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OPENSEQ.org
MNMG - tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
UniProt: P0A6U3 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10375
Length: 629 (620)
Sequences: 1521
Seq/Len: 2.45
MNMG
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
253_D 257_S 4.935 1.00
577_R 591_N 4.79 1.00
217_Q 241_P 4.75 1.00
453_R 460_D 4.568 1.00
386_L 414_V 4.177 1.00
246_H 292_Q 3.495 1.00
7_F 119_M 3.477 1.00
460_D 464_A 3.222 1.00
407_Q 451_I 3.166 1.00
403_P 408_A 3.147 1.00
210_I 307_I 3.122 1.00
250_K 253_D 3.104 1.00
249_E 291_N 3.085 1.00
66_L 452_G 3.065 1.00
192_L 359_S 3.003 1.00
112_L 120_I 2.818 1.00
259_L 285_M 2.77 1.00
251_T 327_S 2.769 1.00
212_F 307_I 2.707 1.00
393_R 401_W 2.695 1.00
261_R 319_D 2.687 1.00
297_L 331_M 2.686 1.00
453_R 463_W 2.666 1.00
356_T 366_F 2.591 1.00
393_R 398_K 2.587 1.00
476_E 479_R 2.585 1.00
356_T 400_G 2.564 1.00
325_V 334_A 2.537 1.00
74_I 99_A 2.461 1.00
575_D 578_Q 2.436 1.00
211_D 214_V 2.397 1.00
193_R 349_D 2.394 1.00
450_E 454_E 2.376 1.00
471_E 475_R 2.33 1.00
19_T 43_G 2.321 1.00
363_Q 394_L 2.307 1.00
230_M 462_R 2.282 1.00
59_L 382_A 2.252 1.00
210_I 215_L 2.213 1.00
76_Q 107_A 2.213 1.00
248_N 329_Q 2.186 1.00
326_R 334_A 2.163 1.00
215_L 297_L 2.156 1.00
12_I 125_V 2.153 1.00
401_W 451_I 2.122 1.00
73_A 108_V 2.09 1.00
208_R 305_N 2.089 1.00
32_T 118_L 2.087 1.00
450_E 470_L 2.082 1.00
446_L 470_L 2.062 1.00
452_G 458_V 2.057 1.00
127_D 186_R 2.049 0.99
23_M 69_L 2.041 0.99
31_Q 117_N 2.029 0.99
42_L 120_I 2.018 0.99
325_V 336_I 2.012 0.99
192_L 361_F 2.003 0.99
556_Q 560_E 1.993 0.99
195_G 347_F 1.993 0.99
355_P 596_A 1.97 0.99
265_Y 285_M 1.97 0.99
352_D 361_F 1.967 0.99
184_S 194_V 1.965 0.99
207_A 212_F 1.94 0.99
386_L 410_L 1.934 0.99
148_A 151_V 1.92 0.99
206_D 209_T 1.909 0.99
109_R 113_E 1.905 0.99
107_A 110_T 1.897 0.99
10_I 148_A 1.885 0.99
30_Q 396_A 1.871 0.99
214_V 329_Q 1.862 0.99
366_F 394_L 1.86 0.99
193_R 352_D 1.85 0.99
139_V 145_K 1.835 0.99
392_A 396_A 1.831 0.99
386_L 408_A 1.824 0.99
449_T 462_R 1.821 0.99
205_I 210_I 1.817 0.99
127_D 189_E 1.812 0.98
451_I 454_E 1.806 0.98
30_Q 392_A 1.784 0.98
152_V 395_S 1.78 0.98
205_I 334_A 1.773 0.98
63_V 381_A 1.769 0.98
449_T 458_V 1.756 0.98
463_W 467_N 1.739 0.98
167_L 261_R 1.727 0.98
257_S 323_Q 1.725 0.98
74_I 97_A 1.723 0.98
134_R 363_Q 1.706 0.98
519_T 522_K 1.705 0.98
386_L 403_P 1.704 0.97
59_L 428_M 1.702 0.97
76_Q 111_A 1.701 0.97
476_E 520_Y 1.697 0.97
540_E 544_I 1.664 0.97
481_K 510_E 1.661 0.97
259_L 265_Y 1.647 0.97
589_K 604_I 1.64 0.97
160_D 188_R 1.637 0.97
570_L 591_N 1.629 0.96
510_E 546_V 1.629 0.96
150_A 395_S 1.624 0.96
354_K 358_E 1.624 0.96
150_A 394_L 1.613 0.96
219_H 241_P 1.612 0.96
549_E 553_A 1.612 0.96
570_L 574_L 1.608 0.96
479_R 520_Y 1.604 0.96
28_M 393_R 1.602 0.96
586_V 607_V 1.579 0.96
250_K 323_Q 1.566 0.95
60_V 70_M 1.564 0.95
150_A 366_F 1.561 0.95
445_D 469_K 1.547 0.95
586_V 611_A 1.546 0.95
591_N 594_K 1.546 0.95
574_L 579_V 1.539 0.95
171_S 181_I 1.532 0.95
127_D 139_V 1.53 0.94
203_P 324_I 1.52 0.94
207_A 307_I 1.508 0.94
393_R 454_E 1.502 0.94
393_R 396_A 1.499 0.94
498_H 522_K 1.497 0.94
73_A 111_A 1.493 0.93
297_L 307_I 1.491 0.93
180_S 184_S 1.491 0.93
211_D 333_N 1.488 0.93
188_R 194_V 1.485 0.93
473_I 542_V 1.482 0.93
163_I 172_G 1.474 0.93
39_I 120_I 1.469 0.93
63_V 385_L 1.457 0.92
131_E 136_V 1.449 0.92
6_P 147_R 1.448 0.92
51_I 70_M 1.444 0.92
467_N 470_L 1.443 0.92
206_D 305_N 1.432 0.92
168_D 318_F 1.425 0.91
254_V 327_S 1.42 0.91
253_D 256_R 1.419 0.91
203_P 325_V 1.414 0.91
33_L 121_F 1.41 0.91
249_E 253_D 1.41 0.91
152_V 366_F 1.407 0.91
401_W 455_L 1.406 0.91
584_N 588_A 1.404 0.90
599_G 603_R 1.401 0.90
12_I 153_L 1.395 0.90
385_L 389_L 1.382 0.89
78_G 81_F 1.379 0.89
69_L 73_A 1.37 0.89
35_L 146_F 1.362 0.89
314_T 340_G 1.361 0.89
464_A 468_E 1.355 0.88
472_N 538_A 1.351 0.88
214_V 332_E 1.346 0.88
210_I 297_L 1.346 0.88
73_A 107_A 1.323 0.87
460_D 463_W 1.312 0.86
130_V 190_L 1.309 0.86
449_T 466_F 1.307 0.86
446_L 473_I 1.307 0.86
350_P 421_G 1.305 0.86
562_Q 599_G 1.3 0.85
348_F 367_F 1.297 0.85
129_I 145_K 1.295 0.85
459_D 462_R 1.294 0.85
35_L 125_V 1.291 0.85
569_L 596_A 1.275 0.84
83_I 441_E 1.27 0.83
230_M 458_V 1.269 0.83
484_W 508_S 1.26 0.83
230_M 449_T 1.259 0.83
129_I 136_V 1.258 0.83
158_F 174_R 1.257 0.83
186_R 189_E 1.257 0.83
45_M 99_A 1.252 0.82
128_L 137_G 1.252 0.82
79_I 302_L 1.251 0.82
77_A 107_A 1.249 0.82
215_L 245_T 1.242 0.81
9_V 25_A 1.241 0.81
500_T 517_E 1.24 0.81
261_R 320_V 1.239 0.81
258_N 320_V 1.238 0.81
468_E 472_N 1.237 0.81
126_E 182_P 1.236 0.81
478_Q 481_K 1.235 0.81
185_R 189_E 1.233 0.81
312_I 325_V 1.227 0.80
80_Q 220_G 1.226 0.80
210_I 331_M 1.223 0.80
25_A 30_Q 1.221 0.80
69_L 108_V 1.22 0.80
35_L 121_F 1.219 0.80
322_M 336_I 1.218 0.80
587_I 591_N 1.212 0.79
207_A 304_S 1.21 0.79
519_T 543_E 1.203 0.79
23_M 115_Q 1.202 0.78
398_K 454_E 1.191 0.78
23_M 27_R 1.182 0.77
240_V 302_L 1.182 0.77
358_E 394_L 1.177 0.76
354_K 596_A 1.169 0.76
30_Q 395_S 1.169 0.76
38_N 41_T 1.165 0.75
608_T 611_A 1.165 0.75
478_Q 482_S 1.164 0.75
69_L 111_A 1.161 0.75
153_L 187_L 1.157 0.75
27_R 69_L 1.156 0.75
77_A 103_L 1.152 0.74
174_R 177_D 1.148 0.74
422_T 605_S 1.148 0.74
474_E 478_Q 1.147 0.74
326_R 335_K 1.146 0.74
165_I 339_P 1.146 0.74
206_D 335_K 1.145 0.74
115_Q 118_L 1.142 0.73
28_M 455_L 1.142 0.73
446_L 545_Q 1.141 0.73
566_E 603_R 1.14 0.73
54_I 429_F 1.138 0.73
37_H 124_A 1.138 0.73
258_N 324_I 1.122 0.71
103_L 106_Q 1.118 0.71
581_G 587_I 1.111 0.70
463_W 466_F 1.111 0.70
470_L 474_E 1.108 0.70
162_K 169_N 1.108 0.70
553_A 556_Q 1.106 0.70
97_A 220_G 1.101 0.69
613_S 616_L 1.098 0.69
541_Q 544_I 1.096 0.69
476_E 542_V 1.095 0.69
412_V 433_A 1.094 0.69
315_S 342_A 1.093 0.69
215_L 328_M 1.092 0.68
577_R 587_I 1.09 0.68
95_T 310_N 1.089 0.68
10_I 33_L 1.088 0.68
250_K 257_S 1.088 0.68
162_K 180_S 1.085 0.68
39_I 109_R 1.083 0.68
102_V 303_T 1.082 0.67
128_L 148_A 1.08 0.67
128_L 151_V 1.078 0.67
39_I 122_Q 1.076 0.67
171_S 179_P 1.075 0.67
95_T 441_E 1.073 0.66
101_R 300_E 1.07 0.66
364_G 394_L 1.069 0.66
160_D 194_V 1.069 0.66
535_D 538_A 1.068 0.66
574_L 618_W 1.068 0.66
63_V 579_V 1.068 0.66
472_N 535_D 1.064 0.66
446_L 541_Q 1.063 0.65
126_E 141_Q 1.06 0.65
220_G 239_Q 1.059 0.65
130_V 362_I 1.059 0.65
186_R 190_L 1.056 0.65
203_P 340_G 1.052 0.64
9_V 395_S 1.051 0.64
477_R 545_Q 1.05 0.64
263_P 266_A 1.045 0.63
72_K 230_M 1.041 0.63
19_T 108_V 1.041 0.63
573_T 594_K 1.04 0.63
39_I 337_V 1.039 0.63
524_T 539_A 1.039 0.63
130_V 133_D 1.039 0.63
25_A 32_T 1.038 0.63
265_Y 282_D 1.038 0.63
598_I 616_L 1.036 0.62
131_E 147_R 1.035 0.62
181_I 184_S 1.034 0.62
598_I 612_I 1.033 0.62
464_A 467_N 1.033 0.62
122_Q 306_E 1.032 0.62
203_P 334_A 1.03 0.62
136_V 147_R 1.029 0.62
572_A 596_A 1.023 0.61
248_N 252_H 1.023 0.61
51_I 60_V 1.018 0.60
469_K 537_Q 1.016 0.60
79_I 240_V 1.013 0.60
450_E 453_R 1.01 0.60
306_E 338_R 1.007 0.59
306_E 337_V 1.007 0.59
123_Q 140_T 1.005 0.59
410_L 414_V 1.004 0.59
560_E 563_L 1.003 0.59
468_E 471_E 1.003 0.59
6_P 149_K 1.001 0.59
312_I 328_M 1 0.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3cesA40.99681000.323Contact Map0.793
3cp8A40.98251000.353Contact Map0.749
2zxiA40.9651000.393Contact Map0.804
3g5sA10.64551000.68Contact Map0.533
3nlcA10.60571000.889Contact Map0.474
3v76A10.55331000.895Contact Map0.432
2culA10.35451000.905Contact Map0.772
2i0zA10.569299.80.934Contact Map0.63
2gqfA10.559699.70.94Contact Map0.579
1qo8A20.564499.70.942Contact Map0.614
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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