.005 1.00
204_Y 255_R 1.936 1.00
182_I 258_L 1.925 1.00
84_H 275_Q 1.922 1.00
51_I 90_F 1.909 1.00
132_K 135_D 1.866 1.00
109_M 121_E 1.834 1.00
175_I 256_K 1.827 1.00
180_F 277_M 1.807 1.00
176_K 196_H 1.795 1.00
251_V 276_E 1.76 0.99
95_A 133_L 1.696 0.99
231_F 235_L 1.678 0.99
56_A 156_G 1.642 0.99
76_I 266_A 1.595 0.99
163_E 193_A 1.594 0.99
101_M 128_R 1.591 0.99
134_A 166_D 1.57 0.98
167_V 190_A 1.544 0.98
51_I 85_L 1.498 0.98
83_Y 87_A 1.48 0.97
178_D 256_K 1.478 0.97
215_K 224_P 1.462 0.97
253_K 258_L 1.446 0.97
131_K 135_D 1.445 0.97
275_Q 279_A 1.43 0.97
78_T 94_V 1.423 0.96
175_I 204_Y 1.388 0.96
143_D 173_W 1.386 0.96
54_I 167_V 1.382 0.96
183_S 191_F 1.373 0.95
94_V 126_L 1.371 0.95
51_I 148_A 1.368 0.95
193_A 238_M 1.357 0.95
151_V 190_A 1.343 0.95
85_L 277_M 1.324 0.94
171_L 194_L 1.323 0.94
205_S 252_H 1.321 0.94
152_P 161_L 1.319 0.94
169_A 173_W 1.299 0.93
253_K 276_E 1.291 0.93
248_T 264_F 1.286 0.93
268_A 276_E 1.281 0.93
53_V 92_F 1.273 0.92
231_F 234_E 1.256 0.92
157_A 161_L 1.256 0.92
171_L 181_V 1.252 0.91
143_D 174_A 1.239 0.91
208_A 242_I 1.234 0.91
192_L 234_E 1.23 0.91
182_I 277_M 1.221 0.90
86_H 92_F 1.212 0.90
85_L 274_A 1.196 0.89
131_K 136_V 1.192 0.89
53_V 85_L 1.164 0.87
85_L 92_F 1.157 0.87
148_A 277_M 1.155 0.86
266_A 274_A 1.144 0.86
84_H 278_L 1.138 0.85
60_Y 70_S 1.135 0.85
168_A 172_Q 1.129 0.85
73_N 102_T 1.126 0.84
172_Q 176_K 1.118 0.84
258_L 269_L 1.117 0.84
189_A 229_W 1.111 0.83
82_L 94_V 1.106 0.83
81_P 150_F 1.1 0.83
213_A 261_D 1.094 0.82
144_S 173_W 1.085 0.81
133_L 167_V 1.081 0.81
207_C 242_I 1.057 0.79
79_L 115_K 1.055 0.79
181_V 256_K 1.042 0.78
136_V 146_Y 1.039 0.77
221_G 224_P 1.036 0.77
174_A 181_V 1.035 0.77
115_K 267_N 1.035 0.77
220_I 223_M 1.025 0.76
54_I 95_A 1.024 0.76
162_P 193_A 1.016 0.75
97_I 157_A 1.013 0.75
175_I 200_P 1.009 0.74
133_L 171_L 1.007 0.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1n57A10.98591000.46Contact Map0.62
4i2nA60.98231000.471Contact Map0.651
3n7tA10.80571000.577Contact Map0.676
4lruA10.7881000.577Contact Map0.781
1rw7A10.78451000.578Contact Map0.72
3kklA20.78451000.582Contact Map0.82
1u9cA10.77391000.594Contact Map0.856
3l3bA20.76681000.661Contact Map0.658
1vhqA20.70671000.677Contact Map0.674
3uk7A30.69261000.697Contact Map0.733
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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