May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
MIAA - tRNA dimethylallyltransferase
UniProt: P16384 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10595
Length: 316 (296)
Sequences: 2229
Seq/Len: 7.53
MIAA
Paralog alert: 0.04 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
32_K 64_A 3.799 1.00
79_Y 87_D 3.654 1.00
219_H 272_V 3.48 1.00
152_V 175_F 3.105 1.00
228_A 231_R 2.975 1.00
263_S 266_E 2.866 1.00
136_Q 148_Q 2.809 1.00
227_E 254_M 2.8 1.00
37_E 68_R 2.78 1.00
94_D 98_A 2.726 1.00
132_R 176_F 2.651 1.00
219_H 268_V 2.572 1.00
256_S 261_E 2.538 1.00
20_A 214_I 2.452 1.00
212_Q 216_Q 2.425 1.00
136_Q 140_Q 2.413 1.00
215_E 272_V 2.319 1.00
222_L 268_V 2.25 1.00
148_Q 151_E 2.134 1.00
56_K 71_D 2.103 1.00
147_R 150_Q 2.079 1.00
68_R 98_A 2.073 1.00
264_Y 267_M 2.045 1.00
58_N 61_E 2.032 1.00
254_M 267_M 2.032 1.00
270_R 273_C 2.021 1.00
234_F 255_W 1.931 1.00
85_R 89_L 1.895 1.00
274_A 277_Q 1.873 1.00
37_E 98_A 1.865 1.00
95_I 98_A 1.853 1.00
104_L 115_L 1.85 1.00
48_K 74_D 1.839 1.00
156_A 160_I 1.836 1.00
174_V 182_L 1.833 1.00
51_D 56_K 1.803 1.00
24_T 27_A 1.778 1.00
273_C 277_Q 1.763 1.00
41_V 104_L 1.736 1.00
218_F 271_G 1.714 1.00
44_A 54_T 1.712 1.00
253_Q 274_A 1.706 1.00
113_K 117_E 1.682 1.00
212_Q 215_E 1.681 1.00
201_A 294_W 1.64 1.00
36_V 103_L 1.618 1.00
68_R 91_E 1.607 1.00
40_S 70_L 1.606 1.00
254_M 258_L 1.599 1.00
269_Y 273_C 1.588 1.00
201_A 292_V 1.561 1.00
14_F 112_F 1.558 1.00
256_S 262_I 1.545 1.00
211_H 277_Q 1.52 1.00
35_P 100_R 1.52 1.00
146_H 150_Q 1.481 0.99
234_F 259_E 1.474 0.99
181_T 184_E 1.472 0.99
148_Q 176_F 1.458 0.99
37_E 95_I 1.455 0.99
13_I 101_I 1.447 0.99
207_R 283_I 1.445 0.99
220_Q 224_S 1.445 0.99
135_Q 139_E 1.424 0.99
275_T 278_L 1.415 0.99
28_I 64_A 1.397 0.99
257_Y 264_Y 1.392 0.99
11_K 196_Q 1.388 0.99
280_K 284_T 1.387 0.99
32_K 63_L 1.384 0.99
49_G 236_R 1.374 0.99
44_A 248_C 1.369 0.99
59_A 62_L 1.366 0.99
16_M 112_F 1.359 0.99
27_A 105_V 1.342 0.98
18_P 21_S 1.338 0.98
21_S 204_P 1.337 0.98
28_I 32_K 1.334 0.98
16_M 201_A 1.331 0.98
140_Q 144_S 1.317 0.98
231_R 235_A 1.315 0.98
222_L 264_Y 1.311 0.98
136_Q 176_F 1.308 0.98
35_P 101_I 1.299 0.98
222_L 227_E 1.296 0.98
73_R 83_D 1.296 0.98
77_Q 83_D 1.295 0.98
25_A 61_E 1.294 0.98
91_E 94_D 1.293 0.98
211_H 276_R 1.292 0.98
91_E 95_I 1.289 0.98
211_H 273_C 1.276 0.98
266_E 270_R 1.274 0.98
53_G 251_Y 1.272 0.98
130_R 173_E 1.257 0.97
207_R 211_H 1.256 0.97
209_L 212_Q 1.256 0.97
140_Q 148_Q 1.255 0.97
72_I 87_D 1.252 0.97
155_V 158_A 1.222 0.97
113_K 289_W 1.221 0.97
204_P 209_L 1.213 0.97
92_M 96_T 1.21 0.97
49_G 74_D 1.209 0.97
12_A 92_M 1.205 0.96
29_E 32_K 1.203 0.96
41_V 112_F 1.202 0.96
161_H 164_D 1.199 0.96
83_D 86_R 1.198 0.96
39_I 95_I 1.194 0.96
283_I 287_R 1.186 0.96
132_R 136_Q 1.183 0.96
211_H 272_V 1.181 0.96
36_V 102_P 1.171 0.96
222_L 267_M 1.17 0.96
137_A 165_P 1.168 0.96
147_R 151_E 1.163 0.95
230_V 254_M 1.162 0.95
266_E 269_Y 1.159 0.95
51_D 71_D 1.159 0.95
62_L 71_D 1.154 0.95
199_Q 289_W 1.14 0.95
211_H 215_E 1.132 0.94
38_L 65_A 1.128 0.94
129_V 176_F 1.126 0.94
88_A 115_L 1.117 0.94
218_F 272_V 1.114 0.94
117_E 291_G 1.106 0.93
268_V 272_V 1.097 0.93
215_E 276_R 1.089 0.93
227_E 231_R 1.086 0.93
264_Y 268_V 1.084 0.93
69_L 91_E 1.082 0.93
129_V 133_I 1.081 0.92
90_A 94_D 1.078 0.92
137_A 142_W 1.077 0.92
165_P 168_L 1.074 0.92
159_R 164_D 1.066 0.92
172_L 176_F 1.063 0.92
152_V 172_L 1.061 0.92
141_G 144_S 1.06 0.91
145_L 148_Q 1.057 0.91
15_L 27_A 1.05 0.91
45_L 57_P 1.044 0.91
25_A 220_Q 1.044 0.91
227_E 264_Y 1.041 0.90
28_I 67_H 1.036 0.90
281_R 284_T 1.033 0.90
152_V 176_F 1.033 0.90
216_Q 220_Q 1.031 0.90
200_F 295_L 1.03 0.90
230_V 258_L 1.025 0.90
109_M 286_L 1.022 0.89
34_L 101_I 1.02 0.89
142_W 165_P 1.014 0.89
55_A 221_M 1.01 0.89
131_A 135_Q 1.006 0.88
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3fozA211000.177Contact Map0.817
3exaA40.96841000.215Contact Map0.787
3d3qA20.9811000.222Contact Map0.734
3ephA20.96841000.239Contact Map0.732
3crmA10.9811000.295Contact Map0.752
3a8tA10.81651000.423Contact Map0.702
2ze6A10.7121000.594Contact Map0.577
4e22A10.727899.80.812Contact Map0.275
1ckeA10.705799.80.813Contact Map0.27
2qmhA120.544399.60.841Contact Map0.202
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.0323 seconds.