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OPENSEQ.org
PROW - Glycine betaine/L-proline transport system permease protein ProW
UniProt: P14176 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10772
Length: 354 (282)
Sequences: 713
Seq/Len: 2.53
PROW
Paralog alert: 0.15 [within 20: 0.03] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
241_S 255_V 3.944 1.00
249_R 253_F 3.564 1.00
114_A 130_L 3.35 1.00
169_W 176_A 2.995 1.00
132_S 315_I 2.961 1.00
242_R 251_M 2.951 1.00
75_V 83_F 2.879 1.00
174_P 231_N 2.609 1.00
166_L 223_I 2.593 1.00
172_R 256_Q 2.522 1.00
241_S 251_M 2.424 1.00
160_I 268_V 2.417 1.00
82_V 86_V 2.338 1.00
227_I 231_N 2.267 1.00
115_L 119_Q 1.997 0.99
129_T 319_I 1.954 0.99
176_A 180_I 1.951 0.99
293_M 309_G 1.907 0.99
104_M 109_A 1.878 0.99
63_P 67_W 1.869 0.99
133_L 319_I 1.842 0.99
283_S 295_L 1.786 0.98
177_A 227_I 1.741 0.98
113_F 129_T 1.713 0.98
159_C 190_T 1.688 0.97
158_F 215_I 1.679 0.97
295_L 298_I 1.526 0.95
175_R 179_I 1.518 0.95
98_Q 207_N 1.507 0.94
174_P 227_I 1.504 0.94
110_I 137_G 1.488 0.94
83_F 209_P 1.482 0.94
284_M 295_L 1.454 0.93
170_L 176_A 1.452 0.93
65_D 308_V 1.441 0.92
156_L 298_I 1.435 0.92
323_L 326_A 1.421 0.92
114_A 126_G 1.403 0.91
166_L 180_I 1.399 0.91
109_A 146_M 1.399 0.91
199_I 202_L 1.391 0.90
158_F 161_V 1.388 0.90
197_V 306_A 1.372 0.90
302_D 305_L 1.369 0.89
163_G 283_S 1.351 0.89
265_M 273_M 1.337 0.88
187_M 220_P 1.333 0.88
334_R 337_R 1.326 0.87
295_L 309_G 1.32 0.87
109_A 112_V 1.315 0.87
117_A 129_T 1.306 0.86
100_L 104_M 1.302 0.86
144_Q 293_M 1.293 0.86
149_L 316_L 1.282 0.85
96_G 100_L 1.279 0.85
123_V 127_V 1.266 0.84
118_W 126_G 1.264 0.84
170_L 227_I 1.261 0.84
187_M 216_I 1.261 0.84
102_L 146_M 1.257 0.83
100_L 103_G 1.252 0.83
75_V 78_H 1.247 0.83
251_M 263_T 1.242 0.82
163_G 250_Q 1.22 0.81
199_I 307_T 1.219 0.81
297_G 309_G 1.21 0.80
117_A 323_L 1.207 0.80
220_P 275_A 1.206 0.80
230_I 256_Q 1.201 0.79
157_L 161_V 1.201 0.79
121_S 124_G 1.19 0.78
125_M 241_S 1.188 0.78
95_N 143_S 1.183 0.78
292_Q 299_G 1.183 0.78
285_I 325_Q 1.172 0.77
159_C 283_S 1.164 0.76
230_I 238_I 1.163 0.76
61_L 138_A 1.161 0.76
64_L 135_A 1.156 0.75
87_R 204_G 1.156 0.75
141_A 312_G 1.148 0.75
293_M 296_R 1.141 0.74
109_A 131_V 1.138 0.74
193_F 250_Q 1.135 0.74
166_L 219_L 1.135 0.74
295_L 299_G 1.132 0.73
146_M 149_L 1.125 0.73
299_G 302_D 1.123 0.72
111_I 178_K 1.119 0.72
80_R 306_A 1.117 0.72
276_L 294_V 1.111 0.71
222_I 271_T 1.109 0.71
211_V 285_I 1.108 0.71
202_L 307_T 1.106 0.71
174_P 178_K 1.104 0.71
284_M 298_I 1.1 0.70
234_P 259_L 1.097 0.70
75_V 79_F 1.091 0.69
164_L 240_A 1.089 0.69
251_M 255_V 1.082 0.68
72_I 213_V 1.08 0.68
234_P 237_L 1.071 0.67
141_A 210_G 1.07 0.67
221_P 269_N 1.062 0.66
219_L 223_I 1.06 0.66
177_A 181_R 1.052 0.65
269_N 324_T 1.038 0.64
171_A 209_P 1.032 0.63
181_R 288_G 1.031 0.63
263_T 275_A 1.027 0.62
276_L 314_V 1.023 0.62
244_F 266_A 1.018 0.61
65_D 69_T 1.018 0.61
116_I 175_R 1.014 0.61
217_F 277_S 1.013 0.61
185_D 224_R 1.01 0.61
141_A 285_I 1.007 0.60
111_I 169_W 1.002 0.60
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3tuiA40.570699.90.817Contact Map0.589
2onkC40.754299.90.823Contact Map0.512
3d31C20.776899.90.824Contact Map0.468
3rlfF10.895599.90.837Contact Map0.369
3rlfG10.737399.80.849Contact Map0.405
3arcK20.104524.60.976Contact Map0.065
4ixqK20.129915.80.978Contact Map0.056
4humA10.30232.30.985Contact Map0.055
3h7aA40.19771.80.986Contact Map0.245
4f35D40.69771.80.986Contact Map0.156
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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