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OPENSEQ.org
NUSA - Transcription elongation protein NusA
UniProt: P0AFF6 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10665
Length: 495 (491)
Sequences: 583
Seq/Len: 1.19
NUSA
Paralog alert: 0.00 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
152_L 197_V 3.025 1.00
181_G 197_V 2.977 1.00
63_L 92_Y 2.845 1.00
432_D 436_N 2.697 1.00
207_E 210_R 2.561 1.00
313_N 316_Q 2.461 0.99
484_A 488_I 2.432 0.99
63_L 74_E 2.388 0.99
162_I 181_G 2.196 0.98
437_L 456_L 2.183 0.98
307_I 336_L 2.144 0.98
84_D 91_D 2.023 0.97
57_D 96_Q 1.992 0.97
248_V 252_V 1.966 0.96
441_D 477_K 1.966 0.96
457_E 489_C 1.96 0.96
299_D 302_K 1.958 0.96
221_I 245_I 1.948 0.96
435_L 445_A 1.943 0.96
456_L 489_C 1.931 0.96
205_L 234_A 1.923 0.96
225_A 283_F 1.917 0.96
160_A 197_V 1.911 0.96
357_D 361_K 1.898 0.95
183_L 195_L 1.892 0.95
130_F 134_E 1.85 0.94
447_K 450_A 1.804 0.93
437_L 484_A 1.787 0.93
474_T 477_K 1.741 0.92
311_A 342_D 1.726 0.91
87_L 91_D 1.714 0.91
213_V 259_V 1.713 0.91
88_N 91_D 1.637 0.88
398_L 403_V 1.63 0.88
203_E 206_I 1.599 0.86
66_D 91_D 1.597 0.86
127_V 186_V 1.586 0.86
240_T 245_I 1.584 0.86
236_I 259_V 1.574 0.85
184_Y 201_K 1.555 0.84
37_K 205_L 1.536 0.83
259_V 273_I 1.536 0.83
144_K 148_D 1.487 0.80
81_R 85_E 1.476 0.80
284_V 305_M 1.467 0.79
224_K 239_K 1.442 0.77
24_F 51_R 1.442 0.77
464_I 479_G 1.438 0.77
66_D 69_T 1.437 0.77
77_L 81_R 1.435 0.77
408_E 412_N 1.425 0.76
480_A 488_I 1.423 0.76
416_T 420_A 1.415 0.76
88_N 453_V 1.409 0.75
415_A 419_Q 1.407 0.75
76_T 79_A 1.403 0.75
466_D 469_D 1.389 0.74
423_E 427_D 1.389 0.74
462_Q 466_D 1.383 0.73
219_E 241_N 1.374 0.72
68_V 74_E 1.374 0.72
20_R 66_D 1.363 0.72
440_V 445_A 1.354 0.71
365_I 369_F 1.353 0.71
443_D 472_G 1.351 0.71
127_V 131_R 1.349 0.70
70_Q 336_L 1.344 0.70
457_E 462_Q 1.342 0.70
252_V 273_I 1.339 0.70
103_D 106_T 1.338 0.70
10_E 20_R 1.328 0.69
440_V 456_L 1.313 0.67
443_D 447_K 1.313 0.67
68_V 76_T 1.31 0.67
198_T 201_K 1.307 0.67
346_A 350_A 1.301 0.66
145_V 312_G 1.301 0.66
151_S 343_D 1.298 0.66
309_V 334_W 1.296 0.66
120_E 124_A 1.293 0.66
12_V 158_A 1.291 0.66
280_P 305_M 1.286 0.65
230_P 234_A 1.285 0.65
450_A 454_C 1.282 0.65
416_T 423_E 1.277 0.64
305_M 332_S 1.275 0.64
135_G 250_A 1.272 0.64
263_S 270_R 1.269 0.64
401_P 405_A 1.269 0.64
61_R 94_E 1.267 0.63
81_R 88_N 1.264 0.63
191_R 273_I 1.262 0.63
84_D 87_L 1.259 0.63
472_G 488_I 1.257 0.63
77_L 117_K 1.251 0.62
389_M 404_E 1.251 0.62
419_Q 423_E 1.242 0.61
10_E 76_T 1.24 0.61
224_K 283_F 1.235 0.61
420_A 423_E 1.234 0.60
237_A 283_F 1.217 0.59
66_D 84_D 1.216 0.59
134_E 185_S 1.212 0.58
70_Q 73_K 1.211 0.58
424_S 450_A 1.211 0.58
469_D 488_I 1.206 0.58
468_A 473_L 1.201 0.57
47_V 58_T 1.199 0.57
438_E 441_D 1.196 0.57
103_D 107_T 1.195 0.57
21_E 86_S 1.194 0.57
443_D 450_A 1.19 0.56
130_F 136_E 1.189 0.56
118_V 157_N 1.188 0.56
152_L 181_G 1.187 0.56
372_V 375_E 1.186 0.56
437_L 489_C 1.184 0.56
64_V 77_L 1.179 0.55
424_S 427_D 1.176 0.55
454_C 458_D 1.176 0.55
116_Q 191_R 1.175 0.55
87_L 93_V 1.171 0.54
373_L 378_F 1.164 0.54
21_E 389_M 1.163 0.54
216_I 289_A 1.163 0.54
236_I 262_V 1.163 0.54
130_F 312_G 1.16 0.53
162_I 196_F 1.156 0.53
2_N 197_V 1.156 0.53
468_A 475_D 1.155 0.53
130_F 292_D 1.154 0.53
471_E 489_C 1.154 0.53
44_D 61_R 1.152 0.53
89_L 201_K 1.152 0.53
431_A 457_E 1.136 0.51
173_F 179_V 1.135 0.51
476_E 484_A 1.134 0.51
162_I 453_V 1.134 0.51
420_A 476_E 1.132 0.51
393_L 403_V 1.126 0.50
133_H 136_E 1.125 0.50
113_V 123_R 1.123 0.50
442_R 446_F 1.122 0.50
342_D 346_A 1.119 0.49
81_R 86_S 1.117 0.49
30_A 334_W 1.117 0.49
365_I 398_L 1.115 0.49
288_M 307_I 1.112 0.49
405_A 408_E 1.111 0.49
279_N 308_A 1.111 0.49
166_D 216_I 1.11 0.49
85_E 461_E 1.11 0.49
148_D 240_T 1.109 0.48
488_I 492_G 1.109 0.48
456_L 464_I 1.109 0.48
110_A 229_D 1.108 0.48
213_V 273_I 1.106 0.48
392_L 424_S 1.104 0.48
291_A 317_A 1.103 0.48
218_E 313_N 1.1 0.48
435_L 457_E 1.099 0.47
136_E 397_G 1.098 0.47
163_L 166_D 1.098 0.47
452_G 480_A 1.098 0.47
162_I 173_F 1.096 0.47
445_A 481_L 1.096 0.47
462_Q 469_D 1.095 0.47
456_L 480_A 1.094 0.47
10_E 14_N 1.092 0.47
205_L 226_A 1.091 0.47
455_T 458_D 1.09 0.47
57_D 193_A 1.089 0.46
68_V 78_E 1.088 0.46
10_E 25_E 1.088 0.46
171_E 192_G 1.087 0.46
358_T 361_K 1.084 0.46
144_K 240_T 1.082 0.46
431_A 458_D 1.08 0.46
117_K 180_R 1.079 0.46
140_G 151_S 1.078 0.45
34_A 326_R 1.077 0.45
220_V 271_I 1.077 0.45
405_A 409_R 1.077 0.45
305_M 334_W 1.076 0.45
176_G 484_A 1.073 0.45
18_L 262_V 1.072 0.45
204_M 219_E 1.07 0.45
307_I 325_V 1.07 0.45
151_S 274_V 1.07 0.45
373_L 387_V 1.069 0.45
64_V 87_L 1.067 0.44
77_L 84_D 1.065 0.44
461_E 491_F 1.063 0.44
63_L 320_R 1.062 0.44
183_L 186_V 1.062 0.44
179_V 273_I 1.06 0.44
436_N 461_E 1.059 0.44
36_K 109_T 1.058 0.44
310_E 341_V 1.058 0.44
271_I 392_L 1.057 0.43
252_V 336_L 1.057 0.43
398_L 409_R 1.056 0.43
470_I 489_C 1.053 0.43
75_I 93_V 1.051 0.43
267_G 320_R 1.051 0.43
136_E 186_V 1.05 0.43
200_S 230_P 1.049 0.43
128_D 346_A 1.049 0.43
216_I 236_I 1.048 0.43
66_D 76_T 1.045 0.42
186_V 288_M 1.045 0.42
240_T 294_A 1.043 0.42
475_D 479_G 1.043 0.42
435_L 440_V 1.037 0.42
362_Y 417_I 1.036 0.41
427_D 436_N 1.034 0.41
179_V 371_T 1.034 0.41
367_E 371_T 1.033 0.41
436_N 477_K 1.033 0.41
209_F 273_I 1.033 0.41
126_V 356_I 1.03 0.41
77_L 80_A 1.029 0.41
220_V 245_I 1.029 0.41
55_D 138_I 1.029 0.41
367_E 384_L 1.029 0.41
325_V 335_E 1.024 0.40
438_E 477_K 1.023 0.40
142_V 145_V 1.022 0.40
433_D 456_L 1.02 0.40
25_E 293_V 1.018 0.40
430_P 446_F 1.018 0.40
425_L 443_D 1.017 0.40
20_R 166_D 1.017 0.40
461_E 466_D 1.015 0.39
107_T 114_I 1.013 0.39
438_E 488_I 1.013 0.39
88_N 440_V 1.012 0.39
113_V 121_A 1.01 0.39
451_R 469_D 1.009 0.39
102_F 357_D 1.006 0.39
184_Y 196_F 1.006 0.39
453_V 477_K 1.005 0.39
213_V 223_I 1.004 0.38
227_A 387_V 1.003 0.38
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4mtnA10.81621000.338Contact Map0.413
1k0rA20.68281000.374Contact Map0.496
1hh2P10.69291000.394Contact Map0.451
2asbA10.48891000.543Contact Map0.559
2kwpA10.26061000.783Contact Map0.52
2cxcA10.28691000.79Contact Map0.258
2qndA20.288999.50.921Contact Map0.181
1wcnA10.139499.40.923Contact Map0.427
1u9lA20.141499.20.929Contact Map0.637
4aybE10.30198.60.944Contact Map0.556
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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