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OPENSEQ.org
PNCB - Nicotinate phosphoribosyltransferase
UniProt: P18133 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10742
Length: 400 (384)
Sequences: 1342
Seq/Len: 3.49
PNCB
Paralog alert: 0.06 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
280_L 294_A 5.476 1.00
207_A 214_P 4.347 1.00
75_S 82_A 3.915 1.00
68_D 89_R 3.379 1.00
39_F 125_L 3.186 1.00
200_G 215_M 2.936 1.00
95_P 113_G 2.896 1.00
236_S 239_A 2.884 1.00
257_T 294_A 2.756 1.00
296_A 300_K 2.743 1.00
113_G 118_V 2.734 1.00
25_A 222_W 2.677 1.00
28_H 81_K 2.64 1.00
238_R 242_A 2.594 1.00
57_Q 125_L 2.486 1.00
293_K 296_A 2.366 1.00
61_M 97_Q 2.362 1.00
254_I 279_G 2.353 1.00
68_D 72_Q 2.33 1.00
270_G 303_I 2.32 1.00
67_Q 70_E 2.296 1.00
257_T 280_L 2.251 1.00
257_T 297_H 2.217 1.00
114_P 117_E 2.209 1.00
205_D 208_R 2.197 1.00
109_I 129_I 2.186 1.00
23_Q 84_Y 2.122 1.00
34_H 112_S 2.098 1.00
17_A 77_L 2.084 1.00
23_Q 64_L 2.043 1.00
189_V 206_L 2.026 1.00
36_A 112_S 2.004 1.00
242_A 246_E 2.002 1.00
172_M 200_G 1.968 1.00
71_Y 85_L 1.961 1.00
39_F 57_Q 1.921 1.00
265_F 268_D 1.899 1.00
70_E 208_R 1.856 0.99
321_A 338_I 1.847 0.99
77_L 214_P 1.846 0.99
94_N 117_E 1.8 0.99
57_Q 109_I 1.796 0.99
82_A 86_N 1.759 0.99
169_F 325_Y 1.747 0.99
61_M 121_W 1.738 0.99
24_Q 81_K 1.726 0.99
186_E 209_R 1.707 0.99
158_S 171_L 1.706 0.99
26_V 119_I 1.697 0.99
172_M 215_M 1.69 0.99
158_S 199_V 1.67 0.99
170_H 199_V 1.652 0.99
65_R 92_R 1.64 0.98
20_L 80_F 1.626 0.98
325_Y 336_F 1.624 0.98
111_L 356_I 1.621 0.98
171_L 198_F 1.616 0.98
280_L 297_H 1.609 0.98
29_H 243_A 1.568 0.98
320_K 323_E 1.559 0.98
264_A 267_R 1.557 0.98
176_T 203_N 1.548 0.97
57_Q 124_P 1.541 0.97
28_H 83_D 1.539 0.97
263_D 293_K 1.533 0.97
226_H 243_A 1.529 0.97
86_N 89_R 1.529 0.97
60_A 124_P 1.513 0.97
294_A 297_H 1.509 0.97
87_W 116_R 1.503 0.97
309_T 334_L 1.502 0.97
95_P 117_E 1.501 0.97
75_S 89_R 1.481 0.96
86_N 90_E 1.477 0.96
69_D 208_R 1.469 0.96
267_R 271_V 1.452 0.96
39_F 129_I 1.444 0.96
64_L 91_F 1.437 0.96
22_M 219_A 1.423 0.95
73_W 77_L 1.418 0.95
24_Q 248_Y 1.413 0.95
311_V 335_S 1.411 0.95
35_V 115_W 1.407 0.95
266_L 294_A 1.397 0.95
26_V 115_W 1.389 0.94
176_T 188_I 1.386 0.94
238_R 268_D 1.379 0.94
288_V 327_H 1.368 0.94
257_T 266_L 1.365 0.94
238_R 271_V 1.358 0.93
23_Q 116_R 1.353 0.93
73_W 76_A 1.349 0.93
73_W 214_P 1.338 0.93
340_T 351_P 1.336 0.93
169_F 336_F 1.33 0.93
267_R 297_H 1.325 0.92
264_A 268_D 1.322 0.92
295_I 299_E 1.32 0.92
324_L 331_R 1.307 0.92
341_R 346_I 1.297 0.91
50_Y 155_V 1.293 0.91
72_Q 75_S 1.287 0.91
191_R 343_T 1.256 0.89
239_A 243_A 1.253 0.89
201_T 212_L 1.251 0.89
93_F 113_G 1.248 0.89
94_N 113_G 1.248 0.89
291_G 312_F 1.244 0.89
113_G 121_W 1.237 0.88
17_A 204_Y 1.235 0.88
73_W 207_A 1.232 0.88
24_Q 28_H 1.231 0.88
319_R 323_E 1.223 0.88
287_P 333_Q 1.223 0.88
41_C 314_D 1.217 0.87
215_M 335_S 1.202 0.86
29_H 246_E 1.201 0.86
72_Q 76_A 1.192 0.86
17_A 214_P 1.188 0.86
66_L 71_Y 1.186 0.85
276_R 305_P 1.185 0.85
322_V 326_R 1.184 0.85
295_I 332_V 1.173 0.85
121_W 125_L 1.168 0.84
265_F 272_E 1.167 0.84
113_G 282_H 1.16 0.84
315_N 353_N 1.155 0.83
205_D 209_R 1.155 0.83
14_D 176_T 1.141 0.82
55_R 59_Q 1.136 0.82
92_R 117_E 1.119 0.81
121_W 326_R 1.118 0.81
21_H 80_F 1.117 0.80
13_L 74_L 1.112 0.80
23_Q 120_L 1.102 0.79
315_N 351_P 1.102 0.79
73_W 208_R 1.094 0.79
263_D 267_R 1.092 0.78
91_F 117_E 1.083 0.78
61_M 95_P 1.08 0.77
263_D 268_D 1.078 0.77
268_D 272_E 1.072 0.77
338_I 369_L 1.068 0.76
201_T 207_A 1.066 0.76
64_L 121_W 1.066 0.76
25_A 248_Y 1.065 0.76
38_E 110_R 1.061 0.75
153_K 345_D 1.061 0.75
26_V 35_V 1.061 0.75
218_Q 223_F 1.054 0.75
61_M 124_P 1.047 0.74
50_Y 159_A 1.044 0.74
172_M 335_S 1.034 0.73
59_Q 63_H 1.033 0.73
224_Q 254_I 1.031 0.72
158_S 168_R 1.029 0.72
206_L 212_L 1.028 0.72
24_Q 247_E 1.023 0.72
355_V 370_S 1.021 0.71
190_K 210_L 1.013 0.71
303_I 308_K 1.012 0.70
279_G 308_K 1.01 0.70
175_G 179_R 1.005 0.70
156_D 318_L 1.004 0.70
175_G 343_T 1.001 0.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3os4A211000.15Contact Map0.629
4hl7A20.99751000.158Contact Map0.577
1yirA411000.169Contact Map0.591
2im5A40.95251000.181Contact Map0.617
1vlpA40.981000.19Contact Map0.591
1ybeA20.97251000.2Contact Map0.601
4mzyA10.91000.346Contact Map0.613
2f7fA10.91000.346Contact Map0.629
4kfnA20.91251000.353Contact Map0.461
4lvfA20.91251000.353Contact Map0.46
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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