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OPENSEQ.org
APBE - Thiamine biosynthesis lipoprotein ApbE
UniProt: P0AB85 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12073
Length: 351 (329)
Sequences: 1642
Seq/Len: 4.99
APBE
Paralog alert: 0.18 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
84_A 100_S 4.905 1.00
68_Q 71_A 3.489 1.00
146_Q 150_D 3.275 1.00
121_M 185_T 3.246 1.00
117_T 304_G 2.683 1.00
114_G 123_I 2.607 1.00
294_T 297_E 2.529 1.00
104_A 163_V 2.496 1.00
120_A 307_V 2.446 1.00
157_G 160_H 2.433 1.00
114_G 158_L 2.432 1.00
318_R 340_P 2.422 1.00
194_H 197_R 2.387 1.00
72_D 191_A 2.377 1.00
76_L 106_I 2.312 1.00
68_Q 194_H 2.191 1.00
292_A 298_A 2.187 1.00
250_H 293_P 2.145 1.00
113_I 300_A 2.132 1.00
131_L 152_M 2.111 1.00
290_V 305_L 2.109 1.00
164_I 167_S 2.044 1.00
254_T 287_S 1.978 1.00
207_Y 218_R 1.947 1.00
73_D 80_K 1.872 1.00
214_A 253_S 1.857 1.00
108_T 165_N 1.855 1.00
109_T 296_L 1.839 1.00
234_P 258_Y 1.835 1.00
145_S 148_Q 1.811 1.00
65_I 195_L 1.784 1.00
245_V 291_I 1.78 1.00
189_G 299_D 1.778 1.00
308_L 316_V 1.769 1.00
193_D 294_T 1.758 1.00
288_V 326_M 1.738 1.00
47_R 210_S 1.729 1.00
104_A 165_N 1.723 1.00
305_L 313_A 1.712 1.00
200_E 207_Y 1.71 1.00
85_L 103_M 1.71 1.00
155_K 178_D 1.691 0.99
109_T 112_R 1.686 0.99
74_Q 80_K 1.683 0.99
64_K 202_E 1.665 0.99
68_Q 72_D 1.663 0.99
301_W 322_L 1.657 0.99
107_V 170_Q 1.655 0.99
253_S 295_A 1.631 0.99
263_E 266_G 1.625 0.99
76_L 187_G 1.61 0.99
96_P 171_Y 1.591 0.99
313_A 326_M 1.579 0.99
68_Q 198_L 1.569 0.99
267_K 282_E 1.555 0.99
124_T 156_T 1.541 0.99
198_L 202_E 1.522 0.99
132_W 280_P 1.519 0.99
106_I 296_L 1.519 0.99
86_M 90_D 1.512 0.99
309_G 313_A 1.504 0.99
64_K 198_L 1.503 0.99
124_T 157_G 1.501 0.99
125_V 129_V 1.489 0.98
214_A 295_A 1.481 0.98
122_D 157_G 1.474 0.98
208_L 217_S 1.469 0.98
123_I 181_V 1.463 0.98
130_N 180_Y 1.46 0.98
196_A 200_E 1.459 0.98
72_D 76_L 1.453 0.98
71_A 102_A 1.444 0.98
312_K 315_E 1.435 0.98
75_L 103_M 1.434 0.98
232_Q 259_R 1.405 0.97
285_L 326_M 1.393 0.97
242_Q 327_I 1.382 0.97
84_A 103_M 1.38 0.97
196_A 207_Y 1.371 0.97
110_S 186_V 1.36 0.97
75_L 100_S 1.355 0.97
200_E 218_R 1.343 0.96
285_L 309_G 1.342 0.96
116_K 301_W 1.339 0.96
17_F 20_C 1.334 0.96
118_D 312_K 1.328 0.96
185_T 303_T 1.324 0.96
153_K 278_G 1.316 0.96
77_S 80_K 1.314 0.96
328_T 332_D 1.312 0.96
110_S 123_I 1.302 0.95
316_V 320_E 1.299 0.95
301_W 316_V 1.299 0.95
324_V 339_S 1.288 0.95
149_I 279_R 1.281 0.95
69_L 211_V 1.279 0.95
255_S 302_D 1.278 0.95
142_Q 148_Q 1.268 0.95
192_A 211_V 1.262 0.94
161_L 181_V 1.259 0.94
340_P 343_K 1.249 0.94
112_R 116_K 1.243 0.94
221_N 225_L 1.242 0.94
42_M 191_A 1.24 0.94
146_Q 279_R 1.233 0.93
160_H 174_K 1.22 0.93
184_S 188_E 1.217 0.93
42_M 188_E 1.206 0.92
124_T 161_L 1.203 0.92
131_L 144_P 1.198 0.92
257_S 286_V 1.193 0.92
128_L 149_I 1.188 0.92
252_I 291_I 1.183 0.91
250_H 291_I 1.18 0.91
120_A 308_L 1.175 0.91
253_S 302_D 1.174 0.91
188_E 253_S 1.17 0.91
114_G 159_Q 1.17 0.91
228_R 246_D 1.168 0.91
69_L 195_L 1.166 0.91
107_V 172_L 1.166 0.91
69_L 191_A 1.163 0.91
109_T 297_E 1.158 0.90
206_R 218_R 1.157 0.90
149_I 153_K 1.154 0.90
48_A 199_M 1.154 0.90
323_A 341_Q 1.144 0.90
75_L 102_A 1.137 0.89
71_A 75_L 1.136 0.89
129_V 182_D 1.134 0.89
338_M 342_F 1.13 0.89
78_T 184_S 1.129 0.89
41_T 69_L 1.126 0.89
127_P 155_K 1.125 0.89
308_L 313_A 1.119 0.88
43_G 79_Y 1.117 0.88
196_A 216_N 1.112 0.88
91_S 94_L 1.107 0.87
151_A 154_A 1.102 0.87
187_G 214_A 1.102 0.87
99_V 104_A 1.098 0.87
38_E 47_R 1.097 0.87
184_S 214_A 1.094 0.87
62_K 65_I 1.087 0.86
113_I 301_W 1.084 0.86
263_E 268_R 1.082 0.86
132_W 269_L 1.079 0.86
182_D 260_N 1.074 0.85
143_I 269_L 1.073 0.85
164_I 169_Q 1.071 0.85
235_T 257_S 1.066 0.85
305_L 317_V 1.066 0.85
261_Y 268_R 1.064 0.84
206_R 221_N 1.064 0.84
210_S 253_S 1.063 0.84
270_S 306_M 1.063 0.84
44_T 212_G 1.063 0.84
88_F 181_V 1.061 0.84
258_Y 287_S 1.052 0.84
107_V 111_L 1.045 0.83
43_G 77_S 1.039 0.82
214_A 302_D 1.033 0.82
88_F 107_V 1.03 0.82
68_Q 102_A 1.03 0.82
299_D 303_T 1.028 0.82
190_Y 194_H 1.028 0.82
288_V 306_M 1.025 0.81
255_S 271_H 1.023 0.81
122_D 158_L 1.023 0.81
128_L 273_I 1.018 0.81
101_E 168_H 1.017 0.81
121_M 186_V 1.015 0.80
99_V 163_V 1.013 0.80
192_A 216_N 1.006 0.80
83_S 86_M 1.005 0.80
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2o18A40.94021000.098Contact Map0.771
4ig1A10.90881000.111Contact Map0.751
1vrmA10.90881000.135Contact Map0.793
2o34A20.64961000.681Contact Map0.543
3zbiC140.136813.60.972Contact Map0
4gveA10.376112.90.972Contact Map0.124
2jyaA10.284911.60.972Contact Map0.231
2kdkA10.1168100.973Contact Map0.699
2vlgA40.10549.70.973Contact Map0.731
2bfdB10.1889.10.974Contact Map0
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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