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OPENSEQ.org

RSMD - Ribosomal RNA small subunit methyltransferase D
UniProt: P0ADX9 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10343
Length: 198 (181)
Sequences: 1860
Seq/Len: 10.28

RSMD
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
67_G 80_L 3.727 1.00
14_I 68_L 3.385 1.00
68_L 95_L 2.896 1.00
40_R 65_A 2.689 1.00
148_L 152_A 2.486 1.00
43_L 184_Y 2.453 1.00
56_C 66_L 2.403 1.00
158_S 185_R 2.395 1.00
55_Q 77_G 2.37 1.00
25_P 93_K 2.319 1.00
19_W 100_A 2.288 1.00
71_L 103_A 2.244 1.00
82_E 88_S 2.218 1.00
79_T 121_H 2.198 1.00
41_E 45_N 2.132 1.00
22_R 97_T 2.049 1.00
42_T 179_A 2.01 1.00
173_L 187_Y 1.974 1.00
85_R 107_N 1.961 1.00
39_V 182_V 1.934 1.00
61_A 80_L 1.859 1.00
18_Q 102_N 1.805 1.00
32_L 87_V 1.772 1.00
71_L 78_A 1.759 1.00
71_L 80_L 1.744 1.00
142_L 152_A 1.741 1.00
122_N 149_A 1.727 1.00
46_W 186_L 1.709 1.00
60_F 138_T 1.676 1.00
95_L 103_A 1.662 1.00
124_V 152_A 1.645 1.00
13_R 37_D 1.635 1.00
88_S 105_V 1.632 1.00
95_L 105_V 1.572 1.00
59_C 81_I 1.535 1.00
77_G 104_R 1.53 1.00
57_L 121_H 1.526 1.00
134_L 138_T 1.501 1.00
22_R 99_K 1.493 1.00
66_L 125_F 1.429 1.00
42_T 45_N 1.416 1.00
71_L 95_L 1.41 1.00
80_L 103_A 1.405 0.99
176_E 183_A 1.405 0.99
160_V 178_V 1.401 0.99
85_R 89_Q 1.397 0.99
108_S 113_F 1.394 0.99
178_V 183_A 1.391 0.99
67_G 78_A 1.39 0.99
89_Q 93_K 1.388 0.99
35_T 157_E 1.372 0.99
113_F 117_K 1.35 0.99
82_E 87_V 1.344 0.99
43_L 66_L 1.342 0.99
19_W 98_L 1.34 0.99
60_F 109_N 1.324 0.99
149_A 152_A 1.324 0.99
173_L 185_R 1.318 0.99
79_T 106_V 1.313 0.99
35_T 127_D 1.312 0.99
46_W 184_Y 1.295 0.99
81_I 113_F 1.288 0.99
124_V 142_L 1.282 0.99
15_I 41_E 1.243 0.98
39_V 42_T 1.239 0.98
61_A 67_G 1.233 0.98
57_L 124_V 1.209 0.98
24_L 94_N 1.172 0.98
153_L 174_H 1.16 0.97
46_W 175_R 1.155 0.97
131_R 159_E 1.149 0.97
131_R 161_E 1.147 0.97
51_I 54_A 1.138 0.97
143_E 189_R 1.126 0.97
56_C 125_F 1.122 0.97
15_I 37_D 1.102 0.96
172_S 190_E 1.082 0.96
56_C 70_A 1.078 0.95
59_C 110_A 1.073 0.95
51_I 123_I 1.072 0.95
140_N 144_D 1.07 0.95
61_A 91_L 1.049 0.94
57_L 79_T 1.04 0.94
88_S 107_N 1.04 0.94
88_S 91_L 1.03 0.94
12_I 26_V 1.025 0.93
173_L 176_E 1.019 0.93
159_E 181_Q 1.011 0.93
32_L 90_Q 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2fpoA611000.572Contact Map0.772
1ws6A10.84341000.574Contact Map0.733
2iftA20.98991000.59Contact Map0.701
2fhpA20.924299.90.608Contact Map0.816
4dcmA10.929399.90.628Contact Map0.519
4bluA20.919299.90.646Contact Map0.304
2esrA20.883899.90.647Contact Map0.776
2oo3A10.939499.90.651Contact Map0.316
3p9nA10.929399.90.652Contact Map0.739
2dulA10.89999.80.71Contact Map0.595

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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