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OPENSEQ.org

PANB - 3-methyl-2-oxobutanoate hydroxymethyltransferase
UniProt: P31057 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11675
Length: 264 (263)
Sequences: 1511
Seq/Len: 5.75

PANB
Paralog alert: 0.04 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
18_R 254_G 3.79 1.00
235_A 238_G 3.16 1.00
88_M 101_A 3.062 1.00
189_K 193_E 3.056 1.00
123_V 170_L 2.979 1.00
21_T 212_I 2.89 1.00
233_F 250_E 2.822 1.00
48_G 60_V 2.711 1.00
69_T 83_A 2.566 1.00
62_V 99_N 2.554 1.00
189_K 207_V 2.466 1.00
171_E 195_L 2.453 1.00
192_T 199_V 2.419 1.00
231_K 261_H 2.398 1.00
62_V 89_A 2.36 1.00
171_E 196_A 2.359 1.00
13_K 209_D 2.359 1.00
231_K 250_E 2.332 1.00
52_Q 67_Y 2.329 1.00
17_K 252_E 2.187 1.00
117_E 165_S 2.124 1.00
95_Q 98_E 2.087 1.00
85_L 99_N 2.077 1.00
168_L 194_A 2.075 1.00
124_Q 128_E 2.058 1.00
20_A 248_M 1.994 1.00
239_D 242_A 1.952 1.00
134_C 177_L 1.929 1.00
12_Y 19_F 1.912 1.00
134_C 176_Q 1.879 1.00
161_D 165_S 1.836 1.00
73_R 81_L 1.832 1.00
33_F 41_M 1.83 1.00
135_G 170_L 1.815 1.00
249_A 253_S 1.796 1.00
56_S 59_P 1.775 1.00
38_L 248_M 1.768 1.00
11_K 15_E 1.749 1.00
234_L 239_D 1.741 1.00
248_M 252_E 1.735 1.00
190_R 194_A 1.698 1.00
33_F 244_V 1.681 1.00
162_Q 166_D 1.675 1.00
64_D 70_A 1.661 1.00
40_V 212_I 1.646 1.00
258_G 261_H 1.64 1.00
34_A 79_C 1.636 1.00
170_L 175_A 1.622 0.99
226_I 232_N 1.607 0.99
62_V 103_V 1.594 0.99
175_A 197_I 1.587 0.99
168_L 171_E 1.563 0.99
69_T 108_A 1.525 0.99
9_L 40_V 1.514 0.99
66_A 103_V 1.514 0.99
178_L 199_V 1.513 0.99
69_T 106_A 1.501 0.99
153_Q 159_A 1.466 0.99
90_Y 113_I 1.461 0.99
250_E 255_V 1.454 0.99
238_G 242_A 1.449 0.99
171_E 197_I 1.434 0.98
12_Y 17_K 1.433 0.98
63_A 102_T 1.427 0.98
83_A 106_A 1.424 0.98
135_G 175_A 1.422 0.98
135_G 179_V 1.419 0.98
168_L 195_L 1.418 0.98
140_T 147_F 1.411 0.98
103_V 106_A 1.407 0.98
156_G 159_A 1.405 0.98
125_M 129_R 1.388 0.98
242_A 246_Q 1.367 0.98
52_Q 71_A 1.36 0.98
83_A 103_V 1.347 0.97
96_A 122_T 1.342 0.97
76_A 81_L 1.338 0.97
178_L 195_L 1.336 0.97
93_P 122_T 1.328 0.97
110_M 176_Q 1.313 0.97
137_L 180_L 1.308 0.97
185_V 262_S 1.308 0.97
82_L 179_V 1.3 0.97
54_H 74_R 1.288 0.96
109_N 145_N 1.287 0.96
66_A 106_A 1.267 0.96
29_F 215_M 1.26 0.96
219_F 240_I 1.251 0.95
206_N 262_S 1.251 0.95
22_I 218_A 1.251 0.95
41_M 76_A 1.243 0.95
178_L 197_I 1.231 0.95
179_V 197_I 1.228 0.95
19_F 210_G 1.216 0.94
33_F 215_M 1.189 0.94
85_L 100_A 1.181 0.93
137_L 163_L 1.177 0.93
164_L 190_R 1.174 0.93
118_W 162_Q 1.164 0.93
21_T 40_V 1.158 0.92
54_H 59_P 1.149 0.92
232_N 235_A 1.148 0.92
97_F 126_L 1.146 0.92
34_A 78_N 1.143 0.92
82_L 110_M 1.139 0.91
192_T 208_T 1.139 0.91
97_F 125_M 1.132 0.91
51_V 216_H 1.12 0.91
20_A 252_E 1.116 0.90
185_V 207_V 1.115 0.90
199_V 208_T 1.106 0.90
54_H 64_D 1.106 0.90
48_G 64_D 1.094 0.89
35_D 241_R 1.075 0.88
93_P 125_M 1.071 0.88
47_L 68_H 1.07 0.88
95_Q 99_N 1.067 0.87
34_A 77_P 1.064 0.87
222_T 225_H 1.057 0.87
47_L 60_V 1.047 0.86
121_E 124_Q 1.043 0.86
139_L 182_C 1.027 0.85
245_R 249_A 1.024 0.84
66_A 102_T 1.016 0.84
188_A 208_T 1.012 0.83
120_V 166_D 1.008 0.83
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1o66A50.99621000.197Contact Map0.811
1m3uA1011000.198Contact Map0.783
1oy0A50.99241000.205Contact Map0.653
3vavA100.99621000.217Contact Map0.836
4lsbA20.90531000.522Contact Map0.477
4mg4A80.88641000.59Contact Map0.558
3lyeA10.92421000.609Contact Map0.463
2qiwA20.88261000.609Contact Map0.607
3eooA160.91671000.615Contact Map0.528
1xg4A40.91671000.616Contact Map0.519

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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