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OPENSEQ.org
HEM1 - Glutamyl-tRNA reductase
UniProt: P0A6X1 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10427
Length: 418 (418)
Sequences: 1259
Seq/Len: 3.01
HEM1
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
102_R 322_E 5.351 1.00
276_L 285_V 4.827 1.00
151_R 155_E 3.751 1.00
152_V 321_A 3.586 1.00
280_A 284_D 3.459 1.00
248_S 284_D 3.376 1.00
43_G 135_A 3.208 1.00
45_V 142_F 3.085 1.00
184_T 210_I 2.934 1.00
188_V 257_I 2.826 1.00
208_K 229_E 2.611 1.00
317_A 320_E 2.576 1.00
248_S 285_V 2.57 1.00
356_R 381_A 2.481 1.00
7_G 89_Y 2.448 1.00
184_T 208_K 2.316 1.00
235_D 238_E 2.297 1.00
151_R 328_E 2.235 1.00
14_P 87_S 2.222 1.00
151_R 327_Q 2.164 1.00
353_E 356_R 2.159 1.00
141_M 332_F 2.055 1.00
47_L 121_V 2.041 1.00
98_S 326_A 2.026 1.00
359_L 387_R 2.004 1.00
255_P 286_E 1.958 1.00
248_S 280_A 1.911 0.99
370_G 373_A 1.887 0.99
276_L 295_A 1.879 0.99
185_V 206_V 1.872 0.99
328_E 331_E 1.849 0.99
100_L 121_V 1.848 0.99
359_L 362_K 1.821 0.99
332_F 335_W 1.818 0.99
174_R 204_H 1.814 0.99
216_R 232_A 1.791 0.99
19_E 283_R 1.762 0.99
260_G 263_E 1.738 0.99
250_T 284_D 1.738 0.99
146_F 149_A 1.72 0.99
105_S 152_V 1.718 0.99
128_S 133_M 1.708 0.98
220_Q 224_D 1.699 0.98
349_R 386_N 1.699 0.98
97_V 329_T 1.671 0.98
262_V 265_A 1.662 0.98
182_T 207_Q 1.651 0.98
14_P 17_L 1.637 0.98
352_A 388_L 1.597 0.97
32_L 133_M 1.595 0.97
349_R 393_T 1.593 0.97
359_L 383_K 1.592 0.97
188_V 285_V 1.581 0.97
216_R 220_Q 1.577 0.97
258_G 261_M 1.573 0.97
129_Q 139_E 1.564 0.97
116_Q 196_L 1.553 0.97
216_R 230_V 1.542 0.96
17_L 87_S 1.53 0.96
56_Y 103_V 1.524 0.96
259_K 263_E 1.513 0.96
5_A 56_Y 1.497 0.96
157_D 160_A 1.497 0.96
259_K 288_E 1.493 0.96
312_A 315_K 1.492 0.96
212_A 231_I 1.49 0.95
56_Y 142_F 1.483 0.95
363_A 376_I 1.48 0.95
190_A 213_N 1.475 0.95
285_V 289_V 1.47 0.95
199_R 225_E 1.451 0.95
407_R 414_S 1.442 0.94
105_S 321_A 1.438 0.94
102_R 108_D 1.43 0.94
118_L 149_A 1.427 0.94
94_N 333_M 1.42 0.94
23_F 31_A 1.4 0.93
18_R 301_D 1.392 0.93
71_R 75_D 1.387 0.93
356_R 382_W 1.384 0.93
254_L 258_G 1.378 0.92
169_A 279_I 1.378 0.92
57_L 69_L 1.365 0.92
186_L 212_A 1.364 0.92
53_T 106_G 1.362 0.92
8_I 21_V 1.361 0.92
9_N 108_D 1.356 0.92
239_R 242_E 1.356 0.92
97_V 141_M 1.347 0.91
45_V 124_A 1.341 0.91
162_A 167_F 1.339 0.91
363_A 380_L 1.329 0.90
162_A 168_A 1.322 0.90
259_K 291_K 1.318 0.90
56_Y 106_G 1.318 0.90
254_L 260_G 1.307 0.89
165_V 279_I 1.306 0.89
148_V 325_V 1.299 0.89
255_P 287_P 1.295 0.89
351_Q 391_A 1.295 0.89
298_Y 302_D 1.292 0.89
246_I 276_L 1.291 0.89
379_D 383_K 1.288 0.89
89_Y 108_D 1.287 0.88
262_V 289_V 1.28 0.88
364_L 374_Q 1.274 0.88
31_A 46_V 1.264 0.87
97_V 333_M 1.234 0.85
153_R 363_A 1.232 0.85
198_A 226_V 1.227 0.85
285_V 297_L 1.221 0.85
210_I 243_A 1.218 0.84
176_I 296_Y 1.214 0.84
356_R 378_Q 1.205 0.84
140_R 146_F 1.204 0.84
71_R 81_E 1.203 0.83
165_V 193_T 1.202 0.83
210_I 231_I 1.2 0.83
80_N 83_D 1.197 0.83
353_E 357_D 1.192 0.83
262_V 276_L 1.189 0.82
31_A 72_W 1.187 0.82
187_L 198_A 1.181 0.82
47_L 124_A 1.177 0.82
179_S 182_T 1.173 0.81
240_L 246_I 1.167 0.81
281_V 300_V 1.16 0.80
185_V 201_L 1.152 0.80
352_A 389_I 1.145 0.79
125_F 139_E 1.145 0.79
125_F 129_Q 1.142 0.79
178_E 182_T 1.131 0.78
363_A 381_A 1.13 0.78
257_I 276_L 1.128 0.78
15_V 281_V 1.124 0.77
70_I 88_L 1.116 0.77
237_D 261_M 1.114 0.76
111_V 305_S 1.114 0.76
43_G 58_S 1.113 0.76
105_S 324_I 1.112 0.76
348_Y 415_L 1.111 0.76
260_G 288_E 1.106 0.76
103_V 108_D 1.096 0.75
152_V 324_I 1.093 0.74
21_V 84_L 1.092 0.74
271_N 294_N 1.087 0.74
359_L 379_D 1.086 0.74
9_N 112_L 1.082 0.73
344_T 417_L 1.079 0.73
143_Q 146_F 1.079 0.73
217_E 220_Q 1.077 0.73
247_I 277_V 1.069 0.72
140_R 335_W 1.067 0.72
398_Q 402_D 1.066 0.72
388_L 393_T 1.063 0.72
288_E 325_V 1.063 0.72
200_H 321_A 1.06 0.71
219_A 223_A 1.057 0.71
95_D 98_S 1.057 0.71
183_V 245_I 1.056 0.71
195_E 225_E 1.055 0.71
354_Q 358_E 1.054 0.71
212_A 236_I 1.048 0.70
269_R 273_P 1.045 0.70
143_Q 147_S 1.044 0.70
302_D 306_I 1.04 0.69
144_K 148_V 1.04 0.69
357_D 360_T 1.034 0.69
188_V 236_I 1.031 0.68
7_G 150_K 1.029 0.68
187_L 201_L 1.029 0.68
276_L 297_L 1.028 0.68
116_Q 283_R 1.026 0.68
148_V 324_I 1.02 0.67
184_T 243_A 1.02 0.67
319_V 322_E 1.02 0.67
411_L 414_S 1.018 0.67
344_T 412_R 1.008 0.66
250_T 280_A 1.007 0.66
247_I 279_I 1.004 0.65
409_N 413_D 1.002 0.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1gpjA10.95451000.403Contact Map0.646
1edzA10.70199.90.805Contact Map0.364
1p77A10.617299.90.819Contact Map0.388
3u62A10.557499.90.823Contact Map0.427
1nytA40.617299.80.832Contact Map0.412
3oj0A10.344599.80.842Contact Map0.605
3fbtA40.641199.80.843Contact Map0.495
2eggA20.672299.80.844Contact Map0.547
3o8qA10.638899.70.854Contact Map0.48
2hk9A40.617299.70.856Contact Map0.571
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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