OPENSEQ.org - PYRI - ECOLI GREMLIN

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PYRI - Aspartate carbamoyltransferase regulatory chain

UniProt:	P0A7F3	Sequence MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITIGLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPERIDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG10811
Length:	153 (149)
Sequences:	389
Seq/Len:	2.61

PYRI
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
13_K	88_N	2.041	1.00
26_G	57_D	1.97	0.99
64_T	70_Q	1.948	0.99
30_L	57_D	1.707	0.98
29_L	35_L	1.697	0.98
102_R	124_S	1.665	0.97
42_I	59_I	1.649	0.97
76_L	103_I	1.648	0.97
65_F	87_D	1.627	0.97
116_S	123_S	1.619	0.97
12_I	65_F	1.605	0.97
38_T	44_I	1.567	0.96
124_S	139_K	1.549	0.95
34_K	37_E	1.548	0.95
45_G	48_L	1.547	0.95
63_N	142_E	1.471	0.94
101_E	130_R	1.46	0.93
22_P	78_A	1.459	0.93
103_I	130_R	1.442	0.93
93_G	129_K	1.429	0.92
57_D	62_E	1.426	0.92
14_R	87_D	1.415	0.92
29_L	77_Y	1.4	0.91
11_A	45_G	1.395	0.91
107_L	136_L	1.39	0.91
9_V	92_V	1.376	0.90
29_L	59_I	1.375	0.90
34_K	59_I	1.345	0.89
87_D	92_V	1.344	0.89
25_I	45_G	1.317	0.87
24_Q	57_D	1.283	0.86
43_T	58_L	1.251	0.84
74_L	97_P	1.251	0.84
25_I	28_K	1.234	0.82
86_I	137_K	1.226	0.82
115_I	119_E	1.217	0.81
113_N	142_E	1.196	0.80
26_G	35_L	1.195	0.80
86_I	98_S	1.191	0.79
30_L	120_P	1.189	0.79
7_L	91_V	1.152	0.76
99_L	135_A	1.152	0.76
39_D	80_Q	1.151	0.76
101_E	104_D	1.149	0.76
104_D	124_S	1.146	0.75
71_V	97_P	1.132	0.74
43_T	143_K	1.129	0.74
62_E	82_T	1.124	0.73
7_L	62_E	1.124	0.73
126_A	139_K	1.123	0.73
13_K	89_Y	1.118	0.73
76_L	145_F	1.117	0.73
107_L	147_H	1.116	0.73
64_T	72_D	1.112	0.72
129_K	133_D	1.109	0.72
25_I	29_L	1.106	0.72
36_T	116_S	1.101	0.71
83_V	130_R	1.101	0.71
85_R	107_L	1.097	0.71
12_I	17_V	1.097	0.71
9_V	49_P	1.097	0.71
86_I	128_R	1.085	0.70
137_K	142_E	1.078	0.69
108_V	131_A	1.071	0.68
107_L	144_E	1.067	0.68
102_R	139_K	1.064	0.67
59_I	77_Y	1.055	0.66
108_V	117_H	1.055	0.66
104_D	122_S	1.045	0.65
7_L	48_L	1.045	0.65
26_G	30_L	1.041	0.65
11_A	48_L	1.03	0.64
146_S	149_V	1.026	0.63
57_D	82_T	1.026	0.63
81_A	121_V	1.025	0.63
65_F	89_Y	1.019	0.63
27_F	81_A	1.012	0.62
30_L	123_S	1.011	0.62
16_T	64_T	1.007	0.61
117_H	123_S	1.006	0.61

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
4fyyB	2	0.9935	100	0.017	Contact Map	0.389
2be7D	3	0.9935	100	0.033	Contact Map	0.44
1pg5B	1	0.9935	100	0.04	Contact Map	0.418
2ywwA	2	0.9739	100	0.054	Contact Map	0.439
2jrrA	1	0.3529	31.8	0.947	Contact Map	0.066
1dl6A	1	0.3137	16.4	0.953	Contact Map	0.033
2eluA	1	0.1765	15.5	0.954	Contact Map	0
1bboA	1	0.1242	13.6	0.955	Contact Map	0.714
2adrA	1	0.1307	12.3	0.956	Contact Map	0.739
1gh9A	1	0.2484	12.2	0.956	Contact Map	0.454

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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