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OPENSEQ.org
TRMH - tRNA (guanosine(18)-2'-O)-methyltransferase
UniProt: P0AGJ2 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10967
Length: 229 (228)
Sequences: 324
Seq/Len: 1.42
TRMH
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
36_I 150_V 2.77 1.00
210_N 214_E 2.628 1.00
47_E 74_K 2.58 1.00
92_Q 112_R 2.558 1.00
49_H 76_H 2.416 1.00
92_Q 114_T 2.275 0.99
210_N 216_E 2.13 0.99
8_R 11_E 2.004 0.98
127_Q 131_A 1.984 0.98
64_A 68_N 1.933 0.97
224_T 227_A 1.65 0.91
3_P 7_A 1.648 0.91
95_A 125_I 1.62 0.90
87_K 93_I 1.614 0.90
219_A 223_A 1.591 0.89
206_Y 209_V 1.579 0.89
80_G 128_E 1.577 0.89
94_L 135_Q 1.535 0.87
98_L 146_Q 1.528 0.86
25_E 129_A 1.467 0.83
184_Q 209_V 1.436 0.81
78_T 81_D 1.435 0.81
7_A 11_E 1.434 0.81
23_C 82_A 1.419 0.80
42_A 169_G 1.398 0.79
65_A 68_N 1.397 0.79
21_T 49_H 1.395 0.79
197_K 201_R 1.384 0.78
42_A 145_V 1.376 0.77
22_V 40_A 1.336 0.74
14_A 183_Q 1.328 0.74
29_K 120_Q 1.326 0.74
195_L 198_V 1.292 0.71
111_T 161_A 1.288 0.71
160_E 163_R 1.265 0.69
41_D 145_V 1.264 0.69
95_A 117_L 1.261 0.68
91_M 94_L 1.254 0.68
17_Q 171_Y 1.232 0.66
10_C 187_L 1.231 0.66
46_H 72_Q 1.226 0.65
118_M 150_V 1.216 0.64
21_T 76_H 1.194 0.62
208_H 212_Q 1.192 0.62
106_R 161_A 1.191 0.62
128_E 139_I 1.186 0.61
146_Q 153_A 1.186 0.61
208_H 216_E 1.185 0.61
195_L 199_A 1.184 0.61
117_L 129_A 1.183 0.61
120_Q 190_G 1.178 0.61
20_L 160_E 1.173 0.60
48_V 71_V 1.17 0.60
211_Q 220_D 1.169 0.60
68_N 115_C 1.165 0.59
224_T 228_A 1.155 0.58
62_S 115_C 1.148 0.58
64_A 67_S 1.148 0.58
22_V 158_L 1.131 0.56
217_A 222_W 1.119 0.55
19_D 111_T 1.119 0.55
85_H 88_G 1.118 0.55
31_H 190_G 1.114 0.54
144_M 150_V 1.113 0.54
22_V 54_G 1.109 0.54
220_D 223_A 1.108 0.54
18_P 175_N 1.106 0.53
144_M 164_Q 1.105 0.53
17_Q 164_Q 1.1 0.53
212_Q 216_E 1.09 0.52
113_P 129_A 1.088 0.52
83_V 126_T 1.081 0.51
194_V 199_A 1.077 0.50
84_A 93_I 1.077 0.50
34_S 41_D 1.074 0.50
102_A 153_A 1.074 0.50
92_Q 113_P 1.073 0.50
25_E 126_T 1.066 0.49
195_L 226_Q 1.061 0.49
23_C 83_V 1.057 0.48
48_V 63_A 1.048 0.47
42_A 114_T 1.045 0.47
138_I 146_Q 1.045 0.47
144_M 169_G 1.044 0.47
189_E 193_P 1.039 0.47
110_Y 164_Q 1.038 0.46
118_M 125_I 1.036 0.46
29_K 188_F 1.034 0.46
5_R 8_R 1.029 0.45
221_W 225_M 1.026 0.45
194_V 198_V 1.026 0.45
25_E 82_A 1.026 0.45
23_C 115_C 1.025 0.45
137_I 178_L 1.021 0.45
203_G 219_A 1.021 0.45
19_D 109_D 1.021 0.45
20_L 114_T 1.019 0.44
41_D 106_R 1.019 0.44
41_D 73_V 1.019 0.44
11_E 180_E 1.018 0.44
116_I 137_I 1.018 0.44
110_Y 161_A 1.017 0.44
84_A 88_G 1.016 0.44
109_D 114_T 1.015 0.44
67_S 145_V 1.014 0.44
108_I 156_L 1.01 0.44
101_N 127_Q 1.009 0.43
5_R 42_A 1.006 0.43
200_K 212_Q 1.001 0.43
27_V 148_L 1 0.43
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1v2xA10.83411000.482Contact Map0.462
1zjrA10.89081000.485Contact Map0.515
1gz0A80.72051000.594Contact Map0.549
2ha8A20.7381000.594Contact Map0.4
1ipaA10.76421000.595Contact Map0.438
1x7oA20.74671000.603Contact Map0.388
3nk6A20.74671000.608Contact Map0.391
4kgnA80.67691000.609Contact Map0.349
3n4jA10.68561000.615Contact Map0.391
2i6dA10.71181000.624Contact Map0.486
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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