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OPENSEQ.org
NFSA - Oxygen-insensitive NADPH nitroreductase
UniProt: P17117 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11261
Length: 240 (240)
Sequences: 636
Seq/Len: 2.65
NFSA
Paralog alert: 0.06 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
52_T 179_N 3.677 1.00
80_C 149_V 3.253 1.00
116_L 126_G 3.246 1.00
60_L 139_V 2.983 1.00
81_A 104_G 2.953 1.00
8_I 117_I 2.5 1.00
23_S 26_Q 2.483 1.00
20_E 159_W 2.404 1.00
78_V 143_L 2.396 1.00
116_L 155_L 2.392 1.00
14_I 164_P 2.312 1.00
50_R 75_E 2.302 1.00
30_I 115_A 2.128 1.00
137_E 234_K 2.119 1.00
193_D 213_S 2.096 1.00
38_S 126_G 2.05 1.00
58_E 71_A 2.042 1.00
56_L 179_N 1.983 0.99
112_A 128_Y 1.97 0.99
229_L 233_H 1.949 0.99
189_L 213_S 1.933 0.99
63_L 135_N 1.851 0.99
135_N 138_A 1.828 0.99
52_T 178_E 1.799 0.99
26_Q 124_L 1.761 0.98
26_Q 30_I 1.729 0.98
17_F 156_C 1.728 0.98
55_A 58_E 1.726 0.98
134_N 225_R 1.698 0.98
77_W 115_A 1.694 0.98
127_V 156_C 1.69 0.98
140_T 235_Q 1.687 0.98
63_L 138_A 1.686 0.98
76_F 129_I 1.684 0.98
32_N 35_R 1.676 0.98
132_L 139_V 1.66 0.97
144_K 180_S 1.643 0.97
112_A 153_F 1.628 0.97
18_T 161_A 1.626 0.97
124_L 159_W 1.597 0.96
2_T 121_S 1.58 0.96
140_T 231_Y 1.563 0.96
50_R 176_V 1.561 0.96
59_E 62_T 1.551 0.96
54_K 58_E 1.544 0.96
3_P 6_E 1.536 0.95
48_I 79_F 1.514 0.95
69_H 162_D 1.464 0.94
53_D 179_N 1.439 0.93
79_F 115_A 1.391 0.91
22_I 74_A 1.384 0.91
109_A 153_F 1.344 0.89
211_T 214_D 1.32 0.88
105_V 150_L 1.316 0.88
27_R 77_W 1.313 0.88
22_I 157_L 1.311 0.87
129_I 156_C 1.307 0.87
101_L 232_L 1.303 0.87
199_Y 203_R 1.292 0.86
107_D 130_G 1.29 0.86
28_E 172_A 1.278 0.86
51_I 60_L 1.251 0.84
37_T 110_M 1.231 0.83
77_W 157_L 1.219 0.82
105_V 152_L 1.215 0.81
102_L 105_V 1.212 0.81
31_I 176_V 1.204 0.81
26_Q 122_L 1.199 0.80
18_T 159_W 1.198 0.80
99_E 106_V 1.197 0.80
62_T 66_G 1.194 0.80
26_Q 159_W 1.191 0.80
59_E 142_L 1.179 0.79
27_R 31_I 1.178 0.79
170_L 175_L 1.171 0.78
11_H 114_N 1.169 0.78
84_N 88_Q 1.167 0.78
48_I 176_V 1.163 0.77
113_Q 128_Y 1.14 0.75
48_I 77_W 1.136 0.75
101_L 104_G 1.131 0.74
78_V 132_L 1.128 0.74
56_L 180_S 1.128 0.74
12_R 164_P 1.126 0.74
132_L 151_P 1.117 0.73
195_Q 218_R 1.101 0.72
34_A 115_A 1.088 0.70
28_E 173_S 1.085 0.70
24_E 52_T 1.08 0.70
4_T 121_S 1.068 0.68
74_A 237_W 1.068 0.68
210_D 215_H 1.053 0.67
42_F 192_Y 1.051 0.67
147_Q 235_Q 1.051 0.67
46_S 109_A 1.048 0.66
2_T 5_I 1.048 0.66
9_C 29_A 1.042 0.66
76_F 154_G 1.042 0.66
46_S 111_M 1.035 0.65
229_L 238_A 1.021 0.63
23_S 159_W 1.02 0.63
30_I 200_Y 1.017 0.63
63_L 131_G 1.014 0.62
17_F 69_H 1.014 0.62
76_F 131_G 1.011 0.62
216_I 222_K 1.009 0.62
16_H 161_A 1.007 0.62
26_Q 115_A 1 0.61
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1f5vA211000.22Contact Map0.821
1bkjA211000.22Contact Map0.797
1zchA111000.225Contact Map0.685
3n2sA411000.225Contact Map0.833
3eofA20.95831000.331Contact Map0.835
3bemA20.73751000.5Contact Map0.756
3e39A20.71671000.5Contact Map0.711
1noxA10.73751000.501Contact Map0.705
4dn2A20.71671000.503Contact Map0.727
3gagA40.74171000.504Contact Map0.736
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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