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OPENSEQ.org
PHND - Phosphonates-binding periplasmic protein
UniProt: P16682 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10714
Length: 338 (299)
Sequences: 492
Seq/Len: 1.65
PHND
Paralog alert: 0.09 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
132_N 220_K 3.446 1.00
51_T 55_Q 2.826 1.00
105_Q 244_K 2.35 1.00
124_I 190_V 2.339 1.00
186_N 198_A 2.256 0.99
125_V 220_K 2.183 0.99
17_L 20_L 2.128 0.99
137_L 197_V 2.057 0.99
190_V 198_A 2.032 0.99
276_S 279_Q 1.829 0.97
241_E 245_D 1.823 0.97
248_Y 274_A 1.822 0.97
277_D 284_R 1.779 0.96
60_K 253_N 1.779 0.96
126_N 193_K 1.738 0.95
95_A 99_V 1.734 0.95
275_S 279_Q 1.684 0.94
81_R 102_A 1.681 0.94
91_G 154_T 1.659 0.93
120_W 224_K 1.635 0.93
4_K 8_S 1.62 0.92
61_L 65_V 1.616 0.92
171_S 174_D 1.6 0.92
128_D 220_K 1.548 0.90
95_A 277_D 1.523 0.89
32_A 66_N 1.505 0.88
106_V 275_S 1.476 0.87
36_G 85_V 1.471 0.87
124_I 187_A 1.463 0.86
148_N 179_V 1.46 0.86
119_Y 150_D 1.451 0.86
16_S 22_S 1.424 0.84
95_A 280_L 1.417 0.84
130_P 220_K 1.406 0.83
135_N 249_D 1.404 0.83
107_F 235_W 1.397 0.83
57_M 87_I 1.397 0.83
245_D 249_D 1.379 0.81
32_A 63_V 1.367 0.81
118_G 227_L 1.363 0.80
64_K 278_L 1.361 0.80
123_L 197_V 1.346 0.79
148_N 199_T 1.343 0.79
147_G 189_A 1.342 0.79
5_I 8_S 1.338 0.79
146_F 175_F 1.329 0.78
136_D 171_S 1.317 0.77
56_D 254_Y 1.315 0.77
146_F 197_V 1.312 0.77
16_S 24_A 1.311 0.77
243_T 247_I 1.287 0.75
153_S 183_H 1.286 0.75
157_F 161_G 1.279 0.74
115_G 119_Y 1.279 0.74
155_S 283_I 1.273 0.74
57_M 250_F 1.263 0.73
217_K 265_E 1.262 0.73
120_W 202_T 1.262 0.73
252_M 274_A 1.255 0.72
7_A 10_A 1.254 0.72
236_R 240_S 1.249 0.72
58_E 64_K 1.247 0.71
6_I 10_A 1.244 0.71
76_I 85_V 1.235 0.70
159_V 228_I 1.229 0.70
23_P 26_A 1.223 0.69
168_N 197_V 1.218 0.69
119_Y 155_S 1.215 0.69
72_D 75_G 1.213 0.68
123_L 199_T 1.211 0.68
9_L 12_T 1.208 0.68
18_S 23_P 1.205 0.68
19_T 25_H 1.204 0.68
83_N 238_N 1.201 0.67
245_D 248_Y 1.194 0.67
18_S 220_K 1.186 0.66
295_V 299_K 1.186 0.66
87_I 239_L 1.176 0.65
202_T 224_K 1.175 0.65
95_A 232_P 1.166 0.64
6_I 9_L 1.161 0.63
156_G 183_H 1.158 0.63
262_A 287_A 1.156 0.63
142_K 246_K 1.149 0.62
133_N 218_E 1.146 0.62
54_L 225_S 1.146 0.62
6_I 12_T 1.145 0.62
91_G 94_S 1.144 0.62
187_A 208_L 1.133 0.61
33_L 86_D 1.129 0.60
4_K 10_A 1.128 0.60
19_T 22_S 1.123 0.60
141_R 144_L 1.116 0.59
21_L 24_A 1.115 0.59
3_A 7_A 1.108 0.58
122_V 224_K 1.106 0.58
3_A 9_L 1.105 0.58
278_L 281_V 1.105 0.58
24_A 28_E 1.102 0.57
64_K 248_Y 1.099 0.57
99_V 284_R 1.092 0.56
167_K 263_V 1.087 0.56
149_G 186_N 1.083 0.55
61_L 64_K 1.082 0.55
18_S 22_S 1.076 0.55
127_K 220_K 1.075 0.55
140_K 143_D 1.075 0.55
33_L 219_L 1.068 0.54
109_Q 280_L 1.065 0.54
147_G 180_N 1.064 0.53
80_M 102_A 1.062 0.53
189_A 195_V 1.061 0.53
7_A 11_F 1.059 0.53
119_Y 153_S 1.058 0.53
15_F 24_A 1.056 0.53
5_I 9_L 1.05 0.52
77_I 98_A 1.05 0.52
3_A 6_I 1.043 0.51
163_Y 223_W 1.041 0.51
55_Q 58_E 1.039 0.51
281_V 284_R 1.036 0.51
187_A 190_V 1.036 0.51
205_L 230_G 1.034 0.50
98_A 160_P 1.034 0.50
122_V 202_T 1.033 0.50
62_G 246_K 1.033 0.50
5_I 10_A 1.03 0.50
164_V 197_V 1.03 0.50
35_F 233_I 1.027 0.50
153_S 156_G 1.023 0.49
248_Y 252_M 1.021 0.49
62_G 121_S 1.021 0.49
4_K 7_A 1.019 0.49
124_I 198_A 1.018 0.49
135_N 281_V 1.017 0.49
18_S 29_Q 1.005 0.47
155_S 251_F 1.003 0.47
162_Y 167_K 1.003 0.47
18_S 21_L 1.002 0.47
255_G 271_P 1.002 0.47
4_K 9_L 1.001 0.47
18_S 24_A 1 0.47
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ekiA10.84021000.375Contact Map0.366
3myuA20.74561000.43Contact Map0.385
3p7iA10.94971000.527Contact Map0.65
3n5lA20.91121000.559Contact Map0.607
1blfA10.8581000.614Contact Map0.534
1iejA10.74851000.62Contact Map0.611
3v83A60.93491000.628Contact Map0.62
1ryoA10.75741000.641Contact Map0.574
1h45A10.76331000.647Contact Map0.61
1cb6A10.8581000.656Contact Map0.557
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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