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OPENSEQ.org
PUR9 - Bifunctional purine biosynthesis protein PurH
UniProt: P15639 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10795
Length: 529 (520)
Sequences: 1418
Seq/Len: 2.73
PUR9
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
406_K 513_D 6.308 1.00
506_D 510_A 5.726 1.00
407_R 413_E 4.617 1.00
325_E 347_S 4.033 1.00
28_S 50_P 3.953 1.00
26_A 157_I 3.687 1.00
482_R 486_D 3.651 1.00
325_E 349_S 3.628 1.00
152_D 178_K 3.593 1.00
12_L 34_L 3.567 1.00
222_K 225_D 3.532 1.00
328_A 355_I 3.374 1.00
9_R 33_E 3.358 1.00
34_L 49_L 3.349 1.00
159_E 171_T 3.151 1.00
333_A 337_R 3.037 1.00
348_A 364_V 3.002 1.00
81_G 90_M 3 1.00
253_T 366_T 2.991 1.00
134_V 179_A 2.989 1.00
118_S 121_D 2.975 1.00
79_I 101_V 2.94 1.00
22_E 25_Q 2.681 1.00
347_S 366_T 2.655 1.00
328_A 351_E 2.615 1.00
485_I 496_V 2.593 1.00
30_R 161_D 2.592 1.00
282_A 296_I 2.571 1.00
145_A 167_L 2.538 1.00
486_D 514_E 2.508 1.00
82_R 85_Q 2.406 1.00
42_R 46_E 2.397 1.00
134_V 146_I 2.354 1.00
498_Q 519_M 2.347 1.00
149_K 178_K 2.332 1.00
328_A 332_Q 2.324 1.00
445_A 448_M 2.249 1.00
224_L 259_A 2.244 1.00
54_V 75_V 2.236 1.00
294_V 423_V 2.184 1.00
353_L 357_A 2.183 1.00
327_D 330_T 2.175 1.00
265_I 382_V 2.158 1.00
472_S 495_C 2.126 1.00
350_E 354_K 2.094 1.00
298_N 302_D 2.076 1.00
283_C 322_F 2.054 1.00
510_A 514_E 2.01 1.00
482_R 507_E 1.979 0.99
510_A 513_D 1.972 0.99
410_S 413_E 1.956 0.99
43_L 47_K 1.951 0.99
159_E 168_T 1.949 0.99
394_G 524_M 1.926 0.99
12_L 24_A 1.921 0.99
23_F 156_I 1.888 0.99
455_K 459_I 1.872 0.99
299_S 302_D 1.868 0.99
57_Y 75_V 1.861 0.99
63_M 93_H 1.846 0.99
334_I 340_V 1.834 0.99
7_V 32_V 1.832 0.99
413_E 494_T 1.804 0.99
301_L 305_D 1.794 0.99
449_S 460_K 1.793 0.99
154_D 158_K 1.776 0.99
109_A 189_M 1.769 0.99
454_A 484_G 1.769 0.99
64_M 90_M 1.76 0.98
119_L 141_H 1.756 0.98
434_Y 441_I 1.722 0.98
45_A 53_E 1.72 0.98
416_D 436_K 1.701 0.98
83_R 99_D 1.697 0.98
482_R 514_E 1.692 0.98
33_E 52_T 1.676 0.98
147_V 156_I 1.659 0.98
473_M 488_A 1.659 0.98
35_L 57_Y 1.657 0.98
41_A 45_A 1.655 0.97
416_D 435_A 1.654 0.97
101_V 132_T 1.635 0.97
420_C 435_A 1.634 0.97
63_M 136_S 1.634 0.97
77_G 95_I 1.608 0.97
26_A 161_D 1.597 0.97
25_Q 29_A 1.593 0.97
146_I 179_A 1.586 0.97
285_I 315_A 1.584 0.96
220_F 386_L 1.582 0.96
281_P 300_I 1.568 0.96
297_G 306_R 1.565 0.96
134_V 148_V 1.564 0.96
266_A 289_A 1.562 0.96
41_A 51_V 1.558 0.96
266_A 380_K 1.557 0.96
434_Y 458_G 1.553 0.96
277_E 426_F 1.544 0.96
21_V 43_L 1.535 0.96
33_E 96_Q 1.53 0.95
297_G 302_D 1.529 0.95
473_M 493_V 1.525 0.95
452_Y 460_K 1.516 0.95
29_A 161_D 1.512 0.95
82_R 88_A 1.503 0.95
158_K 162_D 1.495 0.95
469_K 494_T 1.487 0.94
305_D 333_A 1.485 0.94
504_R 507_E 1.476 0.94
407_R 494_T 1.471 0.94
112_V 122_A 1.47 0.94
118_S 122_A 1.467 0.94
119_L 194_F 1.463 0.94
132_T 137_A 1.447 0.93
260_L 265_I 1.443 0.93
177_I 181_E 1.44 0.93
149_K 152_D 1.439 0.93
119_L 173_F 1.435 0.93
30_R 160_M 1.432 0.93
23_F 147_V 1.426 0.93
417_A 495_C 1.418 0.92
65_D 88_A 1.404 0.92
262_Y 378_D 1.403 0.92
353_L 364_V 1.4 0.92
223_K 240_I 1.397 0.92
155_A 174_D 1.391 0.92
471_S 493_V 1.387 0.91
277_E 425_K 1.368 0.91
486_D 490_A 1.367 0.91
379_F 386_L 1.359 0.90
145_A 175_L 1.356 0.90
411_E 415_R 1.339 0.89
117_C 122_A 1.321 0.88
300_I 322_F 1.318 0.88
496_V 517_I 1.313 0.88
401_L 497_I 1.31 0.88
148_V 182_H 1.302 0.87
256_Q 362_V 1.301 0.87
15_S 125_N 1.293 0.87
432_I 476_D 1.293 0.87
405_T 519_M 1.288 0.87
83_R 143_D 1.281 0.86
177_I 194_F 1.279 0.86
35_L 54_V 1.277 0.86
332_Q 358_A 1.262 0.85
401_L 418_L 1.255 0.85
57_Y 74_K 1.254 0.85
235_Q 390_D 1.253 0.85
109_A 182_H 1.25 0.84
212_F 235_Q 1.243 0.84
77_G 93_H 1.239 0.84
12_L 49_L 1.239 0.84
23_F 102_V 1.235 0.83
503_I 506_D 1.233 0.83
412_Q 437_N 1.229 0.83
290_N 445_A 1.229 0.83
416_D 437_N 1.222 0.83
156_I 171_T 1.22 0.82
41_A 53_E 1.213 0.82
486_D 515_H 1.209 0.82
482_R 511_A 1.203 0.81
119_L 122_A 1.202 0.81
316_F 503_I 1.199 0.81
485_I 508_V 1.197 0.81
320_I 340_V 1.197 0.81
407_R 516_G 1.188 0.80
7_V 100_M 1.186 0.80
498_Q 521_F 1.186 0.80
402_R 523_D 1.182 0.80
65_D 85_Q 1.178 0.79
416_D 438_N 1.168 0.78
511_A 514_E 1.162 0.78
84_G 142_K 1.162 0.78
398_A 414_L 1.161 0.78
101_V 146_I 1.157 0.78
326_L 348_A 1.156 0.77
415_R 438_N 1.156 0.77
26_A 29_A 1.153 0.77
322_F 326_L 1.15 0.77
320_I 334_I 1.146 0.77
487_A 490_A 1.145 0.77
159_E 167_L 1.139 0.76
253_T 347_S 1.136 0.76
402_R 506_D 1.134 0.76
22_E 154_D 1.134 0.76
482_R 515_H 1.132 0.75
489_A 517_I 1.128 0.75
35_L 98_I 1.128 0.75
356_T 364_V 1.127 0.75
132_T 136_S 1.127 0.75
188_S 219_N 1.124 0.75
495_C 518_A 1.124 0.75
274_C 284_V 1.119 0.74
268_T 342_V 1.118 0.74
114_R 121_D 1.116 0.74
52_T 57_Y 1.115 0.74
157_I 161_D 1.112 0.74
15_S 107_P 1.111 0.73
213_P 237_A 1.096 0.72
409_P 413_E 1.091 0.72
458_G 491_A 1.085 0.71
487_A 491_A 1.084 0.71
328_A 349_S 1.083 0.71
272_L 367_C 1.082 0.71
171_T 175_L 1.082 0.71
111_T 117_C 1.079 0.70
413_E 518_A 1.076 0.70
79_I 134_V 1.076 0.70
434_Y 493_V 1.075 0.70
181_E 195_G 1.073 0.70
26_A 30_R 1.068 0.69
403_V 408_Q 1.066 0.69
256_Q 357_A 1.054 0.68
32_V 100_M 1.052 0.68
74_K 94_Q 1.049 0.67
81_G 97_P 1.048 0.67
158_K 161_D 1.045 0.67
329_E 349_S 1.04 0.66
486_D 511_A 1.04 0.66
252_A 365_L 1.037 0.66
268_T 365_L 1.034 0.66
168_T 171_T 1.034 0.66
329_E 332_Q 1.033 0.65
300_I 327_D 1.031 0.65
155_A 171_T 1.03 0.65
347_S 368_G 1.028 0.65
309_K 439_M 1.027 0.65
238_F 382_V 1.027 0.65
412_Q 416_D 1.024 0.64
60_F 93_H 1.018 0.64
445_A 456_I 1.017 0.64
133_M 136_S 1.016 0.64
448_M 456_I 1.013 0.63
212_F 237_A 1.007 0.63
334_I 343_I 1.004 0.62
335_I 356_T 1.002 0.62
403_V 409_P 1.002 0.62
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3zzmA20.97161000.084Contact Map0.677
4ehiA20.9661000.093Contact Map0.708
1g8mA20.94331000.129Contact Map0.679
1zczA20.86391000.14Contact Map0.592
4ggiA10.41781000.847Contact Map0.449
1a9xA40.255299.30.94Contact Map0.579
2yvqA10.234499.30.942Contact Map0.66
2xw6A30.243998.90.951Contact Map0.461
1b93A30.268498.50.956Contact Map0.453
1vmdA20.287398.40.958Contact Map0.503
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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