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OPENSEQ.org
HEM2 - Delta-aminolevulinic acid dehydratase
UniProt: P0ACB2 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10428
Length: 324 (317)
Sequences: 1458
Seq/Len: 4.60
HEM2
Paralog alert: 0.03 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
257_R 261_E 3.444 1.00
91_D 94_R 3.37 1.00
30_D 302_K 3.368 1.00
226_R 258_E 3.302 1.00
233_S 259_L 3.26 1.00
64_E 315_L 3.09 1.00
87_E 126_S 3.03 1.00
291_E 320_K 2.957 1.00
100_A 153_V 2.787 1.00
117_S 154_A 2.689 1.00
166_A 171_Q 2.684 1.00
237_E 265_L 2.662 1.00
298_L 317_L 2.565 1.00
107_K 160_D 2.545 1.00
249_A 301_I 2.473 1.00
103_S 115_V 2.465 1.00
226_R 255_I 2.394 1.00
233_S 244_L 2.39 1.00
139_D 142_A 2.298 1.00
294_V 320_K 2.237 1.00
22_E 222_N 2.235 1.00
152_V 178_A 2.178 1.00
137_G 186_D 2.175 1.00
66_E 70_N 2.165 1.00
256_V 269_A 2.16 1.00
194_T 233_S 2.121 1.00
156_A 184_F 2.117 1.00
46_A 54_M 2.07 1.00
11_L 21_F 2.068 1.00
268_G 308_L 2.058 1.00
44_Y 54_M 2.029 1.00
289_D 292_K 2.018 1.00
93_W 146_N 1.955 1.00
280_K 290_E 1.948 1.00
260_R 307_D 1.924 1.00
8_P 235_L 1.919 1.00
281_F 287_A 1.914 1.00
15_P 19_A 1.889 1.00
75_S 116_M 1.87 1.00
95_E 156_A 1.865 1.00
67_R 319_E 1.835 1.00
243_C 268_G 1.825 1.00
48_E 206_E 1.804 1.00
86_D 90_S 1.794 1.00
69_A 110_V 1.782 1.00
44_Y 56_I 1.781 1.00
60_H 63_R 1.757 1.00
66_E 110_V 1.753 1.00
45_K 55_R 1.732 0.99
234_L 238_A 1.719 0.99
47_V 53_V 1.716 0.99
49_A 206_E 1.715 0.99
156_A 182_A 1.712 0.99
132_V 146_N 1.705 0.99
148_G 178_A 1.705 0.99
32_V 308_L 1.662 0.99
271_Q 313_F 1.659 0.99
258_E 261_E 1.657 0.99
251_A 300_S 1.645 0.99
259_L 267_I 1.635 0.99
62_A 110_V 1.629 0.99
250_G 271_Q 1.624 0.99
103_S 117_S 1.621 0.99
63_R 66_E 1.609 0.99
93_W 149_K 1.608 0.99
200_F 297_S 1.599 0.99
31_L 311_S 1.582 0.99
44_Y 60_H 1.543 0.99
19_A 29_N 1.529 0.98
227_R 258_E 1.521 0.98
19_A 74_R 1.509 0.98
290_E 294_V 1.502 0.98
190_M 243_C 1.487 0.98
31_L 317_L 1.459 0.98
6_Q 231_R 1.4 0.97
227_R 260_R 1.399 0.97
117_S 159_A 1.397 0.97
121_F 150_Q 1.391 0.97
316_D 319_E 1.384 0.97
58_E 105_I 1.383 0.97
249_A 269_A 1.38 0.97
77_M 310_F 1.364 0.96
237_E 244_L 1.362 0.96
66_E 109_T 1.36 0.96
25_T 29_N 1.36 0.96
77_M 163_A 1.356 0.96
279_I 297_S 1.355 0.96
161_F 243_C 1.354 0.96
42_D 60_H 1.353 0.96
65_I 76_V 1.342 0.96
280_K 316_D 1.339 0.96
40_E 59_K 1.335 0.96
61_L 106_C 1.326 0.95
76_V 115_V 1.316 0.95
234_L 263_T 1.315 0.95
62_A 109_T 1.313 0.95
161_F 188_A 1.305 0.95
24_T 301_I 1.3 0.95
244_L 265_L 1.298 0.95
200_F 279_I 1.298 0.95
295_L 317_L 1.294 0.95
178_A 182_A 1.29 0.94
43_D 57_P 1.285 0.94
65_I 110_V 1.274 0.94
283_A 294_V 1.274 0.94
119_T 162_I 1.27 0.94
76_V 113_M 1.265 0.94
105_I 108_Q 1.255 0.93
65_I 113_M 1.251 0.93
253_L 301_I 1.246 0.93
294_V 313_F 1.243 0.93
86_D 89_G 1.242 0.93
39_E 101_R 1.237 0.93
226_R 257_R 1.232 0.92
64_E 68_I 1.211 0.92
67_R 315_L 1.204 0.91
97_G 100_A 1.2 0.91
298_L 301_I 1.199 0.91
151_A 162_I 1.187 0.90
244_L 259_L 1.184 0.90
250_G 297_S 1.182 0.90
33_L 68_I 1.179 0.90
96_D 101_R 1.173 0.90
148_G 174_A 1.172 0.90
16_A 114_I 1.167 0.89
127_H 133_L 1.153 0.89
279_I 288_I 1.152 0.89
65_I 106_C 1.147 0.88
114_I 161_F 1.147 0.88
271_Q 297_S 1.145 0.88
290_E 316_D 1.127 0.87
105_I 109_T 1.127 0.87
283_A 288_I 1.123 0.87
49_A 287_A 1.121 0.87
73_I 318_A 1.119 0.87
62_A 66_E 1.119 0.87
120_C 130_C 1.118 0.87
33_L 53_V 1.109 0.86
116_M 163_A 1.097 0.85
250_G 300_S 1.088 0.85
130_C 216_R 1.086 0.84
193_S 233_S 1.082 0.84
69_A 113_M 1.081 0.84
38_E 43_D 1.08 0.84
104_R 156_A 1.072 0.83
200_F 251_A 1.07 0.83
230_I 259_L 1.069 0.83
194_T 244_L 1.06 0.82
291_E 294_V 1.059 0.82
152_V 182_A 1.056 0.82
234_L 262_R 1.056 0.82
99_V 124_Y 1.055 0.82
288_I 293_V 1.052 0.82
14_S 188_A 1.05 0.82
42_D 59_K 1.038 0.80
68_I 315_L 1.037 0.80
147_L 166_A 1.033 0.80
199_S 293_V 1.031 0.80
295_L 320_K 1.025 0.79
120_C 216_R 1.025 0.79
50_M 277_A 1.024 0.79
26_L 298_L 1.024 0.79
283_A 289_D 1.021 0.79
28_L 31_L 1.014 0.78
237_E 263_T 1.013 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1w1zA20.9938100-0.007Contact Map0.835
1l6sA20.9969100-0.006Contact Map0.791
1w5qA20.99691000.003Contact Map0.791
3obkA80.99691000.003Contact Map0.817
1h7nA111000.036Contact Map0.703
1pv8A20.99691000.043Contact Map0.776
3labA20.601995.90.957Contact Map0.52
1wbhA30.586495.70.958Contact Map0.569
3b8iA60.743894.20.961Contact Map0.453
1mxsA10.611192.90.963Contact Map0.517
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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