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OPENSEQ.org

NIFU - NifU-like protein
UniProt: P0ACD4 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13395
Length: 128 (125)
Sequences: 1543
Seq/Len: 12.34

NIFU
Paralog alert: 0.07 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
48_N 54_E 4.312 1.00
15_R 75_E 3.266 1.00
20_F 57_R 3.024 1.00
16_N 78_K 2.876 1.00
24_D 46_K 2.589 1.00
81_S 84_E 2.4 1.00
55_D 78_K 2.162 1.00
9_D 15_R 2.132 1.00
19_S 59_K 2.094 1.00
16_N 74_T 2.028 1.00
80_K 84_E 2.001 1.00
46_K 54_E 1.941 1.00
53_I 85_A 1.9 1.00
10_H 15_R 1.878 1.00
46_K 55_D 1.773 1.00
22_N 46_K 1.756 1.00
52_I 81_S 1.754 1.00
44_Q 57_R 1.675 1.00
82_L 118_I 1.599 1.00
31_M 42_K 1.594 1.00
18_G 57_R 1.55 1.00
10_H 71_S 1.512 1.00
19_S 44_Q 1.466 1.00
123_S 126_E 1.443 1.00
32_V 113_A 1.439 1.00
72_L 96_E 1.427 1.00
19_S 42_K 1.422 1.00
119_A 123_S 1.409 1.00
47_V 82_L 1.39 0.99
58_F 74_T 1.388 0.99
70_S 110_A 1.376 0.99
29_S 44_Q 1.372 0.99
56_A 77_V 1.368 0.99
77_V 114_I 1.34 0.99
28_G 45_I 1.339 0.99
39_D 106_C 1.321 0.99
71_S 74_T 1.313 0.99
72_L 76_W 1.288 0.99
122_K 126_E 1.279 0.99
83_D 86_Q 1.261 0.99
94_A 104_I 1.257 0.99
122_K 125_R 1.242 0.98
14_P 71_S 1.207 0.98
10_H 14_P 1.153 0.97
86_Q 118_I 1.142 0.97
20_F 44_Q 1.132 0.97
7_V 65_S 1.127 0.97
3_Y 7_V 1.113 0.96
16_N 71_S 1.112 0.96
63_C 106_C 1.107 0.96
119_A 122_K 1.099 0.96
17_V 60_T 1.069 0.95
15_R 71_S 1.042 0.94
84_E 87_A 1.035 0.94
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3lvlA111000.028Contact Map0.821
2z7eA30.96881000.046Contact Map0.906
4eb5C20.96091000.049Contact Map0.87
1su0B10.96091000.078Contact Map0.865
1xjsA10.93751000.083Contact Map0.654
2qq4A100.89841000.109Contact Map0.914
3g0mA10.87596.70.812Contact Map0.515
1ni7A10.890678.60.881Contact Map0.446
4lw4C20.867274.90.884Contact Map0.457
1wloA10.87564.10.893Contact Map0.565

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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