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OPENSEQ.org
SYT - Threonine--tRNA ligase
UniProt: P0A8M3 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11001
Length: 642 (634)
Sequences: 1807
Seq/Len: 2.85
SYT
Paralog alert: 0.06 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
499_G 505_K 5.203 1.00
551_Q 598_D 3.587 1.00
557_E 604_G 3.578 1.00
394_R 443_V 3.576 1.00
123_R 126_E 3.115 1.00
41_N 53_N 2.869 1.00
131_N 189_N 2.844 1.00
138_K 169_K 2.77 1.00
85_Q 123_R 2.767 1.00
442_A 446_E 2.758 1.00
293_V 340_I 2.742 1.00
559_T 569_V 2.623 1.00
394_R 447_E 2.621 1.00
301_R 305_E 2.576 1.00
252_D 344_G 2.481 1.00
75_C 222_A 2.471 1.00
137_K 174_F 2.471 1.00
491_S 506_V 2.443 1.00
499_G 503_E 2.437 1.00
561_K 622_N 2.395 1.00
255_H 267_H 2.394 1.00
107_D 155_Y 2.392 1.00
146_E 149_A 2.32 1.00
494_S 506_V 2.288 1.00
598_D 602_E 2.281 1.00
78_L 193_C 2.277 1.00
20_P 38_G 2.229 1.00
145_R 161_D 2.227 1.00
39_R 58_S 2.224 1.00
226_K 230_N 2.224 1.00
83_I 106_V 2.221 1.00
526_T 533_F 2.198 1.00
107_D 213_K 2.197 1.00
423_R 427_R 2.188 1.00
501_D 505_K 2.169 1.00
557_E 561_K 2.114 1.00
39_R 66_E 2.104 1.00
540_V 570_K 2.064 1.00
442_A 454_Y 2.051 1.00
78_L 127_L 2.046 1.00
85_Q 130_K 2.028 1.00
40_V 55_A 2.009 1.00
146_E 150_N 1.964 0.99
197_K 233_L 1.954 0.99
74_S 134_V 1.946 0.99
430_S 433_M 1.944 0.99
284_K 406_D 1.941 0.99
78_L 128_A 1.909 0.99
439_A 443_V 1.908 0.99
252_D 267_H 1.895 0.99
559_T 571_A 1.885 0.99
299_M 336_G 1.884 0.99
74_S 187_V 1.88 0.99
135_I 174_F 1.872 0.99
269_D 588_R 1.87 0.99
174_F 179_V 1.857 0.99
35_C 59_I 1.832 0.99
79_L 120_L 1.828 0.99
14_Y 18_V 1.827 0.99
547_I 578_I 1.826 0.99
302_V 305_E 1.819 0.99
284_K 288_Y 1.819 0.99
81_H 175_H 1.806 0.99
545_M 578_I 1.806 0.99
3_V 13_H 1.805 0.99
44_L 60_I 1.802 0.99
558_L 621_V 1.796 0.99
553_E 556_N 1.779 0.99
439_A 442_A 1.778 0.99
224_A 228_A 1.767 0.99
550_S 598_D 1.759 0.99
626_E 630_Q 1.752 0.99
72_R 221_T 1.751 0.99
145_R 157_V 1.741 0.98
108_L 112_L 1.724 0.98
428_I 488_S 1.722 0.98
574_R 584_E 1.717 0.98
398_N 447_E 1.713 0.98
446_E 452_F 1.71 0.98
623_E 626_E 1.688 0.98
269_D 540_V 1.672 0.98
225_D 228_A 1.664 0.98
24_A 27_I 1.663 0.98
164_I 170_P 1.663 0.98
199_M 236_L 1.656 0.98
237_E 240_A 1.656 0.98
544_I 559_T 1.64 0.98
594_L 606_V 1.639 0.98
84_K 178_Y 1.633 0.97
338_V 509_M 1.632 0.97
567_I 632_I 1.629 0.97
155_Y 213_K 1.628 0.97
343_Q 346_K 1.612 0.97
149_A 161_D 1.59 0.97
427_R 431_D 1.588 0.97
280_F 410_T 1.586 0.97
390_E 436_R 1.576 0.97
260_A 263_M 1.57 0.97
85_Q 126_E 1.567 0.97
3_V 11_Q 1.55 0.96
284_K 287_E 1.543 0.96
455_Q 458_E 1.532 0.96
445_L 466_I 1.525 0.96
556_N 571_A 1.507 0.95
22_D 25_L 1.502 0.95
297_F 362_H 1.5 0.95
469_T 477_A 1.499 0.95
532_F 589_R 1.498 0.95
545_M 593_M 1.498 0.95
417_V 477_A 1.491 0.95
121_E 197_K 1.487 0.95
81_H 132_Y 1.484 0.95
556_N 559_T 1.476 0.95
68_L 72_R 1.475 0.95
401_I 468_F 1.472 0.95
599_K 602_E 1.465 0.94
142_H 146_E 1.462 0.94
37_A 70_I 1.461 0.94
595_V 607_A 1.461 0.94
90_T 108_L 1.454 0.94
71_I 224_A 1.449 0.94
84_K 89_H 1.443 0.94
402_R 448_N 1.441 0.94
35_C 47_A 1.439 0.94
231_A 234_Q 1.429 0.93
548_T 551_Q 1.429 0.93
188_P 192_F 1.427 0.93
202_A 214_M 1.424 0.93
269_D 570_K 1.411 0.93
562_L 625_I 1.406 0.93
273_I 522_I 1.405 0.93
131_N 191_R 1.405 0.93
68_L 71_I 1.403 0.93
232_Y 236_L 1.392 0.92
140_S 143_E 1.377 0.92
354_R 396_E 1.368 0.91
561_K 604_G 1.365 0.91
586_T 593_M 1.365 0.91
559_T 563_S 1.364 0.91
30_G 33_K 1.364 0.91
30_G 34_A 1.363 0.91
21_M 25_L 1.362 0.91
133_D 189_N 1.356 0.91
45_V 49_D 1.354 0.91
620_D 623_E 1.348 0.90
49_D 136_K 1.348 0.90
85_Q 177_E 1.346 0.90
422_T 442_A 1.345 0.90
148_F 172_L 1.342 0.90
535_T 631_E 1.34 0.90
544_I 555_V 1.334 0.90
62_A 224_A 1.321 0.89
23_V 57_L 1.314 0.89
62_A 68_L 1.311 0.89
141_W 165_A 1.309 0.89
39_R 44_L 1.307 0.89
622_N 626_E 1.303 0.88
38_G 59_I 1.3 0.88
402_R 406_D 1.298 0.88
41_N 55_A 1.297 0.88
422_T 454_Y 1.295 0.88
100_N 235_R 1.29 0.88
266_W 522_I 1.276 0.87
542_V 628_L 1.276 0.87
393_I 485_L 1.274 0.87
619_M 627_K 1.274 0.87
344_G 350_D 1.273 0.87
19_S 22_D 1.271 0.87
346_K 353_L 1.26 0.86
94_I 207_R 1.259 0.86
280_F 406_D 1.258 0.86
286_K 563_S 1.255 0.86
14_Y 26_D 1.25 0.85
554_Y 596_C 1.249 0.85
346_K 350_D 1.246 0.85
366_P 369_S 1.246 0.85
172_L 179_V 1.241 0.85
567_I 628_L 1.24 0.85
247_I 251_L 1.24 0.85
20_P 45_V 1.233 0.84
112_L 218_I 1.229 0.84
397_V 466_I 1.229 0.84
67_G 224_A 1.226 0.84
252_D 255_H 1.225 0.84
435_D 438_E 1.224 0.84
630_Q 634_S 1.222 0.84
293_V 337_H 1.22 0.83
91_K 107_D 1.22 0.83
543_V 585_H 1.218 0.83
246_K 250_Q 1.217 0.83
233_L 236_L 1.215 0.83
27_I 31_L 1.214 0.83
585_H 593_M 1.209 0.83
312_N 426_K 1.209 0.83
58_S 64_D 1.208 0.83
94_I 159_I 1.206 0.82
555_V 571_A 1.204 0.82
560_Q 564_N 1.202 0.82
12_R 26_D 1.202 0.82
556_N 573_L 1.202 0.82
338_V 387_F 1.2 0.82
18_V 22_D 1.199 0.82
147_T 179_V 1.196 0.82
5_T 54_D 1.193 0.82
155_Y 212_N 1.191 0.81
440_D 485_L 1.188 0.81
278_E 294_K 1.183 0.81
305_E 311_D 1.183 0.81
199_M 221_T 1.183 0.81
625_I 629_Q 1.183 0.81
24_A 28_G 1.181 0.81
134_V 187_V 1.178 0.80
73_H 96_P 1.173 0.80
24_A 59_I 1.172 0.80
376_V 379_F 1.17 0.80
544_I 558_L 1.167 0.80
69_E 97_V 1.165 0.79
272_T 568_R 1.165 0.79
276_E 279_V 1.165 0.79
241_K 530_A 1.164 0.79
277_L 515_L 1.164 0.79
62_A 71_I 1.156 0.79
122_K 125_H 1.156 0.79
288_Y 406_D 1.153 0.78
535_T 632_I 1.149 0.78
73_H 77_H 1.148 0.78
40_V 49_D 1.144 0.78
143_E 146_E 1.142 0.77
137_K 143_E 1.141 0.77
599_K 603_S 1.139 0.77
287_E 564_N 1.138 0.77
281_V 285_L 1.134 0.77
158_S 207_R 1.131 0.76
386_I 400_C 1.129 0.76
227_K 230_N 1.123 0.76
288_Y 403_L 1.122 0.76
321_S 324_N 1.121 0.76
443_V 447_E 1.121 0.76
234_Q 237_E 1.12 0.75
90_T 106_V 1.116 0.75
15_D 18_V 1.115 0.75
498_V 504_R 1.113 0.75
209_D 212_N 1.111 0.75
113_T 117_V 1.106 0.74
552_S 573_L 1.105 0.74
260_A 299_M 1.105 0.74
134_V 175_H 1.102 0.74
535_T 628_L 1.102 0.74
203_G 214_M 1.1 0.74
281_V 407_M 1.098 0.73
604_G 622_N 1.097 0.73
228_A 231_A 1.096 0.73
310_W 328_C 1.096 0.73
547_I 579_G 1.093 0.73
92_M 106_V 1.093 0.73
542_V 567_I 1.092 0.73
28_G 34_A 1.092 0.73
86_L 120_L 1.085 0.72
8_D 27_I 1.084 0.72
360_S 378_G 1.083 0.72
379_F 517_S 1.083 0.72
549_D 552_S 1.082 0.72
133_D 191_R 1.075 0.71
98_I 103_Y 1.074 0.71
94_I 98_I 1.074 0.71
76_A 183_R 1.072 0.71
182_C 186_H 1.072 0.71
24_A 31_L 1.071 0.71
592_Y 631_E 1.07 0.71
274_F 294_K 1.069 0.71
395_D 399_G 1.069 0.71
556_N 560_Q 1.068 0.70
433_M 488_S 1.068 0.70
546_N 555_V 1.068 0.70
273_I 276_E 1.068 0.70
561_K 565_A 1.066 0.70
5_T 11_Q 1.065 0.70
139_V 172_L 1.064 0.70
25_L 29_P 1.062 0.70
586_T 589_R 1.061 0.70
391_E 500_E 1.06 0.70
58_S 66_E 1.059 0.70
132_Y 177_E 1.058 0.69
40_V 57_L 1.055 0.69
470_L 480_C 1.055 0.69
49_D 171_G 1.055 0.69
285_L 356_A 1.054 0.69
565_A 629_Q 1.052 0.69
561_K 621_V 1.052 0.69
274_F 278_E 1.051 0.69
391_E 436_R 1.045 0.68
275_R 279_V 1.042 0.68
98_I 207_R 1.041 0.68
145_R 149_A 1.041 0.68
238_E 241_K 1.037 0.67
553_E 557_E 1.036 0.67
424_P 459_G 1.036 0.67
306_K 493_L 1.036 0.67
561_K 564_N 1.035 0.67
561_K 625_I 1.034 0.67
532_F 611_R 1.034 0.67
629_Q 633_R 1.034 0.67
301_R 310_W 1.034 0.67
285_L 358_F 1.033 0.67
417_V 453_E 1.031 0.67
496_S 504_R 1.03 0.66
28_G 31_L 1.029 0.66
77_H 96_P 1.029 0.66
51_I 55_A 1.028 0.66
279_V 283_S 1.025 0.66
281_V 358_F 1.025 0.66
255_H 343_Q 1.023 0.66
263_M 378_G 1.022 0.66
557_E 560_Q 1.02 0.65
84_K 153_E 1.016 0.65
41_N 56_Q 1.015 0.65
235_R 474_L 1.014 0.65
627_K 631_E 1.014 0.65
84_K 90_T 1.013 0.65
472_D 479_Q 1.012 0.64
21_M 32_A 1.009 0.64
237_E 241_K 1.008 0.64
126_E 130_K 1.007 0.64
92_M 153_E 1.006 0.64
289_Q 563_S 1.005 0.64
415_K 471_Y 1.005 0.64
29_P 33_K 1.005 0.64
605_K 620_D 1.005 0.64
4_I 23_V 1.003 0.63
198_L 218_I 1.002 0.63
432_E 435_D 1.002 0.63
610_T 613_G 1 0.63
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1qf6A10.99841000.343Contact Map0.726
1nyrA20.98911000.374Contact Map0.78
4hwtA20.62621000.661Contact Map0.821
3uh0A10.66821000.662Contact Map0.67
1evlA40.62461000.663Contact Map0.806
3a32A10.63081000.683Contact Map0.566
3w3sA10.71651000.711Contact Map0.206
2zt5A10.7151000.715Contact Map0.512
1atiA20.58881000.724Contact Map0.497
3qneA10.69311000.727Contact Map0.329
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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