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OPENSEQ.org
RSEP - Regulator of sigma E protease
UniProt: P0AEH1 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12436
Length: 450 (448)
Sequences: 1339
Seq/Len: 2.99
RSEP
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
35_V 100_I 6.881 1.00
92_K 96_Q 4.695 1.00
29_V 99_A 4.07 1.00
276_I 285_L 3.507 1.00
359_S 363_S 3.474 1.00
248_K 275_E 3.394 1.00
232_N 236_S 3.099 1.00
109_F 391_G 2.969 1.00
27_F 59_Y 2.863 1.00
102_A 106_V 2.815 1.00
271_S 286_T 2.814 1.00
93_S 96_Q 2.683 1.00
276_I 283_L 2.611 1.00
277_E 280_G 2.442 1.00
56_G 87_H 2.401 1.00
322_G 325_N 2.395 1.00
28_W 32_R 2.394 1.00
245_R 277_E 2.36 1.00
268_P 300_G 2.345 1.00
267_N 270_K 2.285 1.00
98_A 406_L 2.182 1.00
287_L 302_V 2.163 1.00
229_V 233_S 2.093 1.00
98_A 410_A 2.062 1.00
275_E 282_P 2.062 1.00
101_I 409_L 2.023 1.00
18_L 390_L 1.998 1.00
21_V 111_F 1.963 1.00
47_A 62_A 1.933 0.99
237_K 290_E 1.915 0.99
53_D 56_G 1.909 0.99
111_F 115_A 1.887 0.99
275_E 284_S 1.885 0.99
30_A 72_M 1.866 0.99
30_A 37_V 1.841 0.99
31_R 56_G 1.83 0.99
24_F 28_W 1.83 0.99
94_V 98_A 1.816 0.99
250_D 267_N 1.795 0.99
289_P 302_V 1.762 0.99
322_G 326_A 1.757 0.99
109_F 113_I 1.745 0.99
14_A 379_Y 1.742 0.99
38_E 73_L 1.741 0.99
248_K 251_G 1.74 0.99
88_A 91_N 1.738 0.99
229_V 242_A 1.714 0.98
39_R 60_V 1.709 0.98
35_V 72_M 1.693 0.98
33_C 96_Q 1.689 0.98
49_W 61_I 1.688 0.98
101_I 406_L 1.63 0.98
105_P 395_L 1.628 0.98
249_V 260_F 1.624 0.98
27_F 40_F 1.61 0.97
240_L 274_L 1.595 0.97
267_N 272_L 1.589 0.97
273_A 284_S 1.588 0.97
260_F 274_L 1.562 0.97
247_V 275_E 1.547 0.97
102_A 105_P 1.533 0.96
55_L 59_Y 1.529 0.96
29_V 103_A 1.522 0.96
14_A 383_L 1.521 0.96
118_L 122_I 1.52 0.96
94_V 413_K 1.506 0.96
48_L 63_L 1.496 0.96
432_S 436_V 1.492 0.95
240_L 285_L 1.485 0.95
31_R 55_L 1.477 0.95
116_Y 384_A 1.474 0.95
244_D 278_R 1.464 0.95
117_W 330_A 1.464 0.95
226_L 246_I 1.45 0.94
50_R 60_V 1.449 0.94
236_S 241_Q 1.444 0.94
415_K 419_V 1.442 0.94
408_F 427_C 1.441 0.94
11_F 365_A 1.439 0.94
41_S 60_V 1.436 0.94
39_R 58_E 1.429 0.94
259_T 262_M 1.428 0.94
72_M 100_I 1.401 0.93
75_E 90_N 1.393 0.93
355_L 441_L 1.389 0.93
231_P 236_S 1.384 0.92
247_V 282_P 1.38 0.92
94_V 406_L 1.38 0.92
98_A 413_K 1.371 0.92
82_P 85_R 1.368 0.92
250_D 263_L 1.35 0.91
263_L 272_L 1.349 0.91
105_P 391_G 1.334 0.91
41_S 62_A 1.33 0.90
159_T 164_A 1.328 0.90
439_M 443_L 1.326 0.90
140_A 143_A 1.323 0.90
106_V 110_I 1.321 0.90
249_V 263_L 1.317 0.90
51_R 54_K 1.309 0.89
38_E 71_K 1.291 0.89
277_E 282_P 1.288 0.88
29_V 33_C 1.286 0.88
37_V 72_M 1.283 0.88
436_V 440_G 1.277 0.88
47_A 50_R 1.276 0.88
273_A 283_L 1.275 0.88
102_A 395_L 1.271 0.88
327_I 331_T 1.27 0.87
101_I 405_H 1.268 0.87
29_V 32_R 1.267 0.87
85_R 91_N 1.265 0.87
47_A 58_E 1.26 0.87
330_A 334_T 1.254 0.87
112_A 388_V 1.254 0.87
155_D 172_K 1.243 0.86
323_P 326_A 1.24 0.86
73_L 88_A 1.235 0.85
44_F 66_L 1.232 0.85
339_K 343_S 1.225 0.85
255_T 259_T 1.221 0.85
12_I 16_G 1.22 0.84
335_W 338_M 1.218 0.84
254_L 262_M 1.216 0.84
108_N 390_L 1.213 0.84
391_G 395_L 1.21 0.84
411_I 419_V 1.205 0.83
80_V 86_H 1.204 0.83
84_L 87_H 1.203 0.83
33_C 99_A 1.202 0.83
341_T 389_N 1.192 0.83
226_L 240_L 1.189 0.82
21_V 107_A 1.189 0.82
53_D 59_Y 1.177 0.81
257_W 261_V 1.173 0.81
338_M 342_V 1.173 0.81
272_L 289_P 1.166 0.81
119_V 381_P 1.166 0.81
44_F 393_I 1.166 0.81
345_L 349_I 1.164 0.80
81_V 84_L 1.162 0.80
415_K 420_S 1.159 0.80
50_R 58_E 1.156 0.80
11_F 391_G 1.151 0.79
235_A 239_G 1.146 0.79
120_F 329_E 1.144 0.79
348_L 357_N 1.143 0.79
256_Q 259_T 1.143 0.79
94_V 409_L 1.133 0.78
225_V 245_R 1.132 0.78
410_A 414_I 1.129 0.78
333_K 336_Q 1.126 0.77
249_V 302_V 1.125 0.77
94_V 101_I 1.124 0.77
331_T 334_T 1.119 0.77
389_N 393_I 1.119 0.77
273_A 286_T 1.118 0.77
153_A 156_G 1.108 0.76
11_F 19_I 1.108 0.76
72_M 75_E 1.105 0.75
269_G 300_G 1.105 0.75
95_G 413_K 1.104 0.75
10_S 372_A 1.103 0.75
18_L 387_S 1.101 0.75
292_K 301_F 1.099 0.75
444_F 447_F 1.094 0.74
250_D 273_A 1.09 0.74
240_L 276_I 1.088 0.74
440_G 443_L 1.086 0.74
183_A 190_R 1.086 0.74
249_V 272_L 1.086 0.74
324_F 328_V 1.084 0.73
415_K 418_P 1.082 0.73
19_I 410_A 1.076 0.73
424_Q 428_Y 1.072 0.72
47_A 60_V 1.069 0.72
263_L 267_N 1.069 0.72
153_A 181_T 1.068 0.72
417_G 420_S 1.066 0.72
329_E 332_D 1.066 0.72
386_I 435_L 1.06 0.71
41_S 45_G 1.053 0.70
419_V 423_V 1.052 0.70
426_F 430_I 1.052 0.70
342_V 392_I 1.05 0.70
240_L 283_L 1.045 0.69
252_Q 263_L 1.044 0.69
23_E 26_H 1.031 0.68
105_P 108_N 1.028 0.68
11_F 15_L 1.025 0.67
406_L 409_L 1.025 0.67
420_S 423_V 1.025 0.67
102_A 406_L 1.025 0.67
278_R 283_L 1.023 0.67
98_A 409_L 1.021 0.67
181_T 190_R 1.019 0.67
7_D 10_S 1.019 0.67
22_H 26_H 1.016 0.66
342_V 346_G 1.016 0.66
30_A 100_I 1.016 0.66
335_W 339_K 1.015 0.66
97_R 330_A 1.012 0.66
19_I 42_I 1.007 0.65
291_S 300_G 1.002 0.65
392_I 396_F 1.001 0.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1p1dA10.411199.90.812Contact Map0.497
3rleA10.4499.90.813Contact Map0.596
4kfvA10.442299.90.82Contact Map0.641
4a8cA120.564499.90.831Contact Map0.177
3pv2A40.548999.90.836Contact Map0.839
3gslA20.397899.90.837Contact Map0.436
1ky9A20.528999.90.84Contact Map0.486
3r0hA80.433399.90.84Contact Map0.421
1w9eA20.364499.80.846Contact Map0.38
2qt5A20.397899.80.846Contact Map0.574
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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