OPENSEQ.org - ORN - ECOLI GREMLIN

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ORN - Oligoribonuclease

UniProt:	P0A784	Sequence MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGPTIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKSPICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQAMDDIRESVAELAYYREHFIKL	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12480
Length:	181 (178)
Sequences:	721
Seq/Len:	4.05

ORN
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
73_E	77_A	3.878	1.00
67_T	72_V	3.411	1.00
52_D	76_K	3.391	1.00
52_D	56_A	2.663	1.00
34_D	38_N	2.606	1.00
137_L	179_I	2.605	1.00
151_G	173_Y	2.595	1.00
46_I	80_M	2.562	1.00
26_I	75_V	2.447	1.00
109_I	131_Y	2.375	1.00
8_L	132_L	2.371	1.00
81_G	84_E	2.244	1.00
30_T	89_T	2.231	1.00
159_Q	162_D	2.062	1.00
31_L	168_V	2.042	1.00
47_A	74_R	1.991	1.00
13_L	25_I	1.929	1.00
64_R	68_A	1.924	1.00
59_D	63_V	1.899	1.00
24_R	82_D	1.855	1.00
141_A	178_F	1.849	1.00
84_E	88_A	1.806	1.00
31_L	165_R	1.777	0.99
60_D	64_R	1.741	0.99
54_Q	57_L	1.72	0.99
23_D	54_Q	1.696	0.99
87_L	91_E	1.683	0.99
87_L	122_E	1.656	0.99
21_E	118_K	1.636	0.99
169_A	172_A	1.636	0.99
93_L	123_L	1.596	0.99
98_P	126_Y	1.584	0.98
16_T	26_I	1.584	0.98
104_I	127_F	1.556	0.98
19_D	22_R	1.509	0.98
46_I	88_A	1.508	0.98
40_L	96_W	1.503	0.98
8_L	105_C	1.495	0.98
42_E	165_R	1.495	0.98
113_R	124_E	1.494	0.98
86_E	122_E	1.456	0.97
110_G	131_Y	1.443	0.97
90_L	94_K	1.438	0.97
162_D	166_E	1.43	0.97
139_E	142_R	1.425	0.96
31_L	39_I	1.395	0.96
60_D	63_V	1.371	0.95
80_M	88_A	1.355	0.95
83_R	86_E	1.342	0.95
24_R	119_Y	1.33	0.94
11_I	116_L	1.269	0.92
148_I	173_Y	1.255	0.92
63_V	72_V	1.24	0.91
10_W	14_E	1.215	0.90
65_T	166_E	1.215	0.90
28_I	89_T	1.207	0.89
139_E	143_R	1.203	0.89
178_F	181_L	1.192	0.89
65_T	69_S	1.186	0.88
80_M	85_A	1.156	0.87
44_P	92_F	1.154	0.86
41_A	92_F	1.145	0.86
91_E	95_Q	1.114	0.84
65_T	68_A	1.108	0.83
13_L	116_L	1.102	0.83
145_K	148_I	1.102	0.83
46_I	85_A	1.077	0.81
97_V	126_Y	1.065	0.80
41_A	44_P	1.063	0.80
110_G	119_Y	1.062	0.79
94_K	122_E	1.061	0.79
93_L	126_Y	1.055	0.79
48_V	85_A	1.041	0.78
41_A	95_Q	1.041	0.78
111_Q	114_R	1.037	0.77
20_P	119_Y	1.032	0.77
83_R	87_L	1.028	0.76
93_L	100_G	1.024	0.76
50_Q	82_D	1.022	0.76
13_L	92_F	1.018	0.75
62_N	72_V	1.007	0.74

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
2gbzA	1	1	100	0.636	Contact Map	0.783
3tr8A	2	1	100	0.647	Contact Map	0.812
2igiA	2	0.9945	100	0.661	Contact Map	0.789
3u3yB	2	0.9779	99.9	0.742	Contact Map	0.38
3mxmB	2	0.9779	99.9	0.748	Contact Map	0.346
1w0hA	1	0.9503	99.8	0.76	Contact Map	0.477
4hxhB	2	0.9503	99.8	0.767	Contact Map	0.552
2qxfA	1	0.9337	99.8	0.767	Contact Map	0.515
1zbuA	4	0.9503	99.8	0.772	Contact Map	0.566
2xriA	1	0.9448	99.8	0.773	Contact Map	0.593

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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