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OPENSEQ.org

RISA - Riboflavin synthase
UniProt: P0AFU8 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11406
Length: 213 (200)
Sequences: 1765
Seq/Len: 8.82

RISA
Paralog alert: 0.04 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
89_K 92_D 3.594 1.00
107_A 123_F 3.145 1.00
70_I 136_Y 2.995 1.00
108_E 124_K 2.807 1.00
88_A 94_I 2.714 1.00
143_D 174_K 2.557 1.00
90_F 98_L 2.555 1.00
134_I 149_V 2.462 1.00
11_K 27_E 2.39 1.00
105_T 130_L 2.385 1.00
145_I 171_L 2.107 1.00
142_I 180_V 2.069 1.00
10_A 84_V 2.037 1.00
36_L 42_V 2.018 1.00
132_K 196_E 2.015 1.00
27_E 57_N 1.95 1.00
109_V 174_K 1.937 1.00
13_V 27_E 1.903 1.00
145_I 170_T 1.875 1.00
81_W 173_K 1.833 1.00
76_L 80_D 1.826 1.00
174_K 178_A 1.826 1.00
7_Q 86_R 1.82 1.00
64_M 160_H 1.79 1.00
122_W 156_R 1.789 1.00
53_E 60_S 1.781 1.00
47_C 71_T 1.773 1.00
25_V 60_S 1.76 1.00
110_A 124_K 1.756 1.00
122_W 158_C 1.753 1.00
11_K 81_W 1.739 1.00
164_E 168_R 1.725 1.00
108_E 179_R 1.67 1.00
191_V 195_V 1.656 1.00
90_F 94_I 1.648 1.00
44_H 59_V 1.639 1.00
75_D 179_R 1.633 1.00
109_V 121_I 1.633 1.00
142_I 182_I 1.562 1.00
145_I 161_L 1.559 1.00
112_I 121_I 1.558 1.00
66_E 137_K 1.543 1.00
44_H 82_V 1.529 1.00
37_E 40_A 1.493 1.00
133_Y 196_E 1.486 1.00
98_L 186_P 1.484 1.00
119_R 166_L 1.46 1.00
7_Q 93_E 1.459 1.00
134_I 157_F 1.451 0.99
135_L 186_P 1.412 0.99
99_M 141_G 1.403 0.99
125_V 182_I 1.388 0.99
65_K 69_R 1.384 0.99
151_E 158_C 1.38 0.99
153_T 156_R 1.378 0.99
143_D 170_T 1.372 0.99
23_T 52_T 1.364 0.99
8_G 28_L 1.356 0.99
118_N 160_H 1.344 0.99
124_K 156_R 1.34 0.99
124_K 155_T 1.325 0.99
86_R 93_E 1.309 0.99
135_L 193_D 1.299 0.99
24_H 63_L 1.296 0.99
87_A 93_E 1.286 0.98
171_L 174_K 1.284 0.98
150_G 158_C 1.284 0.98
125_V 134_I 1.28 0.98
142_I 159_V 1.273 0.98
25_V 58_H 1.27 0.98
94_I 98_L 1.268 0.98
109_V 123_F 1.262 0.98
166_L 172_G 1.225 0.98
17_E 22_R 1.215 0.98
9_T 81_W 1.211 0.98
150_G 160_H 1.204 0.97
112_I 119_R 1.202 0.97
45_N 72_N 1.193 0.97
145_I 174_K 1.191 0.97
123_F 159_V 1.189 0.97
135_L 192_V 1.184 0.97
38_T 164_E 1.173 0.97
107_A 142_I 1.17 0.97
120_Q 158_C 1.161 0.97
69_R 115_S 1.159 0.97
36_L 54_I 1.155 0.96
89_K 94_I 1.141 0.96
55_N 58_H 1.138 0.96
65_K 115_S 1.135 0.96
52_T 62_D 1.129 0.96
45_N 76_L 1.129 0.96
121_I 161_L 1.128 0.96
111_K 122_W 1.12 0.96
109_V 180_V 1.119 0.96
35_G 93_E 1.115 0.95
40_A 95_G 1.114 0.95
45_N 143_D 1.108 0.95
77_K 80_D 1.094 0.95
68_L 73_L 1.092 0.95
134_I 140_I 1.083 0.94
12_L 26_V 1.082 0.94
64_M 150_G 1.078 0.94
35_G 95_G 1.061 0.94
112_I 175_K 1.043 0.93
40_A 93_E 1.033 0.92
111_K 176_L 1.033 0.92
88_A 141_G 1.031 0.92
125_V 157_F 1.027 0.92
115_S 120_Q 1.024 0.92
136_Y 151_E 1.013 0.91
161_L 166_L 1.001 0.91
107_A 182_I 1 0.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1i8dA30.99531000.063Contact Map0.815
1kzlA10.97181000.12Contact Map0.609
4e0fA30.96241000.133Contact Map0.837
3a35A20.8921000.165Contact Map0.695
3ddyA10.87321000.176Contact Map0.722
3h43A120.305226.40.961Contact Map0.626
2wg5A120.309923.30.962Contact Map0.707
2wfwA30.596213.10.966Contact Map0.229
2f9hA20.413111.90.966Contact Map0.481
2qcpX10.267610.60.967Contact Map0.612

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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