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OPENSEQ.org
SECF - Protein translocase subunit SecF
UniProt: P0AG93 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10940
Length: 323 (302)
Sequences: 1750
Seq/Len: 5.79
SECF
Paralog alert: 0.08 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
18_D 238_N 4.991 1.00
40_I 189_S 4.603 1.00
41_M 46_F 3.845 1.00
188_L 276_T 3.471 1.00
224_N 243_Q 3.318 1.00
18_D 21_R 3.26 1.00
256_L 278_L 3.229 1.00
224_N 240_S 3.116 1.00
155_L 159_V 2.994 1.00
27_F 298_K 2.859 1.00
67_E 70_V 2.816 1.00
263_Y 278_L 2.406 1.00
263_Y 271_E 2.392 1.00
187_I 279_I 2.29 1.00
25_W 29_I 2.2 1.00
49_G 196_D 2.17 1.00
39_V 43_V 2.135 1.00
151_M 155_L 2.073 1.00
252_S 285_T 2.058 1.00
59_E 130_R 2.019 1.00
147_A 151_M 1.992 1.00
126_A 129_K 1.961 1.00
260_L 264_L 1.945 1.00
241_L 289_I 1.941 1.00
221_I 293_S 1.926 1.00
162_G 170_A 1.874 1.00
22_W 25_W 1.865 1.00
33_L 37_A 1.838 1.00
37_A 182_I 1.834 1.00
30_S 286_A 1.827 1.00
40_I 190_L 1.822 1.00
263_Y 275_L 1.82 1.00
38_I 42_G 1.791 1.00
180_D 210_S 1.77 1.00
179_H 286_A 1.767 1.00
37_A 186_G 1.736 1.00
240_S 243_Q 1.686 1.00
249_L 253_G 1.684 1.00
30_S 179_H 1.682 1.00
187_I 191_F 1.677 1.00
237_F 293_S 1.674 1.00
157_I 211_L 1.641 1.00
232_T 235_E 1.635 1.00
172_G 218_S 1.632 1.00
259_I 281_V 1.613 0.99
225_F 233_P 1.597 0.99
259_I 278_L 1.588 0.99
176_A 180_D 1.584 0.99
136_P 139_G 1.558 0.99
21_R 238_N 1.545 0.99
57_V 130_R 1.54 0.99
239_V 243_Q 1.523 0.99
15_K 242_T 1.513 0.99
68_I 72_R 1.513 0.99
22_W 26_A 1.512 0.99
179_H 214_S 1.511 0.99
144_Q 148_M 1.5 0.99
160_Y 164_R 1.49 0.99
34_L 182_I 1.483 0.99
176_A 292_A 1.477 0.99
161_V 215_I 1.449 0.99
70_V 73_D 1.428 0.98
116_K 120_E 1.426 0.98
112_S 116_K 1.416 0.98
252_S 255_T 1.409 0.98
218_S 292_A 1.391 0.98
181_V 207_I 1.386 0.98
23_D 298_K 1.373 0.98
172_G 295_L 1.368 0.98
24_Y 36_A 1.359 0.98
23_D 27_F 1.348 0.97
235_E 239_V 1.34 0.97
195_I 199_I 1.331 0.97
34_L 37_A 1.321 0.97
46_F 189_S 1.32 0.97
236_I 240_S 1.313 0.97
295_L 299_L 1.31 0.97
270_L 274_S 1.3 0.97
187_I 275_L 1.264 0.96
256_L 282_S 1.254 0.96
184_T 206_V 1.253 0.96
73_D 76_Q 1.252 0.95
176_A 179_H 1.249 0.95
180_D 287_S 1.247 0.95
158_L 174_V 1.237 0.95
213_D 292_A 1.225 0.95
41_M 185_L 1.219 0.95
107_G 112_S 1.2 0.94
25_W 28_G 1.184 0.93
74_A 121_S 1.182 0.93
51_D 134_V 1.172 0.93
48_W 143_A 1.162 0.93
145_T 288_S 1.162 0.93
250_I 253_G 1.152 0.92
196_D 272_G 1.15 0.92
27_F 31_G 1.147 0.92
198_T 201_A 1.141 0.92
33_L 186_G 1.13 0.91
167_W 171_A 1.129 0.91
237_F 297_L 1.128 0.91
72_R 216_V 1.117 0.90
285_T 288_S 1.113 0.90
255_T 281_V 1.113 0.90
168_R 305_H 1.108 0.90
16_V 242_T 1.104 0.90
248_T 285_T 1.103 0.90
228_I 231_G 1.097 0.89
56_T 131_I 1.086 0.89
85_L 89_G 1.084 0.89
68_I 85_L 1.083 0.89
175_I 295_L 1.082 0.89
268_P 271_E 1.079 0.88
44_R 192_H 1.074 0.88
156_S 161_V 1.073 0.88
46_F 195_I 1.058 0.87
32_L 39_V 1.053 0.87
211_L 215_I 1.041 0.86
115_L 119_N 1.039 0.86
88_F 130_R 1.038 0.86
148_M 152_A 1.038 0.86
166_E 300_G 1.037 0.86
179_H 287_S 1.035 0.85
185_L 203_L 1.031 0.85
44_R 189_S 1.031 0.85
35_I 39_V 1.026 0.85
33_L 183_I 1.025 0.85
18_D 234_Y 1.021 0.84
20_M 238_N 1.019 0.84
110_L 113_Q 1.018 0.84
58_I 131_I 1.018 0.84
10_L 13_G 1.016 0.84
73_D 77_K 1.01 0.83
157_I 161_V 1.008 0.83
228_I 239_V 1.003 0.83
63_E 124_Q 1.002 0.83
256_L 260_L 1.002 0.83
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3aqpA20.95671000.536Contact Map0.591
3w9iA60.953699.80.802Contact Map0.683
3ne5A10.931999.80.803Contact Map0.519
4dx5A30.96999.70.811Contact Map0.627
4k0jA60.972199.70.822Contact Map0.613
3aqoA40.368460.80.96Contact Map0.217
2l2tA20.136211.60.973Contact Map0.833
2kluA10.1618.10.975Contact Map0.224
2jwaA20.137.10.975Contact Map0.522
2ks1B10.13315.70.976Contact Map0.678
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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