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OPENSEQ.org

TRMD - tRNA (guanine-N(1)-)-methyltransferase
UniProt: P0A873 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11023
Length: 255 (243)
Sequences: 1623
Seq/Len: 6.68

TRMD
Paralog alert: 0.00 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
200_N 203_E 4.282 1.00
186_V 191_E 4.005 1.00
211_Q 214_G 3.811 1.00
94_L 99_V 3.619 1.00
120_E 124_Q 3.516 1.00
87_L 110_L 3.097 1.00
203_E 206_R 2.97 1.00
72_H 188_E 2.967 1.00
41_F 73_A 2.934 1.00
65_Q 69_D 2.873 1.00
133_I 147_T 2.826 1.00
6_I 38_P 2.705 1.00
37_S 40_D 2.665 1.00
43_H 69_D 2.552 1.00
136_Y 147_T 2.54 1.00
94_L 148_L 2.304 1.00
143_L 147_T 2.275 1.00
202_A 205_R 2.252 1.00
31_L 153_S 2.245 1.00
68_R 187_L 2.185 1.00
148_L 152_V 2.167 1.00
14_R 18_D 2.143 1.00
133_I 136_Y 2.132 1.00
186_V 189_G 2.104 1.00
98_G 131_W 2.055 1.00
5_I 149_I 2.013 1.00
98_G 129_E 2.013 1.00
101_E 129_E 2.007 1.00
125_T 190_M 2.001 1.00
209_L 233_Q 1.988 1.00
94_L 151_S 1.956 1.00
4_G 36_W 1.891 1.00
191_E 194_P 1.873 1.00
110_L 148_L 1.846 1.00
98_G 101_E 1.795 1.00
54_Y 185_E 1.761 1.00
68_R 126_E 1.751 1.00
68_R 188_E 1.751 1.00
44_D 48_T 1.746 1.00
139_S 150_D 1.741 1.00
214_G 218_L 1.722 1.00
83_K 129_E 1.709 1.00
48_T 63_M 1.67 1.00
166_A 169_D 1.641 1.00
83_K 105_N 1.627 1.00
23_G 27_K 1.621 1.00
215_R 219_R 1.609 1.00
124_Q 190_M 1.601 1.00
92_R 129_E 1.575 1.00
149_I 153_S 1.56 0.99
96_Q 155_F 1.549 0.99
68_R 72_H 1.519 0.99
4_G 34_Q 1.51 0.99
210_K 239_E 1.5 0.99
13_F 146_M 1.488 0.99
160_L 166_A 1.48 0.99
92_R 98_G 1.478 0.99
99_V 151_S 1.471 0.99
67_L 111_V 1.456 0.99
87_L 148_L 1.452 0.99
130_E 219_R 1.45 0.99
86_Y 111_V 1.43 0.99
52_R 183_R 1.429 0.99
62_M 118_I 1.427 0.99
11_E 19_Y 1.42 0.99
71_I 126_E 1.415 0.99
44_D 63_M 1.391 0.99
2_W 32_S 1.387 0.98
210_K 243_E 1.384 0.98
202_A 206_R 1.383 0.98
90_Q 215_R 1.37 0.98
4_G 107_K 1.369 0.98
11_E 14_R 1.344 0.98
11_E 37_S 1.339 0.98
54_Y 192_V 1.334 0.98
38_P 62_M 1.316 0.98
213_L 224_L 1.302 0.97
82_A 109_I 1.293 0.97
100_S 172_A 1.293 0.97
95_D 98_G 1.284 0.97
36_W 41_F 1.265 0.97
102_L 131_W 1.255 0.97
21_V 24_R 1.243 0.96
53_P 61_L 1.239 0.96
86_Y 118_I 1.238 0.96
171_F 223_L 1.236 0.96
205_R 232_E 1.204 0.95
185_E 191_E 1.196 0.95
2_W 34_Q 1.181 0.95
36_W 70_A 1.155 0.94
58_P 168_E 1.133 0.93
92_R 95_D 1.133 0.93
217_W 224_L 1.129 0.93
64_V 68_R 1.117 0.92
206_R 210_K 1.111 0.92
214_G 240_F 1.109 0.92
110_L 145_A 1.108 0.92
227_L 233_Q 1.096 0.92
30_L 156_I 1.093 0.91
16_I 146_M 1.089 0.91
112_C 145_A 1.081 0.91
4_G 109_I 1.069 0.90
3_I 149_I 1.062 0.90
97_A 100_S 1.055 0.89
18_D 26_V 1.048 0.89
61_L 180_H 1.045 0.89
164_A 167_T 1.041 0.89
209_L 213_L 1.037 0.88
133_I 144_P 1.03 0.88
144_P 147_T 1.025 0.87
3_I 31_L 1.018 0.87
67_L 118_I 1.012 0.87
177_D 180_H 1.01 0.86
209_L 227_L 1.004 0.86
155_F 172_A 1.003 0.86
57_G 208_R 1 0.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1ualA10.99611000.033Contact Map0.723
4mcdA10.96471000.051Contact Map0.726
4h3zA20.96861000.053Contact Map0.81
3ky7A10.96081000.057Contact Map0.695
1oy5A30.97251000.068Contact Map0.783
3knuA40.90981000.075Contact Map0.864
3iefA20.9021000.077Contact Map0.785
3quvA20.92161000.085Contact Map0.789
3aiaA20.537387.20.957Contact Map0.545
4fakA10.596185.90.958Contact Map0.533

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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