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OPENSEQ.org
PROX - Glycine betaine-binding periplasmic protein
UniProt: P0AFM2 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10773
Length: 330 (296)
Sequences: 1022
Seq/Len: 3.45
PROX
Paralog alert: 0.22 [within 20: 0.07] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
121_K 125_D 3.373 1.00
262_N 268_K 3.197 1.00
121_K 223_D 3.124 1.00
91_D 95_E 2.949 1.00
105_R 258_H 2.903 1.00
121_K 225_V 2.902 1.00
123_T 203_P 2.694 1.00
143_L 203_P 2.554 1.00
48_V 275_L 2.445 1.00
195_I 218_L 2.391 1.00
120_D 123_T 2.318 1.00
123_T 144_F 2.294 1.00
34_V 70_G 2.29 1.00
129_I 144_F 2.221 1.00
30_T 58_T 2.203 1.00
33_P 81_F 2.04 1.00
314_H 317_Q 2.018 1.00
157_C 163_C 1.97 1.00
130_T 134_Q 1.952 1.00
50_R 53_E 1.922 1.00
124_A 225_V 1.92 1.00
34_V 79_A 1.92 1.00
309_G 313_A 1.895 1.00
47_L 307_V 1.893 1.00
122_K 201_G 1.883 1.00
144_F 203_P 1.875 1.00
315_Q 319_D 1.869 0.99
48_V 83_A 1.803 0.99
55_L 275_L 1.742 0.99
66_D 69_V 1.74 0.99
117_Y 167_I 1.739 0.99
49_S 53_E 1.727 0.99
282_P 285_D 1.712 0.99
195_I 199_K 1.711 0.99
285_D 317_Q 1.644 0.98
49_S 62_P 1.641 0.98
317_Q 320_G 1.64 0.98
208_T 214_V 1.636 0.98
43_F 293_M 1.625 0.98
122_K 198_Y 1.619 0.98
50_R 308_D 1.61 0.98
54_K 315_Q 1.61 0.98
118_L 208_T 1.609 0.98
31_V 272_A 1.609 0.98
316_Q 319_D 1.595 0.98
129_I 140_I 1.574 0.98
40_E 84_V 1.573 0.98
291_A 295_D 1.559 0.98
194_T 206_Y 1.552 0.97
104_Y 266_A 1.521 0.97
119_I 124_A 1.52 0.97
104_Y 261_A 1.496 0.97
129_I 135_L 1.482 0.96
40_E 286_I 1.48 0.96
117_Y 154_L 1.475 0.96
292_I 309_G 1.47 0.96
32_N 61_K 1.448 0.96
158_N 187_Y 1.447 0.96
265_W 269_N 1.442 0.96
113_A 255_S 1.378 0.94
65_V 70_G 1.37 0.94
130_T 225_V 1.369 0.94
187_Y 191_M 1.368 0.94
116_G 132_I 1.364 0.94
115_Q 163_C 1.358 0.93
43_F 289_Q 1.352 0.93
135_L 144_F 1.348 0.93
95_E 99_G 1.344 0.93
264_A 268_K 1.339 0.93
52_L 272_A 1.334 0.93
106_E 277_A 1.329 0.92
308_D 312_K 1.319 0.92
126_Q 203_P 1.315 0.92
32_N 63_S 1.314 0.92
164_E 184_Q 1.313 0.92
41_E 84_V 1.312 0.92
108_V 258_H 1.31 0.92
274_K 278_I 1.288 0.91
293_M 297_K 1.273 0.90
115_Q 161_W 1.267 0.90
71_Y 85_N 1.251 0.89
193_D 197_R 1.251 0.89
195_I 217_E 1.25 0.89
123_T 143_L 1.241 0.88
169_H 316_Q 1.236 0.88
116_G 215_S 1.234 0.88
210_T 287_N 1.234 0.88
30_T 61_K 1.223 0.87
120_D 201_G 1.215 0.87
122_K 129_I 1.214 0.87
120_D 129_I 1.203 0.86
261_A 273_A 1.197 0.86
104_Y 263_K 1.176 0.85
272_A 276_F 1.173 0.84
168_N 172_A 1.172 0.84
274_K 277_A 1.157 0.83
31_V 269_N 1.152 0.83
123_T 129_I 1.151 0.83
265_W 273_A 1.147 0.83
163_C 207_Y 1.142 0.82
283_V 287_N 1.138 0.82
48_V 279_M 1.135 0.82
141_A 146_T 1.133 0.81
274_K 312_K 1.128 0.81
155_T 193_D 1.124 0.81
125_D 225_V 1.121 0.81
50_R 311_I 1.121 0.81
174_Y 229_V 1.117 0.80
66_D 73_S 1.114 0.80
81_F 276_F 1.114 0.80
117_Y 205_F 1.107 0.79
265_W 272_A 1.105 0.79
58_T 202_K 1.103 0.79
85_N 162_G 1.102 0.79
40_E 44_Q 1.099 0.79
171_L 182_H 1.069 0.76
90_H 162_G 1.067 0.76
111_N 256_T 1.058 0.75
49_S 59_V 1.058 0.75
123_T 126_Q 1.056 0.75
129_I 134_Q 1.055 0.75
275_L 279_M 1.053 0.74
290_N 293_M 1.051 0.74
266_A 314_H 1.042 0.73
75_A 102_K 1.038 0.73
119_I 123_T 1.035 0.73
69_V 185_G 1.014 0.70
109_F 259_I 1.011 0.70
89_L 162_G 1.01 0.70
196_S 200_E 1.008 0.70
172_A 201_G 1.005 0.69
51_A 311_I 1.001 0.69
36_S 70_G 1.001 0.69
135_L 205_F 1.001 0.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1r9lA10.93031000.421Contact Map0.678
4ne4A10.79091000.557Contact Map0.526
2rinA20.83641000.571Contact Map0.723
3o66A20.77581000.575Contact Map0.597
2b4lA10.4971000.596Contact Map0.683
3r6uA10.78791000.618Contact Map0.551
3l6gA10.76971000.62Contact Map0.726
3tmgA40.84241000.629Contact Map0.78
1sw5A40.76361000.646Contact Map0.633
1p99A10.781899.40.847Contact Map0.554
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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