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OPENSEQ.org
PCKA - Phosphoenolpyruvate carboxykinase [ATP]
UniProt: P22259 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10688
Length: 540 (516)
Sequences: 805
Seq/Len: 1.56
PCKA
Paralog alert: 0.04 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
74_R 79_R 3.862 1.00
497_R 507_Q 3.769 1.00
294_K 302_N 3.578 1.00
519_D 522_D 3.536 1.00
39_P 119_F 3.432 1.00
237_V 351_A 3.381 1.00
371_V 462_I 3.148 1.00
512_T 516_L 3.136 1.00
427_K 431_A 3.028 1.00
291_K 324_D 2.982 1.00
238_G 242_D 2.863 1.00
190_F 203_G 2.635 1.00
122_D 136_R 2.566 1.00
502_S 505_Q 2.514 1.00
58_D 328_K 2.459 1.00
387_F 403_P 2.444 1.00
448_K 451_S 2.437 1.00
373_R 435_Q 2.431 1.00
132_R 159_E 2.41 1.00
473_F 495_D 2.322 1.00
130_D 276_D 2.31 0.99
471_E 484_T 2.259 0.99
474_T 481_A 2.199 0.99
353_H 432_A 2.189 0.99
376_A 430_Q 2.169 0.99
454_D 490_D 2.165 0.99
85_A 93_D 2.156 0.99
473_F 484_T 2.114 0.99
75_D 78_T 2.103 0.99
124_F 132_R 2.101 0.99
423_E 427_K 2.099 0.99
50_T 56_A 2.099 0.99
515_K 519_D 2.021 0.98
243_V 278_V 2.007 0.98
112_Q 175_C 1.998 0.98
151_M 335_S 1.981 0.98
34_Q 319_G 1.974 0.98
75_D 82_F 1.964 0.98
316_R 320_T 1.923 0.97
371_V 458_I 1.914 0.97
308_A 334_V 1.889 0.97
356_K 437_Y 1.872 0.97
134_S 166_D 1.863 0.97
498_N 503_P 1.846 0.97
522_D 525_T 1.83 0.96
24_V 117_R 1.812 0.96
509_K 512_T 1.81 0.96
103_Q 197_E 1.804 0.96
99_P 103_Q 1.803 0.96
81_T 225_L 1.794 0.96
39_P 117_R 1.792 0.96
34_Q 38_D 1.781 0.96
139_T 145_A 1.768 0.95
124_F 159_E 1.735 0.95
108_L 183_Q 1.728 0.95
106_K 197_E 1.723 0.94
475_L 482_I 1.722 0.94
131_T 226_K 1.714 0.94
75_D 196_T 1.686 0.94
62_F 189_N 1.684 0.93
51_N 320_T 1.679 0.93
458_I 462_I 1.67 0.93
50_T 315_V 1.67 0.93
262_K 460_D 1.647 0.92
76_D 79_R 1.638 0.92
156_S 159_E 1.629 0.92
218_M 222_L 1.621 0.92
293_S 296_A 1.613 0.91
47_G 57_V 1.609 0.91
466_S 470_A 1.595 0.91
194_N 197_E 1.593 0.91
36_E 55_V 1.59 0.91
98_S 101_T 1.558 0.89
52_L 323_F 1.554 0.89
450_I 493_I 1.552 0.89
125_C 215_M 1.538 0.89
362_A 443_W 1.533 0.88
485_E 491_T 1.531 0.88
151_M 309_L 1.529 0.88
507_Q 511_E 1.523 0.88
28_S 31_L 1.52 0.88
230_S 416_L 1.519 0.88
97_L 192_A 1.509 0.87
304_I 323_F 1.508 0.87
239_E 264_R 1.492 0.87
451_S 454_D 1.486 0.86
505_Q 508_E 1.477 0.86
30_D 34_Q 1.474 0.86
77_T 82_F 1.469 0.85
179_Q 182_E 1.459 0.85
124_F 156_S 1.455 0.85
37_L 49_L 1.452 0.84
289_T 332_T 1.441 0.84
144_Q 208_G 1.43 0.83
508_E 512_T 1.43 0.83
296_A 452_I 1.427 0.83
113_L 173_A 1.426 0.83
244_A 463_L 1.419 0.83
501_A 504_E 1.416 0.82
86_D 93_D 1.409 0.82
230_S 281_F 1.404 0.82
150_N 153_I 1.399 0.81
380_Q 426_V 1.393 0.81
99_P 196_T 1.392 0.81
289_T 334_V 1.392 0.81
148_V 152_F 1.381 0.80
109_V 173_A 1.377 0.80
26_N 122_D 1.373 0.80
51_N 321_I 1.367 0.79
491_T 498_N 1.358 0.79
109_V 203_G 1.358 0.79
208_G 309_L 1.358 0.79
122_D 165_P 1.356 0.78
359_F 436_A 1.353 0.78
444_N 450_I 1.352 0.78
474_T 479_N 1.348 0.78
30_D 319_G 1.345 0.78
154_R 339_Y 1.337 0.77
531_A 535_A 1.318 0.76
27_P 31_L 1.318 0.76
305_R 342_D 1.314 0.75
504_E 508_E 1.314 0.75
12_L 16_G 1.304 0.75
475_L 479_N 1.297 0.74
131_T 228_I 1.297 0.74
238_G 243_V 1.297 0.74
225_L 416_L 1.294 0.74
54_A 323_F 1.293 0.74
473_F 498_N 1.292 0.74
152_F 215_M 1.29 0.73
109_V 190_F 1.286 0.73
148_V 211_M 1.285 0.73
435_Q 468_D 1.281 0.73
140_E 175_C 1.281 0.73
385_S 520_N 1.28 0.73
160_L 163_F 1.279 0.73
77_T 196_T 1.271 0.72
193_F 217_S 1.269 0.72
338_I 349_S 1.269 0.72
57_V 144_Q 1.263 0.71
97_L 101_T 1.257 0.71
362_A 391_L 1.251 0.70
34_Q 306_R 1.245 0.70
62_F 396_R 1.245 0.70
217_S 415_S 1.245 0.70
310_L 334_V 1.238 0.69
178_P 203_G 1.235 0.69
123_A 163_F 1.227 0.68
151_M 289_T 1.217 0.67
361_T 382_H 1.211 0.67
56_A 315_V 1.206 0.66
299_E 342_D 1.203 0.66
33_Y 49_L 1.201 0.66
76_D 82_F 1.201 0.66
157_D 161_A 1.194 0.65
26_N 136_R 1.183 0.64
12_L 17_I 1.176 0.63
133_L 222_L 1.176 0.63
121_V 149_K 1.173 0.63
167_F 198_R 1.17 0.63
30_D 306_R 1.169 0.63
277_G 353_H 1.159 0.62
135_V 163_F 1.158 0.61
451_S 458_I 1.157 0.61
151_M 214_G 1.152 0.61
471_E 485_E 1.152 0.61
108_L 182_E 1.149 0.61
130_D 227_G 1.147 0.60
76_D 196_T 1.143 0.60
35_E 38_D 1.139 0.60
97_L 185_L 1.137 0.59
83_W 418_P 1.136 0.59
169_V 215_M 1.135 0.59
422_A 537_G 1.125 0.58
112_Q 140_E 1.123 0.58
470_A 485_E 1.122 0.58
377_D 532_A 1.12 0.58
36_E 345_V 1.118 0.57
495_D 498_N 1.117 0.57
372_S 382_H 1.114 0.57
385_S 407_A 1.113 0.57
215_M 218_M 1.111 0.57
291_K 296_A 1.105 0.56
219_M 281_F 1.104 0.56
111_R 114_S 1.101 0.56
116_K 140_E 1.1 0.56
315_V 321_I 1.095 0.55
71_Y 101_T 1.094 0.55
275_D 431_A 1.091 0.55
75_D 194_N 1.088 0.54
70_K 414_L 1.087 0.54
243_V 351_A 1.086 0.54
439_V 462_I 1.086 0.54
437_Y 537_G 1.085 0.54
170_M 480_L 1.085 0.54
444_N 448_K 1.085 0.54
120_V 138_I 1.081 0.54
400_E 512_T 1.077 0.53
375_T 481_A 1.076 0.53
102_W 446_T 1.076 0.53
299_E 302_N 1.071 0.53
24_V 27_P 1.068 0.52
71_Y 192_A 1.067 0.52
166_D 198_R 1.064 0.52
121_V 145_A 1.062 0.52
109_V 201_L 1.057 0.51
216_F 220_N 1.056 0.51
80_D 358_I 1.055 0.51
180_W 185_L 1.054 0.51
467_L 470_A 1.054 0.51
246_F 257_L 1.051 0.50
154_R 338_I 1.048 0.50
195_L 412_A 1.04 0.49
477_M 497_R 1.036 0.49
143_W 384_L 1.034 0.49
48_V 315_V 1.032 0.48
13_E 19_D 1.031 0.48
136_R 165_P 1.03 0.48
457_A 461_A 1.029 0.48
531_A 534_V 1.028 0.48
29_Y 149_K 1.028 0.48
27_P 121_V 1.027 0.48
68_K 86_D 1.026 0.48
223_L 272_G 1.022 0.47
67_P 261_P 1.022 0.47
83_W 459_I 1.022 0.47
74_R 96_P 1.02 0.47
113_L 118_L 1.015 0.47
124_F 160_L 1.014 0.47
264_R 346_K 1.012 0.46
152_F 190_F 1.012 0.46
482_I 534_V 1.009 0.46
82_F 196_T 1.008 0.46
364_A 401_P 1.005 0.46
47_G 55_V 1.003 0.45
335_S 390_K 1.002 0.45
259_T 382_H 1.002 0.45
125_C 281_F 1 0.45
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1j3bA20.95931000.201Contact Map0.677
2olrA10.99441000.225Contact Map0.618
1ytmA20.97961000.233Contact Map0.632
1ii2A20.93891000.241Contact Map0.667
2zciA40.91671000.49Contact Map0.336
2fafA20.92591000.505Contact Map0.322
4gmuA10.92411000.56Contact Map0.314
3tqfA20.303791.20.976Contact Map0.234
1xjcA10.253788.80.978Contact Map0.261
1rz3A10.338984.30.979Contact Map0.219
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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