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OPENSEQ.org
SYP - Proline--tRNA ligase
UniProt: P16659 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10770
Length: 572 (570)
Sequences: 1866
Seq/Len: 3.27
SYP
Paralog alert: 0.10 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
254_L 365_I 5.778 1.00
186_S 192_F 4.491 1.00
256_D 368_K 3.392 1.00
51_K 54_E 3.105 1.00
495_K 499_E 3.04 1.00
82_E 119_R 2.996 1.00
543_D 556_L 2.808 1.00
164_H 169_S 2.793 1.00
164_H 173_T 2.763 1.00
256_D 367_G 2.645 1.00
227_S 396_S 2.631 1.00
63_N 183_K 2.607 1.00
57_V 448_V 2.6 1.00
69_V 114_I 2.549 1.00
264_A 268_E 2.539 1.00
561_D 564_E 2.499 1.00
224_S 227_S 2.494 1.00
179_A 183_K 2.478 1.00
62_N 133_Y 2.452 1.00
543_D 558_K 2.395 1.00
455_A 471_I 2.389 1.00
565_Y 568_K 2.379 1.00
491_E 495_K 2.374 1.00
456_I 465_I 2.329 1.00
77_A 81_Q 2.299 1.00
171_Q 416_Q 2.296 1.00
419_T 423_E 2.285 1.00
253_T 371_F 2.25 1.00
548_Y 551_N 2.241 1.00
555_Q 565_Y 2.229 1.00
224_S 396_S 2.158 1.00
558_K 561_D 2.142 1.00
475_Q 506_E 2.103 1.00
59_E 187_R 2.098 1.00
97_V 103_P 2.078 1.00
171_Q 175_D 2.077 1.00
488_R 540_D 2.073 1.00
254_L 368_K 2.056 1.00
122_L 130_L 2.037 1.00
270_F 360_A 2.006 1.00
26_M 149_V 1.996 1.00
107_G 110_H 1.994 1.00
58_R 68_E 1.976 1.00
6_Y 67_I 1.964 1.00
166_S 169_S 1.954 1.00
280_T 295_A 1.954 1.00
169_S 173_T 1.954 1.00
478_I 509_L 1.92 1.00
392_A 404_L 1.917 1.00
74_V 96_F 1.888 0.99
110_H 134_Q 1.885 0.99
64_A 131_N 1.873 0.99
55_N 59_E 1.865 0.99
66_A 131_N 1.854 0.99
14_T 24_Q 1.849 0.99
564_E 568_K 1.844 0.99
282_L 295_A 1.839 0.99
297_L 349_I 1.838 0.99
361_A 373_I 1.838 0.99
53_V 452_V 1.812 0.99
81_Q 87_E 1.799 0.99
152_S 449_T 1.796 0.99
160_A 177_M 1.79 0.99
476_V 505_I 1.786 0.99
530_H 546_Y 1.784 0.99
508_L 528_I 1.779 0.99
334_G 366_D 1.775 0.99
175_D 179_A 1.763 0.99
131_N 160_A 1.762 0.99
353_V 356_M 1.756 0.99
6_Y 474_F 1.749 0.99
278_V 359_F 1.748 0.99
254_L 370_Y 1.736 0.99
172_E 176_A 1.721 0.99
358_D 372_G 1.717 0.99
2_R 494_E 1.716 0.99
250_Q 253_T 1.693 0.99
365_I 368_K 1.693 0.99
396_S 399_G 1.687 0.99
477_A 523_M 1.681 0.99
323_T 326_E 1.674 0.98
272_L 360_A 1.67 0.98
78_D 81_Q 1.659 0.98
69_V 117_L 1.648 0.98
170_L 416_Q 1.646 0.98
253_T 372_G 1.634 0.98
88_Q 420_K 1.618 0.98
165_T 429_V 1.617 0.98
358_D 374_N 1.614 0.98
503_Q 567_V 1.586 0.98
47_V 51_K 1.583 0.98
178_Y 208_H 1.582 0.98
26_M 31_M 1.577 0.97
260_A 266_L 1.576 0.97
494_E 498_S 1.575 0.97
337_S 366_D 1.564 0.97
337_S 367_G 1.559 0.97
469_D 475_Q 1.542 0.97
516_P 520_F 1.533 0.97
19_E 23_H 1.527 0.97
302_H 352_T 1.517 0.97
532_I 562_I 1.514 0.97
492_L 495_K 1.499 0.96
227_S 399_G 1.493 0.96
92_E 95_R 1.491 0.96
225_D 404_L 1.488 0.96
492_L 539_L 1.482 0.96
52_K 506_E 1.482 0.96
463_R 521_A 1.463 0.96
476_V 532_I 1.46 0.96
86_W 105_V 1.46 0.96
477_A 528_I 1.459 0.96
478_I 496_L 1.457 0.95
207_S 413_H 1.455 0.95
356_M 359_F 1.454 0.95
390_V 403_L 1.452 0.95
428_S 438_I 1.45 0.95
476_V 500_L 1.446 0.95
348_V 387_I 1.445 0.95
561_D 565_Y 1.438 0.95
174_Y 208_H 1.434 0.95
487_F 491_E 1.408 0.94
48_R 506_E 1.397 0.94
265_E 268_E 1.394 0.94
53_V 471_I 1.394 0.94
252_M 370_Y 1.391 0.94
419_T 440_T 1.389 0.94
530_H 548_Y 1.389 0.94
173_T 176_A 1.388 0.94
428_S 436_N 1.383 0.94
167_Q 440_T 1.383 0.94
55_N 62_N 1.382 0.93
57_V 449_T 1.366 0.93
284_K 293_Q 1.359 0.93
304_L 309_A 1.349 0.92
327_I 335_P 1.349 0.92
220_D 405_I 1.344 0.92
270_F 371_F 1.343 0.92
531_T 554_K 1.339 0.92
50_L 449_T 1.331 0.92
333_A 338_L 1.331 0.92
118_I 132_F 1.328 0.92
297_L 353_V 1.328 0.92
530_H 566_L 1.318 0.91
423_E 438_I 1.317 0.91
359_F 375_W 1.316 0.91
285_A 319_L 1.313 0.91
497_Y 501_R 1.313 0.91
320_T 326_E 1.312 0.91
296_L 387_I 1.308 0.91
115_T 427_A 1.307 0.91
423_E 426_K 1.306 0.91
370_Y 373_I 1.303 0.91
97_V 101_E 1.301 0.90
396_S 400_Q 1.294 0.90
252_M 373_I 1.294 0.90
74_V 94_L 1.293 0.90
427_A 439_L 1.287 0.90
420_K 424_A 1.283 0.90
178_Y 182_S 1.277 0.89
263_I 266_L 1.275 0.89
416_Q 440_T 1.274 0.89
227_S 398_D 1.268 0.89
563_V 567_V 1.262 0.89
296_L 309_A 1.26 0.89
166_S 172_E 1.256 0.88
169_S 172_E 1.256 0.88
297_L 347_V 1.253 0.88
499_E 559_T 1.253 0.88
429_V 439_L 1.25 0.88
499_E 502_A 1.248 0.88
255_V 266_L 1.241 0.88
59_E 63_N 1.238 0.87
50_L 156_L 1.232 0.87
260_A 269_Q 1.228 0.87
223_F 404_L 1.223 0.86
492_L 538_N 1.218 0.86
256_D 365_I 1.211 0.86
73_V 139_F 1.21 0.86
64_A 176_A 1.207 0.85
283_V 306_E 1.203 0.85
284_K 326_E 1.202 0.85
60_E 187_R 1.201 0.85
546_Y 565_Y 1.196 0.85
252_M 341_V 1.193 0.85
219_D 232_N 1.189 0.84
488_R 491_E 1.188 0.84
64_A 180_A 1.187 0.84
64_A 177_M 1.184 0.84
348_V 385_A 1.18 0.84
263_I 274_I 1.176 0.83
237_E 351_R 1.171 0.83
224_S 399_G 1.17 0.83
469_D 530_H 1.167 0.83
354_A 384_V 1.165 0.83
267_V 277_T 1.157 0.82
514_E 518_V 1.156 0.82
227_S 400_Q 1.154 0.82
429_V 437_Q 1.154 0.82
26_M 456_I 1.15 0.82
83_S 89_Y 1.148 0.81
284_K 320_T 1.145 0.81
266_L 270_F 1.143 0.81
430_Q 435_R 1.141 0.81
81_Q 103_P 1.141 0.81
3_T 8_L 1.141 0.81
56_I 188_M 1.135 0.80
461_D 464_G 1.135 0.80
497_Y 509_L 1.134 0.80
222_V 403_L 1.132 0.80
478_I 493_A 1.131 0.80
295_A 343_M 1.13 0.80
481_M 535_G 1.127 0.80
537_R 541_N 1.126 0.80
480_P 509_L 1.124 0.79
480_P 490_Q 1.123 0.79
223_F 406_K 1.122 0.79
495_K 559_T 1.122 0.79
495_K 560_G 1.122 0.79
555_Q 569_Q 1.121 0.79
422_S 439_L 1.12 0.79
175_D 178_Y 1.116 0.79
349_I 353_V 1.113 0.78
419_T 438_I 1.111 0.78
263_I 277_T 1.11 0.78
182_S 194_A 1.109 0.78
431_G 437_Q 1.106 0.78
260_A 265_E 1.106 0.78
360_A 371_F 1.105 0.78
43_L 117_L 1.104 0.78
255_V 270_F 1.098 0.77
423_E 428_S 1.097 0.77
489_V 539_L 1.096 0.77
182_S 192_F 1.095 0.77
12_K 518_V 1.093 0.77
71_M 114_I 1.092 0.77
349_I 381_T 1.092 0.77
431_G 436_N 1.09 0.76
478_I 497_Y 1.089 0.76
498_S 502_A 1.081 0.76
537_R 556_L 1.076 0.75
61_M 158_K 1.074 0.75
489_V 492_L 1.073 0.75
469_D 548_Y 1.073 0.75
475_Q 505_I 1.072 0.75
478_I 507_V 1.068 0.74
52_K 55_N 1.067 0.74
282_L 338_L 1.066 0.74
257_T 369_H 1.061 0.74
51_K 55_N 1.06 0.74
21_I 460_Y 1.06 0.74
281_L 304_L 1.055 0.73
259_N 367_G 1.054 0.73
267_V 274_I 1.051 0.73
138_K 157_M 1.051 0.73
483_M 494_E 1.049 0.72
118_I 122_L 1.048 0.72
35_L 41_T 1.047 0.72
512_R 522_D 1.046 0.72
349_I 384_V 1.045 0.72
58_R 62_N 1.043 0.72
422_S 427_A 1.043 0.72
197_A 413_H 1.04 0.72
505_I 567_V 1.036 0.71
546_Y 557_I 1.033 0.71
328_R 334_G 1.029 0.70
250_Q 372_G 1.029 0.70
73_V 106_L 1.028 0.70
49_V 452_V 1.028 0.70
202_I 443_C 1.028 0.70
341_V 370_Y 1.026 0.70
544_I 557_I 1.024 0.70
459_N 550_R 1.023 0.70
31_M 465_I 1.021 0.70
194_A 413_H 1.02 0.69
21_I 24_Q 1.019 0.69
283_V 304_L 1.019 0.69
256_D 259_N 1.016 0.69
263_I 267_V 1.015 0.69
91_P 143_V 1.014 0.69
501_R 506_E 1.012 0.69
541_N 556_L 1.01 0.68
461_D 549_R 1.007 0.68
478_I 500_L 1.006 0.68
324_E 328_R 1.005 0.68
185_F 446_I 1.005 0.68
379_V 382_P 1.002 0.67
285_A 313_P 1.002 0.67
178_Y 414_I 1 0.67
14_T 27_L 1 0.67
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2j3lA20.98781000.308Contact Map0.824
2i4lA30.76571000.446Contact Map0.728
2zt5A10.87761000.515Contact Map0.515
4hvcA20.66961000.586Contact Map0.629
1qf6A10.7011000.599Contact Map0.566
4g85A20.82871000.6Contact Map0.52
1nj8A40.65031000.605Contact Map0.677
3ialA20.66431000.605Contact Map0.6
4g84A20.7921000.61Contact Map0.556
1nj1A10.66961000.61Contact Map0.636
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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