May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
TRMB - tRNA (guanine-N(7)-)-methyltransferase
UniProt: P0A8I5 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11779
Length: 239 (223)
Sequences: 1481
Seq/Len: 6.64
TRMB
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
136_R 170_Q 4.355 1.00
35_E 39_E 3.512 1.00
83_K 110_E 3.439 1.00
199_K 236_E 3.437 1.00
85_R 90_F 2.994 1.00
182_E 186_E 2.89 1.00
132_D 168_K 2.838 1.00
138_V 165_V 2.65 1.00
132_D 167_S 2.585 1.00
169_L 173_G 2.422 1.00
78_L 92_G 2.357 1.00
107_A 115_L 2.299 1.00
62_R 89_D 2.282 1.00
140_L 157_V 2.156 1.00
140_L 143_P 2.115 1.00
42_W 46_G 2.064 1.00
140_L 175_F 2.014 1.00
67_T 88_Q 1.978 1.00
72_F 92_G 1.918 1.00
55_D 58_A 1.885 1.00
177_M 188_M 1.862 1.00
163_E 195_I 1.855 1.00
151_H 154_R 1.844 1.00
166_K 197_G 1.812 1.00
57_P 63_E 1.804 1.00
187_H 191_V 1.76 1.00
67_T 139_Q 1.745 1.00
66_V 89_D 1.741 1.00
59_L 116_R 1.717 1.00
36_H 40_N 1.708 1.00
193_S 235_F 1.651 1.00
80_A 84_D 1.633 1.00
137_M 174_V 1.622 1.00
133_N 170_Q 1.585 1.00
56_F 66_V 1.563 0.99
152_N 183_P 1.559 0.99
195_I 198_Y 1.538 0.99
67_T 137_M 1.537 0.99
62_R 66_V 1.537 0.99
64_A 89_D 1.466 0.99
65_P 136_R 1.459 0.99
135_L 138_V 1.451 0.99
159_V 191_V 1.445 0.99
140_L 156_I 1.418 0.99
176_H 232_D 1.415 0.99
82_A 112_L 1.408 0.99
182_E 229_G 1.393 0.99
146_W 151_H 1.359 0.98
70_I 122_A 1.355 0.98
219_F 222_R 1.312 0.98
60_F 89_D 1.311 0.98
79_V 112_L 1.311 0.98
199_K 238_V 1.306 0.98
80_A 83_K 1.294 0.97
41_Y 108_H 1.259 0.97
92_G 103_C 1.25 0.97
41_Y 44_V 1.239 0.96
138_V 169_L 1.204 0.95
77_S 139_Q 1.192 0.95
68_L 135_L 1.19 0.95
135_L 168_K 1.185 0.95
83_K 112_L 1.177 0.95
179_T 185_A 1.174 0.95
223_G 228_H 1.172 0.94
216_V 221_Q 1.171 0.94
60_F 91_L 1.166 0.94
85_R 88_Q 1.16 0.94
159_V 194_S 1.157 0.94
76_A 221_Q 1.155 0.94
127_H 164_L 1.152 0.94
166_K 195_I 1.131 0.93
25_R 30_L 1.127 0.93
66_V 136_R 1.125 0.93
144_D 148_K 1.12 0.93
94_E 122_A 1.114 0.92
42_W 48_E 1.11 0.92
200_N 207_Y 1.106 0.92
61_G 64_A 1.102 0.92
130_I 134_S 1.09 0.91
142_F 217_T 1.081 0.91
64_A 87_E 1.075 0.90
146_W 154_R 1.071 0.90
131_P 134_S 1.066 0.90
183_P 186_E 1.062 0.90
140_L 165_V 1.056 0.89
97_S 119_C 1.05 0.89
70_I 138_V 1.049 0.89
184_Y 188_M 1.047 0.89
166_K 198_Y 1.046 0.89
65_P 89_D 1.037 0.88
33_G 36_H 1.025 0.87
49_F 128_K 1.021 0.87
32_K 36_H 1.014 0.87
133_N 237_R 1.012 0.86
152_N 187_H 1.012 0.86
177_M 184_Y 1.01 0.86
221_Q 225_R 1.006 0.86
79_V 103_C 1.006 0.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ckkA10.90791000.582Contact Map0.743
3dxyA10.87871000.585Contact Map0.837
2fcaA20.88281000.613Contact Map0.777
2vdvE20.89541000.624Contact Map0.716
1yzhA20.87451000.646Contact Map0.801
3p2eA20.790899.50.799Contact Map0.653
1o54A10.916399.40.811Contact Map0.592
3mb5A10.920599.40.812Contact Map0.602
1i9gA10.924799.40.814Contact Map0.641
2pwyA20.912199.40.817Contact Map0.641
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.0382 seconds.