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OPENSEQ.org

NUSB - N utilization substance protein B
UniProt: P0A780 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10666
Length: 139 (133)
Sequences: 2491
Seq/Len: 18.73

NUSB
Paralog alert: 0.76 [within 20: 0.05] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
63_D 86_R 3.803 1.00
54_G 90_Y 3.499 1.00
89_L 130_A 2.715 1.00
88_A 107_A 2.605 1.00
58_N 61_Y 2.408 1.00
13_A 51_L 2.15 1.00
84_V 111_A 2.073 1.00
112_K 120_H 2.056 1.00
11_E 113_S 1.958 1.00
129_K 133_V 1.946 1.00
98_V 103_A 1.892 1.00
129_K 132_P 1.838 1.00
62_L 90_Y 1.782 1.00
99_P 102_V 1.73 1.00
70_L 77_L 1.729 1.00
47_Y 98_V 1.679 1.00
111_A 123_V 1.655 1.00
14_V 87_I 1.601 1.00
62_L 89_L 1.585 1.00
93_S 130_A 1.581 1.00
69_Y 130_A 1.554 1.00
70_L 122_F 1.543 1.00
15_Q 114_F 1.503 1.00
65_L 130_A 1.452 1.00
18_Y 22_L 1.441 1.00
16_A 48_F 1.44 1.00
79_Q 82_K 1.432 1.00
61_Y 94_K 1.423 1.00
58_N 94_K 1.418 1.00
14_V 83_A 1.417 1.00
105_N 109_E 1.406 0.99
69_Y 133_V 1.395 0.99
9_A 48_F 1.386 0.99
50_E 95_R 1.36 0.99
21_Q 86_R 1.356 0.99
19_S 23_S 1.343 0.99
13_A 52_L 1.331 0.99
84_V 107_A 1.317 0.99
92_L 103_A 1.293 0.99
51_L 87_I 1.281 0.99
108_I 120_H 1.279 0.99
88_A 103_A 1.277 0.99
104_I 131_A 1.276 0.99
66_M 89_L 1.275 0.99
49_R 53_A 1.272 0.99
50_E 90_Y 1.238 0.98
109_E 112_K 1.219 0.98
21_Q 82_K 1.217 0.98
67_K 74_L 1.206 0.98
73_L 76_E 1.205 0.98
100_Y 131_A 1.192 0.98
20_W 56_A 1.17 0.97
108_I 124_N 1.154 0.97
11_E 114_F 1.142 0.97
78_G 81_E 1.14 0.97
71_S 129_K 1.134 0.97
84_V 123_V 1.127 0.97
68_P 133_V 1.118 0.96
65_L 89_L 1.114 0.96
92_L 100_Y 1.088 0.96
9_A 13_A 1.082 0.96
12_C 34_F 1.074 0.95
90_Y 94_K 1.071 0.95
77_L 122_F 1.067 0.95
92_L 131_A 1.058 0.95
64_G 68_P 1.052 0.95
82_K 86_R 1.042 0.94
51_L 90_Y 1.026 0.94
46_L 50_E 1.016 0.93
81_E 119_S 1.016 0.93
50_E 57_T 1.012 0.93
106_E 109_E 1.007 0.93
117_E 120_H 1.006 0.93
16_A 34_F 1.004 0.93
91_E 107_A 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3d3bA111000.046Contact Map0.788
3r2dA20.99281000.07Contact Map0.849
1tzvA10.98561000.081Contact Map0.845
1eyvA211000.111Contact Map0.86
1sqgA10.9641000.215Contact Map0.83
1q8cA10.92811000.219Contact Map0.51
2yxlA10.98561000.256Contact Map0.662
1v54E20.597118.20.917Contact Map0.179
3hazA20.992810.40.926Contact Map0.193
3e2qA10.856110.30.926Contact Map0.099

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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