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OPENSEQ.org
PTRB - Protease 2
UniProt: P24555 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11004
Length: 686 (675)
Sequences: 2509
Seq/Len: 3.72
PTRB
Paralog alert: 0.28 [within 20: 0.03] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
424_R 510_D 3.959 1.00
422_V 428_E 3.85 1.00
542_I 552_V 3.486 1.00
513_D 548_L 3.409 1.00
159_N 162_T 3.314 1.00
417_E 435_Y 3.106 1.00
586_P 590_E 3.089 1.00
410_Y 413_N 3.03 1.00
13_T 18_T 2.976 1.00
7_R 634_E 2.958 1.00
513_D 545_R 2.945 1.00
281_R 297_R 2.872 1.00
522_S 525_L 2.861 1.00
531_G 555_Q 2.778 1.00
538_M 552_V 2.74 1.00
431_V 511_A 2.71 1.00
6_A 35_E 2.666 1.00
231_T 238_V 2.557 1.00
512_C 549_F 2.55 1.00
139_T 161_E 2.538 1.00
392_D 395_T 2.485 1.00
235_H 253_D 2.367 1.00
585_D 588_Y 2.343 1.00
159_N 164_N 2.311 1.00
387_T 400_V 2.309 1.00
433_L 478_A 2.286 1.00
17_D 593_K 2.28 1.00
514_A 518_L 2.238 1.00
553_I 672_L 2.231 1.00
449_V 512_C 2.226 1.00
421_I 431_V 2.217 1.00
611_V 629_V 2.186 1.00
59_D 63_K 2.172 1.00
377_Y 398_R 2.162 1.00
62_L 66_I 2.157 1.00
502_K 544_Q 2.149 1.00
142_A 179_W 2.147 1.00
513_D 517_K 2.145 1.00
204_H 213_D 2.125 1.00
447_L 526_C 2.09 1.00
420_W 428_E 2.084 1.00
445_N 476_V 2.074 1.00
631_K 635_L 2.067 1.00
157_F 186_F 2.058 1.00
527_Y 672_L 2.048 1.00
417_E 441_R 2.035 1.00
498_F 588_Y 2.032 1.00
158_R 165_W 2.028 1.00
421_I 511_A 2.027 1.00
202_W 215_L 2.017 1.00
235_H 255_E 2.013 1.00
449_V 549_F 2.009 1.00
142_A 177_F 1.987 1.00
506_N 544_Q 1.974 1.00
528_A 541_A 1.968 1.00
38_D 41_Q 1.951 1.00
253_D 256_M 1.934 1.00
439_H 445_N 1.931 1.00
11_A 18_T 1.93 1.00
38_D 42_Q 1.929 1.00
137_D 183_S 1.906 1.00
587_Q 590_E 1.866 1.00
636_K 642_L 1.851 1.00
421_I 480_V 1.843 1.00
527_Y 551_G 1.825 0.99
295_L 318_L 1.817 0.99
550_H 608_H 1.812 0.99
542_I 609_L 1.797 0.99
509_L 549_F 1.791 0.99
297_R 309_L 1.785 0.99
424_R 506_N 1.784 0.99
447_L 521_G 1.776 0.99
180_A 185_I 1.754 0.99
521_G 526_C 1.746 0.99
528_A 552_V 1.741 0.99
381_S 384_T 1.73 0.99
140_I 157_F 1.721 0.99
37_L 41_Q 1.717 0.99
512_C 526_C 1.71 0.99
53_S 674_A 1.701 0.99
543_N 628_W 1.697 0.99
451_G 508_Y 1.669 0.99
86_R 99_Q 1.665 0.99
643_L 667_M 1.661 0.99
54_Q 58_Q 1.661 0.99
34_P 38_D 1.655 0.99
440_F 443_G 1.625 0.99
531_G 653_G 1.62 0.98
553_I 668_E 1.615 0.98
294_G 308_E 1.612 0.98
389_F 400_V 1.602 0.98
444_H 522_S 1.6 0.98
516_L 521_G 1.6 0.98
547_E 605_A 1.599 0.98
458_S 487_E 1.593 0.98
473_R 673_V 1.585 0.98
339_S 352_G 1.581 0.98
601_V 628_W 1.579 0.98
124_S 147_F 1.579 0.98
434_V 471_L 1.578 0.98
290_G 294_G 1.568 0.98
449_V 508_Y 1.55 0.98
516_L 526_C 1.544 0.98
415_R 438_K 1.542 0.98
527_Y 676_A 1.529 0.98
448_L 672_L 1.526 0.98
112_E 163_G 1.523 0.97
608_H 641_L 1.501 0.97
180_A 184_W 1.493 0.97
647_D 663_E 1.492 0.97
502_K 594_S 1.485 0.97
645_C 667_M 1.481 0.97
327_W 342_Q 1.479 0.97
9_P 22_N 1.476 0.97
431_V 478_A 1.472 0.97
600_N 603_A 1.471 0.97
373_A 394_D 1.469 0.97
448_L 529_M 1.466 0.97
419_L 431_V 1.464 0.97
151_R 619_Q 1.457 0.96
48_H 52_A 1.452 0.96
611_V 628_W 1.443 0.96
490_Q 494_E 1.441 0.96
589_Y 593_K 1.44 0.96
495_D 500_K 1.424 0.96
17_D 589_Y 1.423 0.96
475_F 673_V 1.423 0.96
142_A 155_I 1.422 0.96
238_V 251_L 1.419 0.96
551_G 675_L 1.414 0.96
508_Y 528_A 1.414 0.96
499_L 588_Y 1.41 0.96
491_Q 495_D 1.407 0.95
136_P 183_S 1.393 0.95
373_A 392_D 1.391 0.95
502_K 506_N 1.389 0.95
660_K 663_E 1.385 0.95
509_L 545_R 1.383 0.95
420_W 488_L 1.372 0.95
450_Y 459_I 1.369 0.94
139_T 159_N 1.365 0.94
447_L 476_V 1.362 0.94
28_D 33_Q 1.361 0.94
499_L 591_Y 1.355 0.94
641_L 675_L 1.355 0.94
478_A 515_L 1.354 0.94
200_Q 218_E 1.353 0.94
99_Q 113_T 1.351 0.94
86_R 111_W 1.35 0.94
135_T 179_W 1.35 0.94
286_S 296_Y 1.347 0.94
90_E 93_C 1.346 0.94
419_L 514_A 1.334 0.93
494_E 497_K 1.332 0.93
187_Y 237_V 1.331 0.93
61_I 65_I 1.33 0.93
382_M 432_S 1.326 0.93
499_L 502_K 1.32 0.93
622_Y 646_T 1.32 0.93
7_R 22_N 1.32 0.93
185_I 203_R 1.32 0.93
188_Y 213_D 1.319 0.93
529_M 555_Q 1.309 0.93
556_V 653_G 1.304 0.92
482_V 504_T 1.303 0.92
470_L 475_F 1.303 0.92
622_Y 626_A 1.296 0.92
425_D 483_R 1.296 0.92
516_L 548_L 1.292 0.92
374_R 390_E 1.287 0.92
130_G 144_A 1.285 0.92
334_Q 339_S 1.283 0.92
421_I 514_A 1.281 0.92
509_L 513_D 1.281 0.92
540_V 544_Q 1.278 0.91
476_V 520_Y 1.276 0.91
524_S 550_H 1.273 0.91
663_E 667_M 1.268 0.91
545_R 548_L 1.267 0.91
43_E 633_R 1.262 0.91
342_Q 351_I 1.257 0.90
136_P 179_W 1.254 0.90
506_N 510_D 1.249 0.90
295_L 321_F 1.244 0.90
635_L 640_H 1.241 0.90
374_R 392_D 1.239 0.90
467_R 669_Y 1.235 0.89
344_N 348_R 1.233 0.89
391_L 396_G 1.233 0.89
344_N 347_T 1.229 0.89
563_T 627_K 1.229 0.89
422_V 429_V 1.226 0.89
591_Y 594_S 1.226 0.89
41_Q 45_S 1.225 0.89
135_T 142_A 1.223 0.89
432_S 481_H 1.217 0.89
560_D 627_K 1.216 0.88
451_G 537_L 1.216 0.88
283_Y 323_L 1.216 0.88
215_L 218_E 1.211 0.88
376_R 390_E 1.21 0.88
297_R 307_E 1.21 0.88
45_S 49_R 1.21 0.88
351_I 396_G 1.207 0.88
480_V 511_A 1.201 0.88
318_L 330_V 1.199 0.88
155_I 177_F 1.198 0.87
390_E 401_L 1.197 0.87
603_A 635_L 1.195 0.87
310_I 330_V 1.193 0.87
151_R 618_S 1.192 0.87
9_P 20_I 1.191 0.87
424_R 427_V 1.19 0.87
449_V 526_C 1.189 0.87
137_D 140_I 1.186 0.87
656_S 664_G 1.186 0.87
392_D 397_E 1.184 0.87
203_R 214_K 1.183 0.87
497_K 579_E 1.18 0.86
47_G 51_M 1.179 0.86
146_D 151_R 1.178 0.86
55_Q 58_Q 1.175 0.86
140_I 159_N 1.174 0.86
24_Y 27_R 1.173 0.86
509_L 541_A 1.173 0.86
467_R 477_Y 1.17 0.86
82_G 138_N 1.17 0.86
447_L 478_A 1.17 0.86
387_T 403_Q 1.167 0.86
81_N 138_N 1.165 0.85
596_S 599_D 1.164 0.85
414_Y 472_D 1.161 0.85
295_L 310_I 1.159 0.85
421_I 429_V 1.159 0.85
630_A 634_E 1.158 0.85
103_A 110_E 1.156 0.85
208_T 212_Q 1.154 0.85
423_A 506_N 1.151 0.84
529_M 553_I 1.147 0.84
24_Y 563_T 1.143 0.84
473_R 669_Y 1.141 0.84
180_A 230_K 1.141 0.84
613_T 622_Y 1.138 0.84
504_T 533_A 1.136 0.83
552_V 609_L 1.135 0.83
512_C 521_G 1.133 0.83
190_R 200_Q 1.133 0.83
444_H 525_L 1.13 0.83
237_V 254_A 1.129 0.83
100_R 111_W 1.128 0.83
555_Q 668_E 1.126 0.83
531_G 556_V 1.125 0.83
529_M 668_E 1.122 0.82
563_T 624_E 1.122 0.82
505_F 595_Y 1.118 0.82
45_S 48_H 1.117 0.82
483_R 507_D 1.115 0.82
70_P 405_E 1.11 0.81
533_A 536_M 1.109 0.81
157_F 179_W 1.104 0.81
308_E 311_P 1.102 0.81
514_A 517_K 1.102 0.81
513_D 547_E 1.097 0.80
317_M 486_G 1.097 0.80
11_A 20_I 1.094 0.80
559_V 628_W 1.094 0.80
134_I 160_L 1.094 0.80
666_A 670_A 1.094 0.80
5_A 43_E 1.091 0.80
15_H 586_P 1.09 0.80
283_Y 297_R 1.087 0.80
440_F 445_N 1.087 0.80
13_T 17_D 1.087 0.80
281_R 299_R 1.084 0.79
187_Y 216_I 1.084 0.79
64_E 68_R 1.083 0.79
493_Y 497_K 1.079 0.79
143_L 165_W 1.079 0.79
573_T 576_E 1.079 0.79
41_Q 52_A 1.077 0.79
624_E 627_K 1.077 0.79
509_L 548_L 1.075 0.78
248_E 267_R 1.074 0.78
636_K 640_H 1.071 0.78
184_W 205_A 1.069 0.78
436_H 439_H 1.068 0.78
119_K 122_A 1.066 0.78
49_R 52_A 1.065 0.78
329_V 393_M 1.061 0.77
158_R 163_G 1.06 0.77
63_K 67_D 1.058 0.77
33_Q 36_V 1.058 0.77
13_T 16_G 1.056 0.77
638_D 642_L 1.056 0.77
34_P 37_L 1.054 0.77
12_M 19_R 1.054 0.77
83_Y 112_E 1.053 0.76
554_A 559_V 1.052 0.76
296_Y 308_E 1.046 0.76
501_K 536_M 1.045 0.76
626_A 646_T 1.045 0.76
449_V 480_V 1.043 0.75
440_F 444_H 1.042 0.75
501_K 504_T 1.039 0.75
643_L 674_A 1.039 0.75
390_E 399_R 1.036 0.75
276_D 323_L 1.036 0.75
435_Y 439_H 1.034 0.75
572_L 576_E 1.032 0.74
231_T 236_Y 1.032 0.74
538_M 541_A 1.032 0.74
539_G 559_V 1.029 0.74
236_Y 282_F 1.028 0.74
400_V 403_Q 1.023 0.73
415_R 435_Y 1.021 0.73
650_S 660_K 1.021 0.73
434_V 479_I 1.02 0.73
8_I 35_E 1.019 0.73
280_H 299_R 1.018 0.73
467_R 471_L 1.018 0.73
418_H 432_S 1.017 0.73
421_I 510_D 1.017 0.73
154_G 169_L 1.017 0.73
421_I 427_V 1.017 0.73
478_A 511_A 1.016 0.73
48_H 58_Q 1.015 0.73
551_G 672_L 1.015 0.73
418_H 430_P 1.015 0.73
48_H 55_Q 1.014 0.73
63_K 66_I 1.012 0.72
290_G 296_Y 1.012 0.72
98_Y 117_A 1.012 0.72
156_R 165_W 1.011 0.72
28_D 32_S 1.006 0.72
556_V 619_Q 1.004 0.71
217_Y 250_R 1.004 0.71
588_Y 591_Y 1.003 0.71
516_L 523_P 1.001 0.71
391_L 398_R 1.001 0.71
287_N 293_F 1 0.71
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2xe4A10.99711000.237Contact Map0.727
3iujA10.97381000.286Contact Map0.739
1yr2A10.97521000.289Contact Map0.698
2bklA20.97381000.295Contact Map0.734
2xdwA10.97231000.314Contact Map0.736
4hvtA10.96791000.361Contact Map0.676
3azoA20.89361000.527Contact Map0.592
4hxfB10.8281000.597Contact Map0.732
4a5sA20.9141000.599Contact Map0.629
2z3zA10.8951000.606Contact Map0.646
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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