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OPENSEQ.org

PIMT - Protein-L-isoaspartate O-methyltransferase
UniProt: P0A7A5 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10689
Length: 208 (202)
Sequences: 1908
Seq/Len: 9.45

PIMT
Paralog alert: 0.23 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
104_E 110_Q 2.76 1.00
156_T 160_T 2.572 1.00
135_G 161_Q 2.539 1.00
95_H 122_L 2.506 1.00
84_T 102_S 2.431 1.00
167_I 181_R 2.391 1.00
102_S 127_T 2.332 1.00
29_A 95_H 2.321 1.00
143_D 163_D 2.31 1.00
112_Q 116_R 2.244 1.00
136_W 142_F 2.204 1.00
110_Q 127_T 2.169 1.00
167_I 183_R 2.157 1.00
77_S 144_A 2.154 1.00
99_H 126_S 2.134 1.00
159_M 189_F 2.134 1.00
19_Q 69_E 2.126 1.00
184_R 189_F 2.099 1.00
147_V 170_L 2.094 1.00
128_R 136_W 1.977 1.00
110_Q 129_H 1.962 1.00
164_E 186_G 1.94 1.00
73_L 144_A 1.788 1.00
68_T 92_I 1.777 1.00
117_L 125_V 1.734 1.00
135_G 141_P 1.714 1.00
71_L 144_A 1.707 1.00
147_V 158_L 1.693 1.00
139_R 142_F 1.692 1.00
68_T 89_Q 1.689 1.00
107_K 129_H 1.685 1.00
70_L 194_V 1.633 1.00
181_R 192_D 1.625 1.00
13_L 18_I 1.625 1.00
34_K 119_N 1.62 1.00
107_K 111_W 1.609 1.00
141_P 161_Q 1.601 1.00
81_E 102_S 1.55 1.00
9_L 27_L 1.539 1.00
144_A 167_I 1.537 1.00
21_E 25_N 1.515 1.00
8_A 12_Q 1.508 1.00
27_L 92_I 1.493 1.00
117_L 127_T 1.488 1.00
78_R 139_R 1.473 1.00
54_Q 108_G 1.431 1.00
97_V 124_N 1.424 0.99
154_I 182_V 1.404 0.99
73_L 93_L 1.403 0.99
81_E 148_T 1.399 0.99
135_G 140_A 1.392 0.99
90_T 102_S 1.386 0.99
171_P 199_F 1.385 0.99
33_E 45_W 1.381 0.99
178_Y 193_T 1.341 0.99
116_R 120_L 1.334 0.99
140_A 161_Q 1.333 0.99
25_N 29_A 1.317 0.99
180_K 193_T 1.316 0.99
105_R 203_V 1.298 0.99
183_R 192_D 1.295 0.99
149_A 202_L 1.293 0.99
67_M 199_F 1.248 0.98
94_A 124_N 1.242 0.98
54_Q 106_I 1.238 0.98
164_E 184_R 1.236 0.98
168_L 182_V 1.234 0.98
20_D 72_E 1.229 0.98
63_M 197_V 1.229 0.98
90_T 127_T 1.223 0.98
101_C 139_R 1.21 0.98
14_R 28_A 1.2 0.98
67_M 146_I 1.187 0.97
73_L 97_V 1.175 0.97
180_K 191_I 1.173 0.97
185_R 188_E 1.158 0.97
30_V 95_H 1.152 0.97
164_E 185_R 1.138 0.97
47_N 62_Y 1.135 0.96
14_R 24_L 1.13 0.96
76_Q 97_V 1.123 0.96
181_R 194_V 1.12 0.96
90_T 125_V 1.119 0.96
145_I 162_L 1.105 0.96
78_R 99_H 1.1 0.96
34_K 120_L 1.098 0.95
153_E 156_T 1.094 0.95
66_R 195_E 1.093 0.95
114_R 118_K 1.077 0.95
9_L 12_Q 1.074 0.95
59_S 171_P 1.066 0.94
22_Q 75_P 1.064 0.94
89_Q 146_I 1.061 0.94
24_L 28_A 1.061 0.94
89_Q 148_T 1.059 0.94
36_V 50_L 1.042 0.94
30_V 88_Y 1.041 0.94
151_P 202_L 1.034 0.93
111_W 114_R 1.023 0.93
30_V 122_L 1.015 0.92
20_D 69_E 1.013 0.92
184_R 187_G 1.013 0.92
102_S 148_T 1.011 0.92
22_Q 96_L 1.007 0.92
14_R 21_E 1.002 0.92
56_Q 200_V 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1jg1A10.99521000.466Contact Map0.817
3lbfA411000.516Contact Map0.85
1r18A10.95191000.519Contact Map0.726
2pbfA20.96631000.521Contact Map0.763
1i1nA10.98081000.527Contact Map0.758
2yxeA20.99521000.528Contact Map0.881
1vbfA40.95671000.549Contact Map0.819
1dl5A20.9761000.562Contact Map0.843
1yb2A10.985699.90.603Contact Map0.373
3htxA20.995299.90.615Contact Map0.365

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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