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OPENSEQ.org
RECF - DNA replication and repair protein RecF
UniProt: P0A7H0 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10828
Length: 357 (356)
Sequences: 1418
Seq/Len: 3.98
RECF
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
241_D 248_A 4.936 1.00
10_D 62_R 3.727 1.00
3_L 43_I 3.517 1.00
162_N 247_T 3.322 1.00
46_L 72_G 3.301 1.00
178_D 238_F 3.273 1.00
182_I 231_A 2.771 1.00
161_R 247_T 2.725 1.00
196_S 256_L 2.685 1.00
8_I 14_I 2.641 1.00
3_L 72_G 2.502 1.00
21_L 328_F 2.497 1.00
112_M 298_L 2.478 1.00
150_A 187_Q 2.396 1.00
144_E 191_W 2.343 1.00
40_L 300_L 2.316 1.00
154_L 252_H 2.24 1.00
4_T 75_Q 2.217 1.00
15_E 62_R 2.159 1.00
14_I 17_A 2.125 1.00
104_K 107_E 2.101 1.00
81_T 98_D 2.052 1.00
158_L 181_L 2.047 1.00
175_R 238_F 2.008 1.00
136_L 256_L 2 1.00
265_V 269_L 1.981 1.00
178_D 234_L 1.944 1.00
28_L 39_V 1.935 1.00
117_I 275_K 1.906 1.00
265_V 277_L 1.885 1.00
43_I 328_F 1.849 1.00
233_V 249_H 1.841 1.00
207_C 276_L 1.838 1.00
160_Q 177_W 1.813 1.00
26_N 348_F 1.77 0.99
237_N 241_D 1.769 0.99
154_L 185_A 1.767 0.99
240_R 243_Q 1.763 0.99
47_G 114_M 1.751 0.99
276_L 312_R 1.699 0.99
157_L 184_L 1.693 0.99
232_E 236_R 1.676 0.99
154_L 181_L 1.665 0.99
181_L 185_A 1.658 0.99
178_D 241_D 1.618 0.99
198_G 292_E 1.595 0.99
185_A 251_P 1.573 0.98
182_I 230_Y 1.522 0.98
97_I 111_L 1.506 0.98
290_T 296_R 1.505 0.98
178_D 235_E 1.487 0.97
203_M 216_L 1.45 0.97
125_L 278_M 1.45 0.97
183_P 227_E 1.442 0.97
269_L 277_L 1.433 0.97
185_A 230_Y 1.421 0.96
214_F 273_Q 1.404 0.96
237_N 240_R 1.397 0.96
62_R 65_Q 1.391 0.96
17_A 39_V 1.376 0.95
5_R 18_D 1.371 0.95
131_Y 134_A 1.363 0.95
193_A 197_A 1.358 0.95
12_R 41_E 1.347 0.95
120_E 126_N 1.346 0.95
216_L 277_L 1.332 0.94
122_F 275_K 1.327 0.94
150_A 184_L 1.317 0.94
351_E 354_K 1.31 0.93
22_S 346_K 1.302 0.93
84_G 96_R 1.287 0.93
13_N 353_G 1.279 0.92
163_A 167_Q 1.275 0.92
194_E 198_G 1.274 0.92
123_T 127_G 1.267 0.92
244_L 248_A 1.267 0.92
183_P 187_Q 1.254 0.91
194_E 197_A 1.253 0.91
68_F 87_K 1.253 0.91
41_E 53_R 1.24 0.91
34_S 353_G 1.233 0.90
237_N 248_A 1.224 0.90
146_G 187_Q 1.217 0.90
313_R 333_S 1.216 0.90
74_L 112_M 1.209 0.89
239_E 242_R 1.194 0.88
175_R 235_E 1.194 0.88
9_R 16_T 1.192 0.88
115_Q 297_C 1.191 0.88
179_K 231_A 1.19 0.88
28_L 328_F 1.188 0.88
284_A 299_Y 1.186 0.88
350_V 355_I 1.181 0.88
149_T 153_N 1.18 0.88
189_S 193_A 1.171 0.87
181_L 247_T 1.169 0.87
117_I 301_I 1.161 0.87
198_G 291_R 1.156 0.86
102_G 108_L 1.147 0.86
199_I 203_M 1.144 0.85
71_H 84_G 1.143 0.85
27_F 331_A 1.143 0.85
59_R 268_T 1.14 0.85
154_L 230_Y 1.137 0.85
28_L 348_F 1.135 0.85
230_Y 234_L 1.134 0.85
227_E 231_A 1.124 0.84
240_R 244_L 1.124 0.84
14_I 316_L 1.119 0.84
140_C 147_F 1.119 0.84
34_S 350_V 1.119 0.84
147_F 151_W 1.116 0.83
233_V 236_R 1.111 0.83
122_F 307_E 1.111 0.83
4_T 80_E 1.11 0.83
53_R 60_V 1.107 0.83
142_H 285_Q 1.105 0.83
221_Q 257_R 1.101 0.82
275_K 307_E 1.1 0.82
258_I 277_L 1.097 0.82
271_R 312_R 1.092 0.82
350_V 353_G 1.086 0.81
143_N 288_F 1.084 0.81
38_S 301_I 1.084 0.81
5_R 73_R 1.082 0.81
219_S 257_R 1.081 0.81
17_A 355_I 1.08 0.81
12_R 53_R 1.08 0.81
351_E 356_T 1.079 0.81
143_N 289_L 1.078 0.80
7_L 16_T 1.078 0.80
283_L 316_L 1.076 0.80
205_D 208_K 1.074 0.80
67_A 86_T 1.072 0.80
26_N 328_F 1.071 0.80
73_R 80_E 1.069 0.80
217_T 262_G 1.068 0.80
186_E 229_E 1.059 0.79
83_I 108_L 1.056 0.78
153_N 184_L 1.055 0.78
217_T 259_R 1.053 0.78
126_N 271_R 1.053 0.78
180_E 183_P 1.051 0.78
44_Y 116_L 1.05 0.78
139_G 195_Y 1.048 0.78
117_I 279_C 1.042 0.77
117_I 304_F 1.041 0.77
191_W 194_E 1.038 0.77
161_R 178_D 1.037 0.77
43_I 72_G 1.037 0.77
124_L 135_F 1.029 0.76
19_L 355_I 1.029 0.76
204_A 208_K 1.029 0.76
286_G 299_Y 1.025 0.76
151_W 188_I 1.024 0.75
185_A 234_L 1.022 0.75
253_K 279_C 1.022 0.75
7_L 71_H 1.017 0.75
348_F 355_I 1.016 0.75
63_H 263_A 1.016 0.75
178_D 248_A 1.012 0.74
279_C 299_Y 1.004 0.73
105_V 289_L 1 0.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2o5vA10.9721000.447Contact Map0.644
4abyA40.8881000.53Contact Map0.465
3auyA20.9441000.614Contact Map0.596
3qktA40.86271000.622Contact Map0.588
4ad8A10.94121000.622Contact Map0.375
4i99A20.82071000.644Contact Map0.579
3zgxA20.9581000.677Contact Map0.374
3qf7A20.91041000.684Contact Map0.55
1w1wA40.931000.708Contact Map0.597
1e69A60.78991000.746Contact Map0.631
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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