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OPENSEQ.org
NHAA - Na(+)/H(+) antiporter NhaA
UniProt: P13738 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10652
Length: 388 (382)
Sequences: 925
Seq/Len: 2.42
NHAA
Paralog alert: 0.11 [within 20: 0.03] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
105_M 166_G 4.736 1.00
112_Y 350_F 4.072 1.00
58_N 61_L 3.385 1.00
116_N 291_G 3.356 1.00
301_P 329_M 3.294 1.00
346_A 361_A 3.007 1.00
130_A 170_I 2.89 1.00
191_I 232_I 2.889 1.00
113_L 123_R 2.762 1.00
298_I 302_L 2.656 1.00
297_L 375_I 2.594 1.00
304_I 335_C 2.391 1.00
99_I 311_A 2.279 1.00
186_V 218_A 2.257 1.00
190_A 215_L 2.147 1.00
361_A 365_I 2.072 1.00
305_S 329_M 1.992 0.99
333_I 379_W 1.97 0.99
181_M 185_G 1.945 0.99
180_S 183_S 1.924 0.99
151_A 323_T 1.917 0.99
90_S 158_A 1.913 0.99
81_R 252_E 1.912 0.99
330_V 379_W 1.882 0.99
28_M 269_F 1.878 0.99
28_M 37_W 1.877 0.99
16_I 20_I 1.866 0.99
42_L 270_A 1.83 0.99
63_I 270_A 1.829 0.99
105_M 170_I 1.814 0.98
333_I 375_I 1.795 0.98
187_A 219_V 1.778 0.98
64_N 271_N 1.77 0.98
309_W 313_R 1.769 0.98
139_G 143_L 1.756 0.98
14_G 260_A 1.743 0.98
193_V 211_V 1.742 0.98
312_L 328_I 1.723 0.98
197_L 207_V 1.697 0.97
24_L 269_F 1.688 0.97
183_S 218_A 1.687 0.97
368_G 372_S 1.676 0.97
111_L 294_A 1.653 0.97
191_I 215_L 1.627 0.96
169_I 173_L 1.607 0.96
197_L 211_V 1.588 0.96
228_L 232_I 1.586 0.96
31_S 34_T 1.584 0.96
297_L 372_S 1.579 0.95
158_A 162_I 1.578 0.95
293_I 371_S 1.556 0.95
138_L 157_M 1.552 0.95
127_A 130_A 1.532 0.94
38_Y 273_G 1.529 0.94
190_A 211_V 1.525 0.94
41_F 59_M 1.519 0.94
194_L 233_V 1.514 0.94
42_L 60_L 1.508 0.94
77_L 234_G 1.508 0.94
195_A 236_F 1.473 0.93
306_L 310_L 1.47 0.93
234_G 330_V 1.46 0.92
45_P 56_N 1.459 0.92
47_Q 56_N 1.443 0.92
262_L 266_L 1.443 0.92
191_I 195_A 1.431 0.91
111_L 298_I 1.431 0.91
25_A 272_A 1.428 0.91
167_A 341_M 1.403 0.90
301_P 305_S 1.399 0.90
212_G 233_V 1.396 0.90
297_L 371_S 1.392 0.90
297_L 301_P 1.386 0.89
297_L 329_M 1.378 0.89
129_P 134_I 1.36 0.88
65_D 225_H 1.357 0.88
126_W 161_I 1.345 0.88
112_Y 288_L 1.34 0.87
131_A 134_I 1.326 0.87
13_S 261_Y 1.323 0.86
57_K 61_L 1.321 0.86
96_F 312_L 1.316 0.86
39_H 43_E 1.309 0.86
187_A 218_A 1.309 0.86
77_L 251_L 1.302 0.85
157_M 234_G 1.299 0.85
26_M 366_L 1.297 0.85
73_L 205_T 1.295 0.85
286_S 360_W 1.29 0.84
210_L 214_V 1.287 0.84
305_S 332_G 1.279 0.84
289_P 364_G 1.276 0.84
103_G 320_P 1.274 0.83
110_L 113_L 1.271 0.83
114_A 173_L 1.271 0.83
190_A 218_A 1.263 0.83
185_G 189_V 1.249 0.82
342_S 345_I 1.242 0.81
127_A 170_I 1.242 0.81
293_I 297_L 1.239 0.81
134_I 171_I 1.239 0.81
188_A 274_V 1.236 0.81
196_V 200_C 1.231 0.80
103_G 307_F 1.226 0.80
140_V 337_I 1.222 0.80
319_L 323_T 1.221 0.80
99_I 307_F 1.219 0.79
57_K 221_K 1.217 0.79
301_P 306_L 1.216 0.79
75_V 138_L 1.215 0.79
346_A 365_I 1.203 0.78
95_A 99_I 1.193 0.77
293_I 368_G 1.191 0.77
294_A 298_I 1.19 0.77
35_S 40_D 1.184 0.77
115_F 294_A 1.182 0.77
198_N 237_I 1.179 0.76
40_D 43_E 1.176 0.76
256_H 260_A 1.173 0.76
94_A 268_A 1.172 0.76
168_I 364_G 1.17 0.75
134_I 165_L 1.166 0.75
7_F 256_H 1.164 0.75
190_A 214_V 1.163 0.75
194_L 215_L 1.153 0.74
7_F 13_S 1.144 0.73
73_L 233_V 1.139 0.73
269_F 332_G 1.138 0.73
80_K 84_M 1.135 0.72
114_A 117_Y 1.132 0.72
217_T 221_K 1.123 0.71
301_P 332_G 1.118 0.71
73_L 212_G 1.111 0.70
307_F 311_A 1.11 0.70
304_I 337_I 1.108 0.70
89_A 158_A 1.108 0.70
364_G 368_G 1.106 0.70
194_L 212_G 1.103 0.69
62_W 66_A 1.1 0.69
377_Y 381_R 1.095 0.68
186_V 190_A 1.089 0.68
148_V 153_K 1.085 0.67
68_M 271_N 1.069 0.66
213_V 217_T 1.069 0.66
63_I 263_I 1.069 0.66
185_G 188_A 1.069 0.66
109_A 161_I 1.063 0.65
366_L 370_I 1.059 0.65
22_A 26_M 1.058 0.65
127_A 368_G 1.058 0.65
258_W 262_L 1.054 0.64
323_T 331_V 1.053 0.64
103_G 235_F 1.052 0.64
26_M 269_F 1.049 0.64
107_V 302_L 1.046 0.63
301_P 343_I 1.04 0.63
43_E 60_L 1.039 0.62
130_A 166_G 1.035 0.62
137_A 331_V 1.035 0.62
111_L 291_G 1.025 0.61
64_N 68_M 1.025 0.61
101_A 369_S 1.023 0.61
45_P 61_L 1.022 0.61
125_G 350_F 1.02 0.60
127_A 331_V 1.018 0.60
194_L 198_N 1.017 0.60
289_P 360_W 1.015 0.60
34_T 39_H 1.011 0.59
232_I 236_F 1.011 0.59
3_H 73_L 1.007 0.59
62_W 226_A 1.006 0.59
373_A 377_Y 1.005 0.59
79_V 344_F 1.004 0.59
8_F 13_S 1.003 0.58
312_L 325_Y 1 0.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1zcdA20.99741000.282Contact Map0.429
4bwzA10.899599.80.867Contact Map0.405
3zuxA10.762921.30.979Contact Map0.247
1q90G10.09544.60.984Contact Map0.65
3orgA40.44333.90.985Contact Map0.095
2kluA10.09023.50.985Contact Map0.269
4h44G10.09542.70.986Contact Map0.508
3giaA10.17272.70.986Contact Map0.335
3bz1X10.09542.50.986Contact Map0.155
2ifoA10.11862.40.987Contact Map0.332
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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