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OPENSEQ.org
MAO1 - NAD-dependent malic enzyme
UniProt: P26616 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10948
Length: 565 (556)
Sequences: 830
Seq/Len: 1.49
MAO1
Paralog alert: 0.15 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
398_E 402_R 6.633 1.00
202_Q 206_N 6.251 1.00
152_V 230_F 3.387 1.00
485_E 533_Q 3.358 1.00
92_V 103_I 3.352 1.00
148_K 189_Y 3.157 1.00
64_Y 99_M 3.067 1.00
261_R 484_D 3.003 1.00
401_I 433_W 2.988 1.00
405_H 434_T 2.91 1.00
312_E 355_L 2.89 1.00
224_Y 255_P 2.87 1.00
299_V 377_V 2.829 1.00
202_Q 216_P 2.773 1.00
195_L 227_V 2.743 1.00
136_D 236_Q 2.717 1.00
428_Q 432_A 2.629 1.00
483_T 535_V 2.623 1.00
402_R 435_E 2.581 1.00
133_H 229_E 2.526 1.00
221_D 225_E 2.452 1.00
90_R 94_N 2.383 1.00
403_E 407_H 2.372 1.00
500_L 504_G 2.355 1.00
435_E 452_K 2.334 0.99
245_F 266_S 2.275 0.99
401_I 413_V 2.228 0.99
110_A 114_R 2.221 0.99
231_I 264_I 2.216 0.99
403_E 406_K 2.204 0.99
425_A 430_I 2.179 0.99
230_F 234_V 2.133 0.99
228_D 263_E 2.108 0.99
529_M 532_Q 2.103 0.99
405_H 437_N 2.102 0.99
379_R 403_E 2.073 0.99
375_L 399_E 2.054 0.98
60_A 91_L 2.051 0.98
426_T 429_D 2.048 0.98
343_D 358_K 2.029 0.98
200_N 219_T 1.994 0.98
337_R 352_Q 1.973 0.98
469_G 473_G 1.942 0.97
404_M 413_V 1.919 0.97
323_L 331_K 1.919 0.97
135_M 226_F 1.897 0.97
28_K 36_E 1.85 0.96
375_L 400_I 1.804 0.96
409_P 437_N 1.784 0.95
67_F 72_D 1.773 0.95
275_A 313_M 1.769 0.95
61_W 95_H 1.765 0.95
524_F 548_I 1.758 0.95
431_I 451_W 1.735 0.94
394_G 429_D 1.727 0.94
483_T 533_Q 1.723 0.94
127_I 135_M 1.721 0.94
529_M 533_Q 1.714 0.94
486_M 530_A 1.704 0.93
140_Q 237_R 1.701 0.93
105_T 444_S 1.69 0.93
152_V 234_V 1.689 0.93
156_E 252_N 1.688 0.93
97_D 513_K 1.685 0.93
282_L 314_I 1.682 0.93
228_D 259_R 1.676 0.93
193_V 226_F 1.671 0.93
38_R 43_L 1.665 0.92
310_I 389_V 1.663 0.92
520_R 549_D 1.64 0.92
298_I 318_T 1.634 0.91
252_N 255_P 1.622 0.91
413_V 430_I 1.622 0.91
292_Q 295_E 1.621 0.91
65_Q 98_E 1.593 0.90
95_H 98_E 1.582 0.90
281_T 509_L 1.564 0.89
221_D 224_Y 1.546 0.88
17_G 20_L 1.544 0.88
474_V 482_I 1.532 0.88
285_A 412_I 1.531 0.88
70_E 73_K 1.515 0.87
450_V 455_I 1.514 0.87
156_E 249_A 1.502 0.86
53_I 86_T 1.5 0.86
51_E 59_R 1.496 0.86
157_R 161_L 1.494 0.86
85_E 123_R 1.493 0.86
341_L 352_Q 1.481 0.85
324_S 327_A 1.467 0.84
473_G 530_A 1.451 0.83
301_L 392_Q 1.445 0.83
129_Y 218_I 1.445 0.83
243_L 264_I 1.444 0.83
205_L 216_P 1.429 0.82
400_I 403_E 1.427 0.82
52_T 55_E 1.418 0.81
301_L 395_L 1.412 0.81
473_G 526_V 1.405 0.81
398_E 435_E 1.405 0.81
335_V 374_L 1.404 0.81
466_I 470_I 1.402 0.80
224_Y 252_N 1.399 0.80
373_S 376_D 1.396 0.80
479_A 486_M 1.389 0.80
368_D 376_D 1.382 0.79
320_R 492_E 1.379 0.79
402_R 434_T 1.371 0.78
391_G 395_L 1.361 0.78
105_T 109_G 1.353 0.77
282_L 298_I 1.351 0.77
425_A 442_T 1.349 0.77
338_F 352_Q 1.348 0.77
32_F 36_E 1.344 0.76
543_A 546_Q 1.343 0.76
109_G 467_F 1.335 0.76
130_Q 213_W 1.335 0.76
240_D 478_G 1.334 0.76
524_F 545_Q 1.332 0.75
483_T 486_M 1.329 0.75
483_T 529_M 1.32 0.75
401_I 430_I 1.308 0.74
397_T 400_I 1.303 0.73
378_V 400_I 1.3 0.73
228_D 232_Q 1.297 0.73
231_I 245_F 1.297 0.73
425_A 440_V 1.295 0.73
296_K 384_D 1.294 0.72
195_L 226_F 1.292 0.72
69_T 72_D 1.289 0.72
193_V 230_F 1.272 0.71
57_A 90_R 1.268 0.70
379_R 399_E 1.267 0.70
112_C 157_R 1.264 0.70
53_I 90_R 1.263 0.70
227_V 256_L 1.259 0.69
382_K 407_H 1.259 0.69
299_V 335_V 1.258 0.69
348_L 356_V 1.247 0.68
277_V 515_I 1.246 0.68
57_A 94_N 1.244 0.68
228_D 260_Y 1.244 0.68
150_I 234_V 1.242 0.68
156_E 200_N 1.241 0.68
514_D 517_K 1.239 0.68
135_M 139_L 1.237 0.68
297_K 381_V 1.221 0.66
398_E 434_T 1.219 0.66
341_L 356_V 1.211 0.65
105_T 467_F 1.207 0.65
254_M 352_Q 1.204 0.64
398_E 433_W 1.199 0.64
476_A 527_G 1.198 0.64
294_S 321_E 1.188 0.63
439_L 508_V 1.188 0.63
286_S 292_Q 1.184 0.63
127_I 138_I 1.175 0.62
466_I 494_L 1.169 0.61
461_C 509_L 1.168 0.61
185_I 472_L 1.168 0.61
357_Q 381_V 1.161 0.60
46_L 50_V 1.16 0.60
16_A 40_F 1.155 0.60
470_I 487_L 1.148 0.59
516_Q 552_F 1.146 0.59
241_V 256_L 1.14 0.58
161_L 170_G 1.135 0.58
27_N 32_F 1.123 0.57
129_Y 226_F 1.122 0.56
130_Q 215_N 1.12 0.56
431_I 449_V 1.118 0.56
85_E 120_R 1.118 0.56
442_T 458_I 1.115 0.56
265_C 474_V 1.113 0.55
201_N 204_L 1.111 0.55
129_Y 213_W 1.109 0.55
430_I 442_T 1.109 0.55
81_Q 123_R 1.106 0.55
337_R 341_L 1.104 0.55
477_S 527_G 1.102 0.54
281_T 459_A 1.099 0.54
79_N 83_T 1.091 0.53
545_Q 549_D 1.09 0.53
427_P 440_V 1.087 0.53
132_R 229_E 1.086 0.53
215_N 218_I 1.084 0.53
174_G 181_A 1.083 0.52
473_G 479_A 1.082 0.52
112_C 161_L 1.081 0.52
20_L 40_F 1.079 0.52
112_C 158_I 1.078 0.52
132_R 225_E 1.076 0.52
115_F 378_V 1.076 0.52
61_W 98_E 1.076 0.52
546_Q 549_D 1.075 0.52
30_S 211_M 1.075 0.52
528_K 541_A 1.073 0.51
277_V 494_L 1.073 0.51
332_V 357_Q 1.07 0.51
315_I 329_R 1.07 0.51
235_K 264_I 1.067 0.51
487_L 491_S 1.064 0.50
385_I 390_S 1.061 0.50
115_F 119_Y 1.06 0.50
431_I 456_Y 1.059 0.50
183_G 249_A 1.051 0.49
261_R 397_T 1.048 0.49
49_V 214_R 1.048 0.49
402_R 437_N 1.046 0.49
307_G 483_T 1.042 0.48
157_R 170_G 1.042 0.48
61_W 99_M 1.041 0.48
139_L 237_R 1.04 0.48
93_N 516_Q 1.039 0.48
282_L 385_I 1.039 0.48
117_E 207_D 1.039 0.48
521_A 545_Q 1.038 0.48
42_L 46_L 1.038 0.48
35_E 39_N 1.035 0.47
520_R 524_F 1.035 0.47
81_Q 85_E 1.034 0.47
520_R 545_Q 1.034 0.47
136_D 237_R 1.034 0.47
37_R 46_L 1.031 0.47
327_A 428_Q 1.03 0.47
82_D 123_R 1.03 0.47
125_V 142_V 1.029 0.47
430_I 440_V 1.028 0.47
112_C 275_A 1.025 0.46
147_I 237_R 1.025 0.46
250_Q 305_S 1.021 0.46
431_I 458_I 1.021 0.46
56_Q 87_L 1.021 0.46
61_W 65_Q 1.019 0.46
112_C 155_G 1.017 0.46
345_M 352_Q 1.011 0.45
401_I 434_T 1.011 0.45
115_F 173_I 1.01 0.45
112_C 170_G 1.009 0.45
520_R 552_F 1.005 0.44
299_V 381_V 1.004 0.44
546_Q 550_D 1.002 0.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1o0sA20.98411000.128Contact Map0.706
1pj3A40.96641000.13Contact Map0.744
1gq2A160.95931000.131Contact Map0.739
3nv9A20.79651000.527Contact Map0.481
1vl6A40.66551000.6Contact Map0.528
2a9fA20.69561000.656Contact Map0.364
2dvmA40.7311000.665Contact Map0.551
3aoeE40.665598.90.955Contact Map0.408
3aogA120.727498.80.955Contact Map0.301
1v8bA40.748798.80.956Contact Map0.501
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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