OPENSEQ.org - TUSE - ECOLI GREMLIN

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TUSE - Sulfurtransferase TusE

UniProt:	P0AB18	Sequence MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHWEVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGLPKPVKCI	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12876
Length:	109 (109)
Sequences:	437
Seq/Len:	4.01

TUSE
Paralog alert: 0.03 [within 20: 0.01] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
19_E	22_Q	4.177	1.00
85_Y	109_I	2.722	1.00
39_L	47_V	2.697	1.00
28_A	99_I	2.348	1.00
25_E	48_R	2.25	1.00
24_S	27_L	2.035	1.00
26_P	29_V	1.999	1.00
3_I	8_E	1.934	1.00
4_F	27_L	1.929	1.00
35_E	98_K	1.889	1.00
82_R	86_R	1.716	0.99
30_V	33_E	1.664	0.99
35_E	99_I	1.653	0.99
50_V	96_A	1.543	0.98
31_I	99_I	1.527	0.98
103_P	108_C	1.484	0.97
31_I	34_N	1.476	0.97
63_I	91_G	1.447	0.97
37_I	99_I	1.41	0.96
82_R	85_Y	1.398	0.96
91_G	97_T	1.294	0.93
28_A	44_W	1.268	0.92
104_K	108_C	1.245	0.91
43_H	87_L	1.241	0.91
82_R	107_K	1.229	0.90
49_F	69_A	1.224	0.90
40_S	43_H	1.19	0.88
105_P	108_C	1.19	0.88
5_E	26_P	1.181	0.88
10_E	22_Q	1.175	0.88
66_L	93_A	1.142	0.85
46_V	70_M	1.141	0.85
97_T	106_V	1.139	0.85
106_V	109_I	1.134	0.85
12_D	66_L	1.118	0.84
104_K	109_I	1.116	0.84
85_Y	91_G	1.105	0.83
71_A	76_E	1.102	0.83
44_W	47_V	1.098	0.82
45_E	79_G	1.081	0.81
16_Y	97_T	1.073	0.80
11_T	34_N	1.072	0.80
14_E	90_K	1.067	0.80
59_T	97_T	1.066	0.80
11_T	31_I	1.063	0.79
79_G	83_Y	1.047	0.78
37_I	98_K	1.026	0.76
4_F	30_V	1.024	0.76

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
1yx3A	1	1	100	-0.013	Contact Map	0.578
1sauA	1	0.9908	100	0.035	Contact Map	0.545
1ji8A	1	0.9358	100	0.125	Contact Map	0.374
3or1C	2	0.9358	100	0.132	Contact Map	0.501
3k2zA	2	0.633	60.2	0.91	Contact Map	0.708
1jhfA	2	0.6422	31.9	0.925	Contact Map	0.798
3eyiA	2	0.5872	23.2	0.929	Contact Map	0.545
4grhA	1	0.9266	21.6	0.931	Contact Map	0.159
1k0eA	2	0.9266	19.9	0.931	Contact Map	0.274
2vs7A	3	0.8257	17	0.933	Contact Map	0.332

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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