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OPENSEQ.org
RSSB - Regulator of RpoS
UniProt: P0AEV1 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12121
Length: 337 (302)
Sequences: 781
Seq/Len: 2.59
RSSB
Paralog alert: 0.25 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
39_D 63_R 2.467 1.00
54_L 124_L 2.311 1.00
41_V 63_R 2.257 1.00
59_I 84_V 2.201 1.00
40_G 64_M 2.185 1.00
9_Q 33_T 2.168 1.00
57_C 69_L 2.166 1.00
15_D 66_G 2.124 1.00
245_L 278_V 2.054 1.00
65_N 68_K 2.026 1.00
262_P 282_L 2.015 0.99
61_M 64_M 1.963 0.99
40_G 61_M 1.942 0.99
38_A 42_D 1.898 0.99
20_R 36_L 1.882 0.99
37_A 46_L 1.86 0.99
53_D 81_P 1.816 0.99
205_V 282_L 1.802 0.99
61_M 66_G 1.79 0.99
15_D 58_D 1.769 0.98
58_D 66_G 1.765 0.98
175_Y 188_L 1.752 0.98
200_F 266_G 1.724 0.98
15_D 61_M 1.694 0.98
14_E 20_R 1.682 0.98
197_D 269_H 1.677 0.97
20_R 38_A 1.675 0.97
157_L 183_K 1.664 0.97
14_E 61_M 1.634 0.97
74_R 103_E 1.608 0.97
43_A 69_L 1.604 0.96
185_G 204_D 1.576 0.96
44_L 64_M 1.571 0.96
66_G 108_K 1.566 0.96
13_V 43_A 1.553 0.96
47_L 73_I 1.547 0.95
58_D 87_A 1.535 0.95
103_E 125_Y 1.535 0.95
70_L 82_V 1.527 0.95
266_G 275_L 1.521 0.95
194_S 197_D 1.512 0.95
70_L 84_V 1.51 0.95
83_L 120_V 1.504 0.94
62_P 66_G 1.483 0.94
84_V 102_V 1.483 0.94
211_N 283_N 1.471 0.93
68_K 72_H 1.469 0.93
269_H 272_L 1.453 0.93
39_D 61_M 1.442 0.93
84_V 97_A 1.429 0.92
203_L 261_F 1.426 0.92
95_A 98_L 1.425 0.92
20_R 61_M 1.423 0.92
97_A 122_A 1.422 0.92
45_E 48_G 1.407 0.91
12_I 34_T 1.403 0.91
186_L 204_D 1.396 0.91
81_P 105_V 1.392 0.91
14_E 19_F 1.39 0.91
40_G 66_G 1.387 0.91
9_Q 35_V 1.381 0.90
15_D 87_A 1.376 0.90
45_E 49_G 1.371 0.90
23_L 83_L 1.366 0.90
61_M 65_N 1.363 0.90
186_L 210_H 1.354 0.89
58_D 61_M 1.351 0.89
241_L 278_V 1.346 0.89
64_M 68_K 1.342 0.89
37_A 40_G 1.295 0.86
58_D 108_K 1.284 0.85
205_V 218_L 1.28 0.85
150_P 217_L 1.28 0.85
42_D 291_H 1.28 0.85
153_A 183_K 1.277 0.85
66_G 87_A 1.272 0.85
19_F 69_L 1.245 0.83
20_R 39_D 1.239 0.83
184_P 203_L 1.223 0.81
162_P 189_D 1.222 0.81
173_V 198_L 1.22 0.81
40_G 62_P 1.216 0.81
80_T 85_I 1.205 0.80
97_A 118_E 1.195 0.79
122_A 125_Y 1.189 0.79
87_A 108_K 1.178 0.78
55_M 106_L 1.178 0.78
205_V 298_G 1.174 0.78
196_N 271_E 1.163 0.77
186_L 211_N 1.154 0.76
215_A 260_Q 1.15 0.76
211_N 215_A 1.128 0.74
58_D 62_P 1.127 0.73
30_L 117_R 1.126 0.73
184_P 226_L 1.125 0.73
161_Q 188_L 1.124 0.73
187_V 203_L 1.123 0.73
98_L 118_E 1.12 0.73
103_E 116_L 1.118 0.73
156_L 214_L 1.118 0.73
73_I 80_T 1.115 0.72
67_L 84_V 1.112 0.72
284_A 287_N 1.112 0.72
74_R 82_V 1.105 0.71
118_E 122_A 1.104 0.71
47_L 52_P 1.103 0.71
57_C 82_V 1.103 0.71
193_L 199_A 1.093 0.70
42_D 305_G 1.089 0.70
15_D 62_P 1.086 0.69
84_V 123_C 1.084 0.69
13_V 40_G 1.072 0.68
59_I 97_A 1.071 0.68
40_G 44_L 1.067 0.68
115_R 118_E 1.063 0.67
179_V 186_L 1.062 0.67
14_E 107_L 1.062 0.67
12_I 23_L 1.06 0.67
222_L 261_F 1.058 0.67
90_N 93_D 1.052 0.66
14_E 108_K 1.051 0.66
204_D 210_H 1.048 0.66
157_L 262_P 1.044 0.65
81_P 123_C 1.044 0.65
15_D 40_G 1.039 0.65
252_L 301_L 1.037 0.64
103_E 122_A 1.035 0.64
20_R 109_P 1.034 0.64
241_L 245_L 1.033 0.64
249_N 302_G 1.033 0.64
177_Q 188_L 1.031 0.64
23_L 56_I 1.031 0.64
184_P 282_L 1.03 0.64
14_E 66_G 1.029 0.63
213_V 229_E 1.02 0.62
39_D 64_M 1.019 0.62
193_L 265_V 1.019 0.62
157_L 282_L 1.018 0.62
211_N 219_L 1.016 0.62
84_V 94_I 1.006 0.61
127_S 131_S 1.002 0.60
205_V 262_P 1 0.60
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3eq2A20.98521000.313Contact Map0.483
3f79A60.59641000.625Contact Map0.632
3ke6A20.56681000.656Contact Map0.492
4l4uA10.86941000.68Contact Map0.476
1ny5A20.8221000.683Contact Map0.578
3dzdA20.8191000.686Contact Map0.548
3n0rA10.38581000.689Contact Map0.447
3breA20.9111000.705Contact Map0.523
2ayxA10.39171000.706Contact Map0.279
3lufA20.41251000.71Contact Map0.571
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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