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OPENSEQ.org
TPIS - Triosephosphate isomerase
UniProt: P0A858 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11015
Length: 255 (249)
Sequences: 2248
Seq/Len: 9.03
TPIS
Paralog alert: 0.08 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
41_A 62_G 3.818 1.00
184_Q 226_D 3.812 1.00
135_E 176_K 3.481 1.00
139_T 186_V 2.938 1.00
138_K 141_E 2.914 1.00
13_N 71_S 2.484 1.00
44_E 78_T 2.464 1.00
106_D 145_R 2.367 1.00
155_G 158_A 2.337 1.00
238_K 241_A 2.295 1.00
148_D 193_H 2.285 1.00
116_L 121_L 2.24 1.00
133_E 138_K 2.132 1.00
113_F 125_L 2.093 1.00
143_C 186_V 2.076 1.00
181_A 224_Q 2.048 1.00
125_L 150_V 2.025 1.00
83_L 88_A 1.985 1.00
215_A 241_A 1.964 1.00
213_V 230_A 1.96 1.00
140_E 189_F 1.914 1.00
5_L 163_V 1.894 1.00
188_K 192_D 1.892 1.00
74_F 101_Y 1.885 1.00
131_E 135_E 1.87 1.00
133_E 142_V 1.864 1.00
14_G 18_M 1.826 1.00
131_E 176_K 1.814 1.00
66_V 80_A 1.789 1.00
60_M 89_Q 1.752 1.00
127_I 143_C 1.731 1.00
41_A 92_I 1.685 1.00
3_H 37_A 1.675 1.00
45_M 78_T 1.67 1.00
109_I 125_L 1.639 1.00
144_A 189_F 1.621 1.00
54_A 61_L 1.619 1.00
130_T 169_V 1.606 1.00
6_V 222_F 1.605 1.00
66_V 116_L 1.579 1.00
37_A 206_I 1.575 1.00
92_I 124_V 1.571 1.00
106_D 149_A 1.562 1.00
51_K 61_L 1.55 1.00
69_N 111_K 1.55 1.00
70_L 118_E 1.538 1.00
194_I 205_V 1.536 1.00
130_T 133_E 1.532 1.00
68_L 111_K 1.528 1.00
180_P 220_E 1.528 1.00
7_M 124_V 1.5 1.00
127_I 169_V 1.478 1.00
7_M 39_A 1.475 1.00
160_E 204_Q 1.462 1.00
91_I 116_L 1.462 1.00
213_V 222_F 1.458 1.00
65_N 102_H 1.449 1.00
5_L 39_A 1.443 0.99
195_A 202_A 1.437 0.99
2_R 228_D 1.405 0.99
218_A 245_I 1.399 0.99
140_E 185_A 1.391 0.99
38_V 246_V 1.381 0.99
63_A 83_L 1.369 0.99
183_A 224_Q 1.367 0.99
42_P 64_Q 1.366 0.99
183_A 221_L 1.363 0.99
144_A 148_D 1.356 0.99
105_S 108_L 1.355 0.99
117_K 162_A 1.328 0.99
67_D 102_H 1.326 0.99
158_A 161_G 1.324 0.99
19_V 46_Y 1.324 0.99
22_L 239_A 1.323 0.99
43_P 46_Y 1.311 0.99
70_L 119_Q 1.3 0.99
127_I 147_I 1.297 0.99
91_I 121_L 1.297 0.99
218_A 222_F 1.296 0.99
48_D 52_R 1.295 0.99
21_E 25_N 1.287 0.99
66_V 93_I 1.286 0.99
63_A 91_I 1.283 0.99
100_T 130_T 1.273 0.98
23_V 50_A 1.262 0.98
122_T 161_G 1.262 0.98
216_S 241_A 1.261 0.98
177_S 221_L 1.261 0.98
115_V 118_E 1.253 0.98
110_A 114_A 1.252 0.98
15_S 18_M 1.242 0.98
183_A 227_I 1.239 0.98
221_L 227_I 1.233 0.98
184_Q 225_P 1.232 0.98
133_E 145_R 1.23 0.98
41_A 124_V 1.212 0.98
90_Y 122_T 1.209 0.98
114_A 118_E 1.208 0.98
69_N 74_F 1.208 0.98
42_P 50_A 1.205 0.98
192_D 196_K 1.201 0.97
213_V 232_V 1.195 0.97
14_G 19_V 1.193 0.97
180_P 183_A 1.193 0.97
110_A 153_T 1.193 0.97
19_V 50_A 1.186 0.97
80_A 115_V 1.184 0.97
134_N 182_Q 1.174 0.97
25_N 240_D 1.159 0.97
68_L 108_L 1.154 0.97
181_A 185_A 1.15 0.96
68_L 102_H 1.149 0.96
89_Q 122_T 1.148 0.96
65_N 77_E 1.136 0.96
80_A 119_Q 1.132 0.96
182_Q 185_A 1.122 0.96
5_L 208_Q 1.113 0.96
15_S 46_Y 1.111 0.95
148_D 197_V 1.099 0.95
39_A 90_Y 1.093 0.95
195_A 203_E 1.092 0.95
141_E 144_A 1.084 0.95
67_D 115_V 1.077 0.94
24_S 53_E 1.074 0.94
181_A 220_E 1.074 0.94
4_P 222_F 1.071 0.94
77_E 102_H 1.049 0.93
66_V 91_I 1.048 0.93
142_V 169_V 1.047 0.93
191_R 207_I 1.046 0.93
134_N 185_A 1.041 0.93
189_F 193_H 1.041 0.93
130_T 142_V 1.036 0.93
26_L 59_I 1.036 0.93
132_A 136_A 1.028 0.92
100_T 169_V 1.027 0.92
65_N 112_K 1.017 0.92
54_A 59_I 1.006 0.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4k6aA211000.028Contact Map0.783
1aw2A80.99611000.029Contact Map0.775
3m9yA20.97651000.038Contact Map0.807
2j27A20.96861000.043Contact Map0.691
1yyaA20.97651000.043Contact Map0.745
3ta6A211000.043Contact Map0.777
1m6jA20.98821000.043Contact Map0.764
2btmA20.98431000.043Contact Map0.798
1o5xA20.96471000.045Contact Map0.814
2yc6A10.98821000.046Contact Map0.69
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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