May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org

RFAL - O-antigen ligase
UniProt: P27243 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11424
Length: 419 (395)
Sequences: 508
Seq/Len: 1.29

RFAL
Paralog alert: 0.12 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
299_F 345_G 2.373 0.99
215_R 265_R 2.003 0.97
283_T 286_G 1.933 0.96
353_I 357_L 1.724 0.92
295_G 342_I 1.724 0.92
292_Y 342_I 1.645 0.90
345_G 353_I 1.636 0.89
296_L 300_I 1.587 0.87
28_L 32_S 1.575 0.87
299_F 303_P 1.548 0.86
336_H 390_V 1.529 0.85
337_L 343_E 1.529 0.85
269_A 272_D 1.514 0.84
166_V 304_F 1.506 0.83
336_H 389_D 1.502 0.83
310_E 347_L 1.48 0.82
301_K 319_L 1.478 0.82
35_I 39_D 1.474 0.81
388_S 406_V 1.466 0.81
214_T 301_K 1.457 0.80
194_H 223_I 1.453 0.80
199_L 243_S 1.453 0.80
336_H 394_A 1.424 0.78
344_A 356_T 1.421 0.78
223_I 384_G 1.397 0.76
172_A 210_A 1.39 0.76
343_E 347_L 1.389 0.76
287_A 291_M 1.386 0.76
161_R 214_T 1.371 0.75
265_R 268_E 1.371 0.75
54_C 57_S 1.37 0.74
216_A 337_L 1.37 0.74
117_S 173_A 1.365 0.74
363_L 401_I 1.363 0.74
29_C 35_I 1.354 0.73
26_Y 30_F 1.336 0.72
39_D 42_F 1.332 0.71
119_I 143_S 1.328 0.71
308_S 312_R 1.327 0.71
354_F 365_Y 1.325 0.71
308_S 337_L 1.323 0.71
339_N 394_A 1.316 0.70
148_G 251_L 1.302 0.69
302_S 307_R 1.301 0.69
229_L 253_S 1.3 0.69
144_F 148_G 1.289 0.68
250_I 254_I 1.286 0.68
201_N 247_L 1.286 0.68
295_G 337_L 1.282 0.67
30_F 33_M 1.272 0.66
169_A 214_T 1.265 0.66
197_L 304_F 1.257 0.65
204_A 246_L 1.257 0.65
348_K 352_G 1.253 0.65
147_A 205_V 1.253 0.65
244_I 248_I 1.25 0.64
31_F 184_G 1.25 0.64
312_R 316_M 1.248 0.64
250_I 274_N 1.245 0.64
218_L 337_L 1.242 0.64
230_I 251_L 1.24 0.63
214_T 288_R 1.236 0.63
308_S 347_L 1.234 0.63
354_F 358_F 1.233 0.63
176_T 180_G 1.233 0.63
88_W 111_K 1.231 0.63
169_A 213_Q 1.224 0.62
46_I 57_S 1.217 0.61
162_I 181_I 1.215 0.61
106_Y 340_E 1.213 0.61
283_T 287_A 1.213 0.61
174_Y 389_D 1.213 0.61
350_L 354_F 1.211 0.61
83_L 144_F 1.207 0.60
153_I 336_H 1.203 0.60
168_T 310_E 1.201 0.60
148_G 387_L 1.197 0.59
161_R 213_Q 1.191 0.59
170_T 308_S 1.191 0.59
161_R 169_A 1.188 0.59
304_F 351_M 1.186 0.58
77_S 359_L 1.186 0.58
50_T 59_I 1.182 0.58
203_C 369_K 1.182 0.58
340_E 386_G 1.18 0.58
252_A 345_G 1.176 0.57
216_A 389_D 1.173 0.57
286_G 402_I 1.172 0.57
389_D 394_A 1.171 0.57
64_Q 357_L 1.171 0.57
152_Y 291_M 1.169 0.57
200_L 204_A 1.162 0.56
49_I 53_V 1.16 0.56
116_G 317_N 1.157 0.56
339_N 342_I 1.151 0.55
201_N 255_V 1.148 0.55
180_G 224_I 1.145 0.54
307_R 389_D 1.143 0.54
251_L 382_I 1.142 0.54
197_L 200_L 1.137 0.54
292_Y 388_S 1.136 0.53
22_E 25_T 1.135 0.53
262_I 409_L 1.134 0.53
56_L 244_I 1.131 0.53
23_I 27_I 1.129 0.53
226_V 354_F 1.129 0.53
170_T 216_A 1.128 0.53
218_L 307_R 1.125 0.52
111_K 116_G 1.124 0.52
340_E 402_I 1.124 0.52
80_L 354_F 1.12 0.52
375_L 386_G 1.12 0.52
53_V 57_S 1.119 0.52
351_M 354_F 1.113 0.51
359_L 368_Y 1.112 0.51
226_V 311_S 1.111 0.51
312_R 343_E 1.109 0.51
111_K 311_S 1.108 0.51
291_M 309_A 1.108 0.51
294_I 337_L 1.108 0.51
76_L 403_S 1.106 0.50
174_Y 307_R 1.105 0.50
198_F 251_L 1.104 0.50
297_N 300_I 1.103 0.50
63_R 254_I 1.1 0.50
229_L 350_L 1.1 0.50
281_S 329_A 1.1 0.50
307_R 346_S 1.093 0.49
170_T 214_T 1.092 0.49
133_E 234_N 1.09 0.49
37_F 41_T 1.089 0.49
31_F 35_I 1.089 0.49
365_Y 369_K 1.087 0.49
170_T 312_R 1.086 0.48
168_T 291_M 1.085 0.48
340_E 360_Y 1.084 0.48
289_L 400_I 1.084 0.48
22_E 26_Y 1.083 0.48
129_K 194_H 1.083 0.48
291_M 310_E 1.082 0.48
74_L 354_F 1.079 0.48
296_L 380_L 1.079 0.48
40_N 44_I 1.072 0.47
249_A 350_L 1.07 0.47
106_Y 360_Y 1.07 0.47
340_E 397_I 1.067 0.47
88_W 218_L 1.065 0.46
229_L 247_L 1.06 0.46
192_K 195_P 1.059 0.46
351_M 384_G 1.056 0.46
280_N 286_G 1.052 0.45
220_L 260_K 1.051 0.45
283_T 291_M 1.049 0.45
295_G 299_F 1.044 0.44
192_K 229_L 1.041 0.44
371_R 380_L 1.04 0.44
212_T 393_W 1.04 0.44
148_G 204_A 1.04 0.44
221_F 369_K 1.04 0.44
67_Y 341_I 1.039 0.44
217_T 410_V 1.038 0.44
46_I 49_I 1.038 0.44
390_V 394_A 1.037 0.44
112_I 404_A 1.036 0.44
214_T 385_I 1.036 0.44
149_Y 162_I 1.036 0.44
168_T 346_S 1.033 0.43
169_A 282_V 1.031 0.43
247_L 368_Y 1.029 0.43
213_Q 282_V 1.029 0.43
130_S 192_K 1.028 0.43
56_L 408_L 1.028 0.43
173_A 197_L 1.027 0.43
202_S 248_I 1.027 0.43
84_L 353_I 1.027 0.43
112_I 181_I 1.023 0.42
312_R 337_L 1.021 0.42
153_I 157_H 1.021 0.42
377_I 384_G 1.021 0.42
138_T 193_N 1.02 0.42
49_I 63_R 1.02 0.42
341_I 383_V 1.02 0.42
30_F 39_D 1.02 0.42
212_T 389_D 1.019 0.42
390_V 397_I 1.017 0.42
265_R 284_S 1.016 0.42
216_A 394_A 1.015 0.42
91_A 135_V 1.014 0.41
365_Y 384_G 1.013 0.41
192_K 354_F 1.013 0.41
343_E 346_S 1.013 0.41
216_A 307_R 1.013 0.41
257_I 267_N 1.012 0.41
183_S 225_C 1.012 0.41
52_I 354_F 1.011 0.41
151_M 380_L 1.01 0.41
166_V 397_I 1.01 0.41
135_V 400_I 1.009 0.41
32_S 37_F 1.008 0.41
229_L 297_N 1.007 0.41
73_I 358_F 1.007 0.41
88_W 92_F 1.005 0.41
63_R 66_N 1.005 0.41
214_T 347_L 1.005 0.41
200_L 203_C 1.005 0.41
262_I 364_F 1.004 0.40
197_L 219_L 1.003 0.40
149_Y 178_L 1.003 0.40
210_A 374_G 1.002 0.40
340_E 367_A 1.002 0.40
245_V 249_A 1.001 0.40
161_R 202_S 1.001 0.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results:
PDB Chains Cov P(%) HHΔ   Acc
2mc7A10.071640.972Contact Map1
1zkeA60.16712.60.974Contact Map0.591
1iijA10.08352.30.975Contact Map0.417
1dx7A10.11222.20.975Contact Map0.244
3hxiC10.05012.20.975Contact Map0.419
2k1kA20.08831.90.976Contact Map0.249
1lghB40.10741.60.977Contact Map0.348
2k1aA10.09791.50.977Contact Map0.405
4jc9S140.11461.50.977Contact Map0.468
2l2tA20.1051.20.979Contact Map0.396

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0175 seconds.